<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31275

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMASSSGSKEPTTASLLLSTLGAYSDLAKHLFASIENPSQQKTRTITDTAADKSWKLRGVSDVLAALREVDELLAQRIQLAHLHAANQATIEQLQAKARRRDRDTRQAILELSNINAELAEITRLSEDELASIARAAERPVGHATLLTYAQRLAKYTSAPPGYKLPQVASTSTTVKSEPTKAEQAGGDDGDAQADEIALGPDYNQYAKKAAAYYDPAIPSMPQEMPFPSDAMMRQGILNSADILAGAPMAEQPAEDVAMDEQDELPVADFAASYSHLREQQQRQDEDEDAFDLDLN
Length295
PositionMiddle
OrganismPseudozyma antarctica (strain T-34) (Yeast) (Candida antarctica)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Ustilaginomycotina> Ustilaginomycetes> Ustilaginales> Ustilaginaceae> Moesziomyces.
Aromaticity0.05
Grand average of hydropathy-0.528
Instability index51.66
Isoelectric point4.62
Molecular weight32041.15
Publications
PubMed=23558529

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31275
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     106.44|      32|      55|     133|     166|       1
---------------------------------------------------------------------------
  133-  166 (47.91/35.87)	ARAAERPVGhATLLTYAQRLAKYTSaPPGYKLPQ
  191-  222 (58.53/34.36)	AQADEIALG.PDYNQYAKKAAAYYD.PAIPSMPQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     102.64|      37|      54|      32|      72|       2
---------------------------------------------------------------------------
   32-   72 (48.47/39.70)	ASIENpSQQKTRTiTDTaaDKSWKLRGVSDVLAALREV.....DEL
   88-  129 (54.17/29.70)	ATIEQ.LQAKARR.RDR..DTRQAILELSNINAELAEItrlseDEL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      78.52|      22|      24|     223|     244|       3
---------------------------------------------------------------------------
  223-  244 (40.00/24.84)	EMPFPSDAMMRQGILNSADILA
  250-  271 (38.52/23.68)	EQPAEDVAMDEQDELPVADFAA
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31275 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DILAGAPMAEQPAEDVAMDEQDELPVADFAASYSHLREQQQRQDEDEDAFDLDLN
2) PGYKLPQVASTSTTVKSEPTKAEQAGGDDGDAQADEIALGP
241
160
295
200










Molecular Recognition Features






MoRF SequenceStartStop




1) EDEDAFDLDLN
2) ELPVADFAASYSHLREQQ
3) YYDPAIP
285
263
212
295
280
218