<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP30814

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDDVLNEQFGRVERALTTLVDSIAAYNPATQAAIDLVAADDQLSDGLDQLAKHQANHGRIQTLRAEADALEAQLRDSVKKLAELRRELFNTPATTFSPESRAVPFDELLQYAANISRYTVPPTYRERVPTTDKDDDDVGSSGALTNGMSTPAIVPDAVDLPNDSSEAPKAGEDADGAVPDITAEEEEWLRKLKASNLAWYPWPSTEKIRNGILYRLSYWREKGNDLDQFDIPAYLEEQRKKALGNEASLEETPAEPTEEQPHREAARPTRPLSAFTGLDDMDEM
Length284
PositionMiddle
OrganismCochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) (Southern corn leaf blight fungus) (Bipolaris maydis)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Dothideomycetes> Pleosporomycetidae> Pleosporales> Pleosporineae> Pleosporaceae> Bipolaris.
Aromaticity0.06
Grand average of hydropathy-0.697
Instability index41.85
Isoelectric point4.43
Molecular weight31514.32
Publications
PubMed=23236275
PubMed=23357949

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP30814
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      69.82|      22|      23|     135|     156|       1
---------------------------------------------------------------------------
  132-  155 (34.92/21.12)	DkdDDDVGSSGALTNGMSTPAIVP
  156-  179 (34.91/21.12)	DavDLPNDSSEAPKAGEDADGAVP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      75.73|      24|      24|      81|     104|       2
---------------------------------------------------------------------------
   80-  103 (38.88/21.57)	KLAELRRELFNTPATTFSPESRA.V
  104-  128 (36.85/20.13)	PFDELLQYAANISRYTVPPTYRErV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.93|      19|      19|     220|     238|       3
---------------------------------------------------------------------------
  193-  221 (23.95/10.32)	KASNLAWYPWPStekirngilYrLSYWRE
  222-  240 (33.98/17.03)	KGNDLDQFDIPA.........Y.LEEQRK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.74|      24|      28|      21|      45|       4
---------------------------------------------------------------------------
   21-   45 (34.72/26.80)	DSIAAYNpATQAAIDLVAAD.D....QLSD
   48-   76 (32.02/19.01)	DQLAKHQ.ANHGRIQTLRAEaDaleaQLRD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP30814 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) KKALGNEASLEETPAEPTEEQPHREAARPTRPLSAFTGLDDM
2) PTYRERVPTTDKDDDDVGSSGALTNGMSTPAIVPDAVDLPNDSSEAPKAGEDADGAVPDITAEE
240
122
281
185










Molecular Recognition Features






MoRF SequenceStartStop




1) EQPHREAARPTRPLSAFTGL
2) FDIPAYLEEQRKKAL
3) IRNGILYRLSYWREK
4) LQYAANI
5) SLEET
259
229
208
109
248
278
243
222
115
252