<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31316

Description Mediator of RNA polymerase II transcription subunit 36a
SequenceMRPPLTGSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRGGARGGRGPAGRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKTEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKSGGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKPKAATAA
Length320
PositionUnknown
OrganismArabidopsis thaliana (Mouse-ear cress)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Streptophyta> Embryophyta> Tracheophyta> Spermatophyta> Magnoliopsida> eudicotyledons> Gunneridae> Pentapetalae> rosids> malvids> Brassicales> Brassicaceae> Camelineae> Arabidopsis.
Aromaticity0.07
Grand average of hydropathy-0.395
Instability index30.26
Isoelectric point10.08
Molecular weight33652.90
Publications
PubMed=10829025
PubMed=10617198
PubMed=27862469
PubMed=14593172
PubMed=10806224
PubMed=17666011
PubMed=17560376
PubMed=17576925
PubMed=18322146
PubMed=21785141
PubMed=22021418
PubMed=22349738
PubMed=29163603
PubMed=30307032

Function

Annotated function S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (Probable). Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (Probable). Site specificity is provided by a guide RNA that base pairs with the substrate (Probable). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (Probable). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity). Acts as negative regulator of expression of immune responsive genes, including pathogenesis-related gene 1 (PR1), and of resistance against bacterial pathogen (PubMed:30307032). Binds to MED19A, a positive regulator of PR1 expression, to repress the activator activity of MED19A (PubMed:30307032). In response to the bacterial pathogen-associated molecular pattern (PAMP) elf18, associates with the long non-coding RNA (lncRNA) ELENA1 (At4g16355), and releases its repression of MED19A (PubMed:30307032). Possesses ribonuclease activity toward rRNA in vitro (PubMed:29163603). Binds phosphoinositides, phospholipids and phosphatidic acid in vitro (PubMed:29163603). 
ECO:0000250	UniProtKB:P15646
ECO:0000269	PubMed:29163603
ECO:0000269	PubMed:30307032
ECO:0000305	PubMed:10829025
GO - Cellular Component
box C/D RNP complex	GO:0031428	IBA:GO_Central
Cajal body	GO:0015030	IBA:GO_Central
cytosol	GO:0005829	HDA:TAIR
mediator complex	GO:0016592	IDA:UniProtKB
nucleolus	GO:0005730	IDA:TAIR
small-subunit processome	GO:0032040	IBA:GO_Central
GO - Biological Function
histone-glutamine methyltransferase activity	GO:1990259	IBA:GO_Central
mRNA binding	GO:0003729	IDA:TAIR
ribonuclease activity	GO:0004540	IDA:TAIR
RNA binding	GO:0003723	IDA:TAIR
rRNA methyltransferase activity	GO:0008649	IBA:GO_Central
snoRNA binding	GO:0030515	ISS:TAIR
GO - Biological Process
box C/D RNA 3'-end processing	GO:0000494	IBA:GO_Central
defense response	GO:0006952	IEA:UniProtKB-KW
histone glutamine methylation	GO:1990258	IBA:GO_Central
rRNA methylation	GO:0031167	IBA:GO_Central

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31316
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      93.44|      15|      15|       9|      23|       1
---------------------------------------------------------------------------
    9-   23 (35.90/11.67)	G.GGFSGGR...GRGGYSG
   26-   41 (30.62/ 8.99)	GdGGFSGGR...GGGGRGG
   59-   76 (26.91/ 7.10)	R.GGARGGRgpaGRGGMKG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     219.66|      67|     159|      86|     156|       2
---------------------------------------------------------------------------
   86-  156 (102.35/86.19)	RHAGVFIAKGKEDALvtKNLVPGEAVYNEKRISVQNEDGTKTEYRVWNPFRSKlAAAILGGVdNIWIKPGA
  250-  316 (117.30/82.09)	KSGGHFVISIKANCI..DSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERD.HACVVGGY.RMPKKPKA
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31316 with Med36 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MRPPLTGSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRGGARGGRGPAGRGGMKGGSKV
1
80










Molecular Recognition Features






MoRF SequenceStartStop




1) FSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPP
2) MRPPL
12
1
58
5