<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31270

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMPSTPHGANLPATTHNVNANGVHRPSPISSDTTAIPGPPSKSIMATDTNGHFQDSAQPATDKNKRSASSPHKLMIGVPPAATPNPPRSSTEHTHPHNDAHNPVQNGNVSSELKGKQKQLDPIHTVSLEQLQQELPLEETDLVSLAALVERLANYGYESLQNLAETLPSLPSSSKRAKIFSTALDVRKQFIKLLVLARWSKDVPDLQKARNIIALLSEQQWQHEDVFAGLTDIRKILPNARMRNADLPTAIDVLQTGTYRRLPASIKDMAVAPPPLTDQQALAIVARLQDALRTRMACRELVPAPLSDYSIHDAKVHFHVRGLFRAQLTASSGGRDQDADRPDQHAESGAADAASSAASANDRWWLLDLSFDATPTGSCAESSLKVFPKRPKKAYRERLRVWGDQELAPRSQATDAALDANADAAPTASEPPHVLSTADTQGESKSAMQQSEPQDASPQPTQPDADPEASFKLPKTRDAPLVRLFAFLQERALHYQMDILQHQAYELCRLNWGSNLRIETAERPRRLTVHYWTHAQGAEGTSSAPDSPAAGGSIQISLVDLPDQAVVTKTLAEIFDDDAPSGCDGASPTAVKRRGLRVIWNAHASVLSDSETSDLDIDPHNLDIEATLSLVIKRHTAALLRNLQRRLLVSDHPVSRLLRPEDCTLCVEHARRQGDDATSFNFLRVELHGRNRQRAARTKRAAAVSTMPPLRLSADPVTGRLLLDSEAASDAADAGLHGSTAPASDFTASILTTRPASARLSEASDRINGSIDALFDVLYRLEVFARVQDWERMASYLGLRTVRKLSLRPQDLAKFGSSLSTDAAPQLFIPLRKSFPGYFLALQPSELAGARIALVYVVQMPDPSGAPSLTVQSIEWLDRVKIANASKAGRGFPADAAMPGPGAGKKRKAASAETSETQEADAGIAVRELSMEELADVHSYCIALVSYFRVEQQLRMRGIPYLHVAGSKADSATPPKWRRRVAPATSDAHVDARDNGLFDDDDDDDEGMEAEAAAEPAGVDSQEQAVETGVAALVPTLCLRAADVLGPAKAHLTKPNVSLRVCDWDESDKTCVQVLVKLRMKSRRFRALHEVVAYASSSDQHAQSAATWIDFDDATCVLAISTRDVDNCIPIFYMQWERVMRMVMLTREVLNASRAWQQRALRARTSCKKPAERVELCRFQLDTVVFSYGTLGVEDGARKLLVRVRWQDPKMEMAAYGAMPVAQNGGYVVEFGSAPVSEVEVEAAKSKDEALSACCGERSTWRRARPP
Length1266
PositionTail
OrganismPseudozyma antarctica (strain T-34) (Yeast) (Candida antarctica)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Ustilaginomycotina> Ustilaginomycetes> Ustilaginales> Ustilaginaceae> Moesziomyces.
Aromaticity0.05
Grand average of hydropathy-0.391
Instability index50.15
Isoelectric point6.29
Molecular weight138195.17
Publications
PubMed=23558529

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats
>MDP31270
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     121.66|      30|      46|     404|     433|       1
---------------------------------------------------------------------------
  341-  364 (29.21/ 9.70)	....PDQHAESGAADA.AS.SAASANDRWW.
  404-  433 (50.85/22.07)	QELAPRSQATDAALDANAD.AAPTASEPPHV
  453-  481 (41.60/16.78)	QDASPQPTQPDA..DPEASfKLPKTRDAPLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     588.16|     146|     151|     657|     807|       2
---------------------------------------------------------------------------
  515-  677 (139.91/80.78)	......LRI...ETAERPRRLTvhywTHAQGAEGTSSAP.DSPaaggsiqislvdLPDQAVVTKTL.........AEIFDD.DAPSGCDGASPTAVKRRGlRVIWNAHASVlSDSETSDlDIdphNL.DIEATLSL...............VIK............RHTAAL.LRNLqRRLLVSdhpvsrllRPEDctlCVEHARRQGDDAT
  678-  823 (224.62/126.02)	SF..NFLRV...ELHGRNRQRA....ARTKRAAAVSTMP.PLR............LSADPVTGRLLLD.......SEAASD.AADAGLHGSTAPASDFTA.SILTTRPASA.RLSEASD.RI...NG.SIDALFDV...............LYRLEVFARVQDWE.RMASYLgLRTV.RKLS.........lRPQD...LAKFGSSLSTDAA
  833-  956 (119.10/59.88)	SFpgYFLALqpsELAG..........ARIALVYVVQ.MPdPSG............APSLTVQSIEWLDrvkianaSKAGRGfPADAAMPGPGAGKK.........RKAASA.ETSETQE.AD...AGiAVREL....................SMEELADVHSYCiALVSY..FRVE.QQLRMR............................
  959- 1064 (104.53/51.18)	....PYLHV.................AGSK..ADSATPP.KWR............RRVAPAT.............SDAHVD.ARDNGLFDDDDDDDEGME.AEAAAEPAGV.DSQEQA...V...ET.GVAALVPTlclraadvlgpakahLTKPNVSLRVCDWD...............................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     129.17|      38|      45|      11|      54|       3
---------------------------------------------------------------------------
   11-   51 (60.90/53.22)	PATTHN.VNANGVHRpSPISSDTTAIPGPPSKSimATDTNGH
   58-   96 (68.26/38.45)	PATDKNkRSASSPHK.LMIGVPPAATPNPPRSS..TEHTHPH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     118.04|      31|    1004|     178|     208|       4
---------------------------------------------------------------------------
  116-  148 (32.13/17.57)	QKQLdpIHTVSLEQLQQELP.LEE.T...............dLVS.LAALV
  149-  185 (20.98/ 8.90)	ER.........LANYGYE..SLQNlA..etlpslpssskrakIFS.TALDV
  186-  232 (39.00/22.91)	RKQF..IKLLVLARWSKDVPDLQK.ArniiallseqqwqhedVFA.GLTDI
  233-  252 (25.93/12.74)	R......KILPNARMRN..ADL.......................pTAIDV
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31270 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AHVDARDNGLFDDDDDDDEGMEAEAAAEPAGVDS
2) MPSTPHGANLPATTHNVNANGVHRPSPISSDTTAIPGPPSKSIMATDTNGHFQDSAQPATDKNKRSASSPHKLMIGVPPAATPNPPRSSTEHTHPHNDAHNPVQNGNVSSELKGKQKQLDPIHTVSLEQL
3) PADAAMPGPGAGKKRKAASAETSETQEADAGIA
4) RLRVWGDQELAPRSQATDAALDANADAAPTASEPPHVLSTADTQGESKSAMQQSEPQDASPQPTQPDADPEASFKL
987
1
892
397
1020
130
924
472










Molecular Recognition Features






MoRF SequenceStartStop




1) ERSTWRRAR
2) PKWRRRV
1256
974
1264
980