<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31375

Description Mediator of RNA polymerase II transcription subunit 12
SequenceMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTPIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKMEDTGMSESMEIDHNSSANFDEMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY
Length2173
PositionKinase
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Actinopterygii> Neopterygii> Teleostei> Ostariophysi> Cypriniformes> Danionidae> Danioninae> Danio.
Aromaticity0.07
Grand average of hydropathy-0.535
Instability index56.01
Isoelectric point6.90
Molecular weight243989.28
Publications
PubMed=16344459
PubMed=16712834
PubMed=17088561

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for development of the body axis, brain, ear, kidney, forelimb and neural crest and for pigmentation. Acts as a coactivator for sox9a and/or sox9b promoting the expression of several neuronal determination genes. 
ECO:0000250	
ECO:0000269	PubMed:16344459
ECO:0000269	PubMed:16712834
ECO:0000269	PubMed:17088561
GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central
GO - Biological Function
beta-catenin binding	GO:0008013	IEA:InterPro
DNA-binding transcription factor activity	GO:0003700	IGI:ZFIN
transcription coactivator activity	GO:0003713	IBA:GO_Central
GO - Biological Process
central nervous system neuron development	GO:0021954	IMZFIN
ear development	GO:0043583	IMZFIN
embryonic neurocranium morphogenesis	GO:0048702	IMZFIN
embryonic pectoral fin morphogenesis	GO:0035118	IMZFIN
embryonic viscerocranium morphogenesis	GO:0048703	IMZFIN
endoderm development	GO:0007492	IMZFIN
forebrain development	GO:0030900	IMZFIN
habenula development	GO:0021986	IMZFIN
heart development	GO:0007507	IMZFIN
iridophore differentiation	GO:0050935	IMZFIN
kidney development	GO:0001822	IMZFIN
midbrain development	GO:0030901	IMZFIN
neural crest cell development	GO:0014032	IMZFIN
neuron differentiation	GO:0030182	IMZFIN
pineal gland development	GO:0021982	IMZFIN
positive regulation of transcription, DNA-templated	GO:0045893	IGI:ZFIN
regulation of myeloid leukocyte differentiation	GO:0002761	IMZFIN
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
rhombomere boundary formation	GO:0021654	IMZFIN
transcriptional activation by promoter-enhancer looping	GO:0071733	IMZFIN
ventricular system development	GO:0021591	IMZFIN

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31375
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     252.95|      31|      31|    2045|    2075|       1
---------------------------------------------------------------------------
 1909- 1942 (36.21/12.93)	PN..P......QysSMQ............PAQN..IPQgyTSYGSHmG...M....QPHPSQT
 1943- 1985 (26.97/ 7.55)	PGMVP.nsygnQ..GFQtghpatnptmvdSIRQ..MQQ..RPGYVH.....Q....QAP....
 2024- 2062 (56.35/24.64)	PNMRPnqmmeqQ..QQQ............QQQQqqQQQ..QQQQVH.Q...Q....QQHIRQG
 2063- 2094 (50.18/21.05)	PVFRP......Q..QQQ............QVQQqvQQQ.vQQQQV..Q...Q....QQ.VQQQ
 2095- 2132 (33.75/11.49)	..VQP......QqvQQQ............QVQQ..QQQ..QQVSAA.QppaQalgmQALPPQQ
 2133- 2164 (49.50/20.66)	PMFQR......Q..GMQ............QTQQ..QQQ..TAALVR.Q..lQ....QQLSSTQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     235.03|      69|      94|     304|     376|       2
---------------------------------------------------------------------------
  304-  376 (118.89/87.69)	LNLLLSDGSIGPGPGGHQAHGisaQQGNALPPTPTSQPAGGNQPQTPFTDFYICpQHRPVVFGLSCMLQSI.VL
  399-  468 (116.15/73.66)	LDLLPIAPSNLPMPGGNSTFN...QQVRAKVREIEEQIKERGQAVEFRWSFDKC.QETTAGFTISRVLHTLeVL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     171.73|      44|      95|    1632|    1676|       3
---------------------------------------------------------------------------
 1632- 1676 (70.77/42.81)	LPLPKQTRDVIT...cEPQGSLIDTKGNKIAGFEKEGL.....QVST.KQK...ISP
 1687- 1730 (49.39/24.40)	APLSWGWFGTIR..........VDRKVTKFE..EQQRLllyhtHIKP.KQRsyyLAP
 1731- 1773 (51.57/25.78)	LNLPQEEEEPLTpvppEPDKKL.DT.....AKVEKSVS.....NVPTkKKK...KKP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     132.12|      40|      52|     645|     696|       4
---------------------------------------------------------------------------
  639-  690 (59.24/63.99)	RSPSDEPSDESERKDQDAGSSVKmeDTGMSESmeidhnssanFDEMFSPPMH
  691-  730 (72.88/47.25)	CESKGSPSPEKQAQEQESKSTAK..DKGMDPA..........FPLVYEQPRH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     126.58|      30|      52|    1803|    1833|       5
---------------------------------------------------------------------------
 1806- 1844 (49.02/25.94)	TNMSYRMGYQAPM.NMyAQNQPLPPGG.PGleppfrprV.PM
 1845- 1872 (43.09/18.56)	NKMPPRPNYTMP..NM....QTAGMGNlMG........MeKQ
 1873- 1902 (34.47/13.43)	....YQMNYK.PMpNM.AQGQMLRHQL.QG....hnhlI.GQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     150.04|      41|      56|    1172|    1224|       7
---------------------------------------------------------------------------
 1172- 1214 (63.03/42.85)	PCPQD..GTKSDKSIVGIRSSCDRHlLAASQNSIVVGAVFaVLKA
 1231- 1269 (61.88/48.02)	PAGLD..DIGEDD..MGSKKSGGRN.VSIETASLVVYAKY.VLKS
 1271- 1297 (25.13/ 7.41)	.CHQEwvGERCLKSL.....SEDS...SALQDPVLV.........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     181.38|      54|      57|     990|    1046|       8
---------------------------------------------------------------------------
  990- 1046 (88.49/53.03)	IDPSDSNMLWDQMFMIDAITnPTAHNLNHSMLGKITNELNDSPANRYSF...VCNVlmDV
 1048- 1104 (92.89/47.00)	VDHRDPERVNDIGILCAELT.AYCRSLSAEWLGVLKALCCSSNNGNCGFndlLCNV..DV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.03|      16|      30|    1456|    1471|       9
---------------------------------------------------------------------------
 1456- 1471 (27.47/17.81)	KERDRQKQKSM.SLLSQ
 1483- 1499 (23.56/14.01)	KGQDEQREGLLtSLYSQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.43|      13|      30|     841|     853|      10
---------------------------------------------------------------------------
  841-  853 (23.88/12.73)	FALGMSYHLPLVQ
  874-  886 (18.55/ 8.29)	FAIQLLNELSLVE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.46|      12|      30|    1122|    1133|      11
---------------------------------------------------------------------------
 1122- 1133 (21.26/12.88)	AR.QCLLLEDLVR
 1154- 1166 (18.20/ 9.98)	ARlTCRILLHLFR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     238.94|      82|    1315|      30|     122|      13
---------------------------------------------------------------------------
   30-  122 (125.48/128.54)	KQ.KEDELTAL..NVKQGFNNQPAVSGDEHGSakNVNFNSSKIS.SNFS.SIIAEKLRcntfPDTGKrkpqvNQKDNFWLVT...ARSQSSINNWFTDLAG
 1352- 1441 (113.46/86.11)	KQsSNNELYSLleNIAKATIEVFQKSAEMNSS..NPSWNGSAVSgSSVSnSNSASKLK....PVLSS.....SERSGVWLVApliGKLPTSVQGHVLKAAG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      35.30|      11|      31|     946|     959|      14
---------------------------------------------------------------------------
  946-  959 (13.73/19.94)	CSSAERCIlaYlYD
  979-  989 (21.58/13.89)	CSKVKNSI..Y.YN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.81|      19|      31|     760|     778|      16
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  760-  778 (30.22/21.10)	GKQRDEARHAIKKITKDIL
  794-  812 (31.59/22.44)	GQKRKRSKPEAFPTAEDIF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.37|      21|      23|    1545|    1567|      17
---------------------------------------------------------------------------
 1545- 1567 (30.56/27.03)	QQTTewAVL.LLDIISSGTV..DMQS
 1570- 1593 (25.81/14.37)	ELFT..TVLdMLSVLINGTLaaDMSS
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31375 with Med12 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ASNSHLPRPRSPSDEPSDESERKDQDAGSSVKMEDTGMSESMEIDHNSSANFDEMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESA
2) KRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKN
3) MGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQ
4) QMNYKPMPNMAQGQMLRHQLQGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQ
5) QQVSAAQPPAQALGMQALPPQQPMFQRQGMQ
6) YYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMS
630
15
1812
1874
2111
1726
745
61
1872
2068
2141
1809










Molecular Recognition Features






MoRF SequenceStartStop




1) AAFGV
2
6