<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31376

Description Mediator of RNA polymerase II transcription subunit 12
SequenceMLSMLQEKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLSDEFGTAHNSNVNASKVSAFFSGVLAKKEELMTLPDTGRKKQQINCKDNFWPVSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQRATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQEYYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVIPVPSMASPLPPIHSPANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSVVGGAAGAGAAVPPGSTISGIGSQFEDSRNALKYWKYCHQLSKYMYEESLLDRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSERLCRKMAYIVSKKLAQLLNTVVEQQTIKELDEPKLQQDPYELALQEQMSCPHHRDIVLYLSTILQIITIECPTALVWSGIAAHRAPSSLLGSPLDHLPLAPSVLPMPTRCPRTNHEIRRQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYTSVLATLDHLDTHCFDRMEHNNSIDTLYAQIFPSPTVSRRREEDQVEPRPPYEPKQDKDTVRILCEWAVSGQRWGEHRAMVVAILLDKRQIDVTSTPADQQSSDKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFTNLVQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPPPPAPPPTTTHGFDDDGFGGGLDFKHNEFDDSNVDDDLDKLVQNIKEKGQQHEAPDSPKIGPPGDGETNPGGSISRHYVYTKHFPIPQDDPSMSSYSSESNQRYILLFGVGKERDEKKHAVKKMSKEIGKLFTKKFSIDVAAAGHVKKHSRNEFNFEATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQEHVAFLFDLMELALNIYSLLELCDSLLKELPEVEHQLQLKKSNLVRSYTTSLALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRHVSNPSECTSAERCIIAYLSDLHESCVLLQGKEQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQGYNPLLLQELFMAPRRGGKLDPHWLGTLHESPANVYSFVSNALIAVCRETDNERLNDVALACAELTASCNVLSEEWIYALQSLCSGSKSPRYPHLGGQVDIGQLKTHNALAVFVCILVARHCFSLADFVSKFALPTLARSVSAGGAELSVDAEAGARLTCHLVLKLFKTLEIPQPGMYSVSTSPNPLHAVGNDFSIRLSCDRHLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSGTSSGGLGGAFGSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEALQQPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEMLTAKQAQRVLHMICYPEPEFNIISELDQRSMIVRILENLGQWTLRISWLDLQLMYRQSLSNNAELNVWLDTVARAAIDVFHMEEVVLPGAVKATHKPKPSTWLVAPLIAKLTPAVQGRILRVAGQVLESMNYFSKVSKSDCNSSGSGDEREKSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEYKENLLVSLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQKNSTPTTDWAILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSDDERHYMNLMKKLKKEIGEKNNASIRVIRQLLPLYKQPTEVIACEHSGMDTKGNKICDIDKKQLRISDKQRISVWDILEGHKNPAPLSWVWFGAVKLERKPLTYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPVPEKICIKDEMKADTPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLAQQPQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNMQQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVNPNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQQSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY
Length2531
PositionKinase
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.06
Grand average of hydropathy-0.538
Instability index55.38
Isoelectric point7.44
Molecular weight279495.85
Publications
PubMed=11171343
PubMed=10731132
PubMed=12537572
PubMed=12537569
PubMed=12835386
PubMed=17290221
PubMed=17372656
PubMed=18327897

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for leg and eye development and macrochaete specification or differentiation. 
GO - Cellular Component
core mediator complex	GO:0070847	IPI:FlyBase
mediator complex	GO:0016592	IDA:UniProtKB
polytene chromosome	GO:0005700	IDA:FlyBase

GO - Biological Function
transcription coactivator activity	GO:0003713	IBA:GO_Central
transcription coregulator activity	GO:0003712	IDA:UniProtKB

GO - Biological Process
chaeta development	GO:0022416	IMFlyBase
compound eye development	GO:0048749	IMFlyBase
ecdysone-mediated induction of salivary gland cell autophagic cell death	GO:0035072	IMFlyBase
imaginal disc-derived leg segmentation	GO:0036011	IMFlyBase
positive regulation of canonical Wnt signaling pathway	GO:0090263	IMFlyBase
regulation of transcription by RNA polymerase II	GO:0006357	IDA:UniProtKB
sex comb development	GO:0045498	IMFlyBase









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31376
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
            13|     781.63|      59|      59|    2224|    2282|       1
---------------------------------------------------------------------------
 1907- 1971 (58.66/16.56)	.QSPS.AVVGGTGRGRGKGtttrkrkP.K.N..PKtppvV.....NT.....Q....Q......QQPQ..............LAQQP...QQpQNV..QQQQ.LQQQQQ......QQ...
 1972- 2004 (47.12/11.85)	....................................................QHM..Q......QQHM..............QQQQM...QP.NQM..GQMP.MNMPMN......MQQFA
 2005- 2028 (37.49/ 7.92)	PNP...........................N.nM....................M..Q......QNAM..............LQQQQ...QQ...............QM......QQM..
 2029- 2100 (44.02/10.58)	..GNN.PMQQQLNVG.GGN.......G.QpNpqMN....FmqqgpGGggagpQGM..PgqqqqwHNAP..............QQQQP...PQ...........PYHNQYaphqqnMQS..
 2101- 2149 (57.05/15.90)	....N.R....IERPPLNA...........N.sKQ....A.....LS.....QML..R......QRQP..............FQQQA...QQ.GPG..GGFN.PMQQQ.......PQASQ
 2150- 2206 (69.99/21.19)	QQP.......................G.P.Q..QQ....M.....NP.....NQM..R......QQQMnpqqnpqsvaafnaMQQQ....PQ.QNA..QQQQ.MNPNQQ......QQQQF
 2207- 2261 (98.63/32.89)	MRGGN......MRPGMAPN.......QmN.Q..MN....M.....GG.....QGM..S......QNPM..............MQQQI...PQ.NMV..GMVN.PNANQM......MQSGG
 2262- 2324 (102.73/34.56)	AQGGN.GVGVGVGVGVGGA.......G.N.N..PN....M.....GM.....GGM..P......QQGM..............IQQQPqqqPQ.QQV..QFQNfQNQYQQ......QQQQG
 2325- 2362 (64.85/19.09)	MQQQG.G.GAGVGVGVGMA.......P.N.Q....................................................QQQQ...QA.NMM..GNFN.P...QM.......QQ..
 2363- 2404 (40.28/ 9.06)	..............G..NR.......N.N.P..DF....M.....AA.....AAV..A......QQQQ..............QQQQ....QQ.RVVpgGMMA.GNRNQY......MN...
 2405- 2436 (48.24/12.31)	.QAPN..VTMSTMMGPG.........................................................................PG.GVV..GQVP.PYARQ........QSAG
 2437- 2468 (44.61/10.83)	..GGKpGV.........................LN..........TQ.....QQF..Q......QQQQ..............QQQQL...R............H...QM......MQLQG
 2469- 2523 (67.96/20.36)	MGGGA.GGGMGAGPQQGGG.......A............V.....GG..gagGGMvpQ......QQSM..............NQQQT...P..NLV..AQLQ.RQ.NMM......GQ...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     263.40|      87|     154|    1475|    1566|       2
---------------------------------------------------------------------------
 1475- 1565 (141.86/92.67)	LDQRSMIVRILENL.GQWTLRISWL.DLQLMYRQSLSNNAELNV..WLD.TVARAAI.DVFHMEEVVLPGAVKATHK.PKPST.WlvAPLIAKLtpAVQ
 1625- 1719 (121.54/68.47)	LDYQPFLGLILTCLkGQDEYKENLLvSLYAQLSQCLQSFAELDTigGIDePQAREEIlDALQLRFSLVGGMFEAIQKnSTPTTdW..AILLAQL..VCQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.64|      26|     443|     967|     992|       3
---------------------------------------------------------------------------
  967-  992 (44.05/31.84)	SLLELCDSLLKELPEVEHQLQ..LKKSN
 1412- 1439 (38.59/26.81)	SLLEFAQAVLKQICAQEHVLErcLKNAE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.46|      12|      15|    1101|    1112|       8
---------------------------------------------------------------------------
 1101- 1112 (21.64/12.03)	NPLLLQELFMAP
 1119- 1130 (24.82/15.03)	DPHWLGTLHESP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31376 with Med1 domain of Kingdom Metazoa
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