<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31318

Description Mediator of RNA polymerase II transcription subunit 37a
SequenceMARSFGANSTVVLAIIFFGCLFALSSAIEEATKLGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFTDSERLIGEAAKNQAAVNPERTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNKDGKPYIQVKIKDGETKVFSPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGLDKKGGEKNILVFDLGGGTFDVSVLTIDNGVFEVLSTNGDTHLGGEDFDHRVMEYFIKLIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEPNKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRLLGKFDLNGIPPAPRGTPQIEVTFEVDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIDRMVKEAEEFAEEDKKVKEKIDARNALETYVYNMKNQVNDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQRSGGAPGGAGGESSTEEEDESHDEL
Length669
PositionUnknown
OrganismArabidopsis thaliana (Mouse-ear cress)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Streptophyta> Embryophyta> Tracheophyta> Spermatophyta> Magnoliopsida> eudicotyledons> Gunneridae> Pentapetalae> rosids> malvids> Brassicales> Brassicaceae> Camelineae> Arabidopsis.
Aromaticity0.06
Grand average of hydropathy-0.463
Instability index31.56
Isoelectric point5.08
Molecular weight73628.39
Publications
PubMed=11130714
PubMed=27862469
PubMed=14593172
PubMed=8281187
PubMed=11599561
PubMed=11402207
PubMed=17560376
PubMed=17272265
PubMed=19763086
PubMed=20080634
PubMed=21909944
PubMed=22021418
PubMed=23624714
PubMed=24486762
PubMed=26186593
PubMed=26251880
PubMed=31410484

Function

Annotated function In cooperation with other chaperones, Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions (Probable). Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation (PubMed:20080634). Involved in sperm nuclear fusion with central cell polar nuclei at fertilization, which is critical for normal endosperm nuclear proliferation (PubMed:31410484). Required for pollen development and pollen tube growth (PubMed:24486762). Possesses ATPase activity in vitro (PubMed:26186593). 
ECO:0000269	PubMed:20080634
ECO:0000269	PubMed:24486762
ECO:0000269	PubMed:26186593
ECO:0000269	PubMed:31410484
ECO:0000305	PubMed:11402207
GO - Cellular Component
cell wall	GO:0005618	IDA:TAIR
chloroplast	GO:0009507	IDA:TAIR
cytoplasm	GO:0005737	IBA:GO_Central
cytosol	GO:0005829	IDA:TAIR
endoplasmic reticulum	GO:0005783	IDA:TAIR
endoplasmic reticulum chaperone complex	GO:0034663	IBA:GO_Central
endoplasmic reticulum lumen	GO:0005788	IBA:GO_Central
Golgi apparatus	GO:0005794	IDA:TAIR
mediator complex	GO:0016592	IDA:UniProtKB
membrane	GO:0016020	IBA:GO_Central
nucleus	GO:0005634	IBA:GO_Central
plasma membrane	GO:0005886	IDA:TAIR
plasmodesma	GO:0009506	IDA:TAIR
secretory vesicle	GO:0099503	HDA:TAIR
vacuolar membrane	GO:0005774	IDA:TAIR
vacuole	GO:0005773	IDA:TAIR
GO - Biological Function
ATP binding	GO:0005524	IBA:GO_Central
ATPase activity	GO:0016887	IBA:GO_Central
heat shock protein binding	GO:0031072	IBA:GO_Central
misfolded protein binding	GO:0051787	IBA:GO_Central
protein folding chaperone	GO:0044183	IBA:GO_Central
unfolded protein binding	GO:0051082	IBA:GO_Central
GO - Biological Process
cellular response to unfolded protein	GO:0034620	IBA:GO_Central
chaperone cofactor-dependent protein refolding	GO:0051085	IBA:GO_Central
endoplasmic reticulum unfolded protein response	GO:0030968	IBA:GO_Central
protein refolding	GO:0042026	IBA:GO_Central
ubiquitin-dependent ERAD pathway	GO:0030433	IMTAIR

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31318
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      99.94|      26|      27|     579|     604|       1
---------------------------------------------------------------------------
  550-  569 (22.93/ 9.69)	.....VKEAEEFAEE.DKKVKEK.IDA
  579-  604 (41.89/23.61)	NMKNQVNDKDKLADKLEGDEKEK.IEA
  608-  633 (35.12/18.64)	EALEWL.DENQNSEKEEYDEKLKeVEA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.73|      11|      27|     231|     241|       2
---------------------------------------------------------------------------
  231-  241 (20.38/13.74)	LGGGTFDVSVL
  259-  269 (22.35/15.86)	LGGEDFDHRVM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     105.01|      27|      27|      87|     113|       3
---------------------------------------------------------------------------
   68-   84 (18.85/ 7.43)	.....ITPSWVG.FtDSERLIG......E
   87-  113 (46.50/28.06)	KNQAAVNPERTV.F.DVKRLIGRKFEDKE
  116-  142 (39.66/22.96)	KDRKLV.PYQIVnK.DGKPYIQVKIKDGE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      90.99|      26|      27|     386|     411|       4
---------------------------------------------------------------------------
  196-  222 (26.61/14.66)	I..AGLNVARII.NePTaAA.IAYGLDKKGG.
  386-  410 (38.60/24.76)	...EGKEPNKGV.N.PD.EA.VAYGAAVQGGI
  411-  440 (25.78/13.95)	LsgEGGDETKDIlL.LD.VApLTLGIETVGGV
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31318 with Med37 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKE
2) ITAVYQRSGGAPGGAGGESSTEEEDESHDEL
586
639
630
669










Molecular Recognition Features






MoRF SequenceStartStop




1) EVEAVCN
630
636