<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP30143

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTETPLAVTLPHPAHALESSTNPNGFAGTETDDVPLELLERELPVVYDDQIYLKELLQRVVQTIYAELSEMAETLPGMSDVARKRTLADWVVKTKKQVVKLYAVAKWSRDADVVQKCMNITAFLMGQHKQFQDVMEALRIAKDQLDPARQVPYRPWLRNHDLLTSLDVLTTGSYLRLPTCIKKYIIQPTPLIDSEVRKTLSDIEEAIRYRLRMWEVIPVEMAQYRIADGRVYFMVPHLFEMSLCLRGADLDDGWFFVHVEFLIEVGGDVATLQEFPRIPTGLLKRHITDEADARLAYYLRLPELEQVPPGFEPPSRPKLPPGYVDAPLVRVFNFLQMMSLSYQLEILWYQAQRMRALGWADYLTVQMTQNRKSMTVSYWIRPTAPSIPGQQHRKFKLPLLGGKVIISIVETRGPPQAGPGPDRSPKMRTLAKLQRKAKLGDATPSDEVEGLHFEMKWEPEKGALGARVPPDQLVLPKEHLQIKPECLDFEAMLKAIIENHTAAILATFQHQLQTSHARAVFGPPGVVTRITELGSPALRVHLCADEVVIVTIDARTGRITLRDSGDLAAAGRVSRFNVISEKINDDPTVLLNALFRLRFMTIIDLAESKARYLGLQCYRMRHFPQQEWAKLGSDARVFLFIQLTNFPNHYLVIVVTDDKYRFALITTSTDREVMFAHMVLQDIAWLDYDRIRRETLAVSKMTENLNSGVKTGRKRMRLAGLTLGQPGFPDFDLETQMLRELYSYCCARVAYMNVERQLKLQGIPFTHVNPTSGMPVTQDQAHAQSSLARSVPGICVQSKDILSGAPAAEAAMPNIRVVPLGWWSEQKAQVITIVKLKYVQQPMGRRIGGTIIRPSKRIIYDTKEAIVSFLSENVDTCVGEFLEEWARVSKMVVIAREVAQMSKAKNWHDIRLLSFDLQTVEFAYAENYTVSITCEDQISGTGGNFVLRFARRKLPPELTSSSGGEHMDVDIAEEDLFNPHDDAEPFFRSILRGRLAPSLNRLVALLRETLPIVTELEAIRREEERANRVVDVYPKAAGWYRLLYGDLRHALDFRLMTGQRVAILDGSCSFYASVNSTKSSLGGSNSDSMAVGLQPLPHFNSIVTAVVKKAMATGEVRAGKIARVDRGVICECADVRKLARAIHSRVADILVAAGGSNSGASIV
Length1163
PositionTail
OrganismAgaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC 10392) (White button mushroom)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Agaricomycotina> Agaricomycetes> Agaricomycetidae> Agaricales> Agaricaceae> Agaricus.
Aromaticity0.08
Grand average of hydropathy-0.141
Instability index41.33
Isoelectric point8.42
Molecular weight130852.79
Publications
PubMed=23045686

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP30143
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     197.07|      68|     115|     960|    1038|       1
---------------------------------------------------------------------------
  960- 1038 (95.98/83.49)	SSSGG...EHMDVDIaeEDLfnPHddaepfFRSI....LRGRLAPSLNRLVALLRETLPIVTELEAIRREeERA..NRVVDVYPKAAG
 1079- 1155 (101.09/59.48)	SSLGGsnsDSMAVGL..QPL..PH......FNSIvtavVKKAMATGEVRAGKIARVDRGVICECADVRKL.ARAihSRVADILVAAGG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     158.40|      43|     115|     739|     801|       2
---------------------------------------------------------------------------
  739-  781 (74.20/42.44)	REL.YSYCCARVAYM..NVERQLKLQGIPFTHVNPTSGMPVTQDQA
  857-  898 (57.02/28.49)	RII.YDTKEAIVSFLseNVDTCV...GEFLEEWARVSKMVVIAREV
  918-  945 (27.18/38.30)	QTVeFAYAENYTVSI..TCEDQISGTGGNF................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     245.45|      76|     341|     285|     385|       3
---------------------------------------------------------------------------
  174-  232 (71.04/26.45)	............................................................YLRLPTcikkyI..IQPTPLIDSEVRKTLSD..IEEA.IRY..RLRMWEV.IPVEMAQYRIADGRVY
  253-  357 (112.68/72.03)	GWffvhveflievgGDVATLQEF.............PRIP..tgllkRHitdeadarlayYLRLPE.....L..EQVPPGFEPPSRPKLPPGYVDAPLVRVFNFLQMMSLsYQLEILWYQAQRMRAL
  358-  434 (61.72/45.13)	GW............ADYLTVQMTqnrksmtvsywirPTAPsipgqqhRK............FKLPL.....LggKVIISIVETRGPPQAGPGPDRSPKMRTLAKLQ.....................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.97|      12|      22|     436|     447|       4
---------------------------------------------------------------------------
  436-  447 (20.84/ 9.83)	KAKLGDATPSDE
  461-  472 (22.13/10.84)	KGALGARVPPDQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      92.18|      22|      22|     590|     611|       5
---------------------------------------------------------------------------
  567-  588 (22.90/12.28)	.LAAAGRVsRFNVISEKIN..DDPT
  590-  611 (34.02/21.94)	LLNALFRL.RFMTIIDLAE..SKAR
  613-  636 (35.26/23.02)	LGLQCYRM.RHFPQQEWAKlgSDAR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.64|      16|      16|      32|      47|       6
---------------------------------------------------------------------------
   32-   47 (28.10/18.65)	DDVPL.ELLERELPVVY
   49-   65 (23.54/14.39)	DQIYLkELLQRVVQTIY
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP30143 with Med14 domain of Kingdom Fungi
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