<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP29810

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDAVIDARFERVEKALGALIDSISKYNPSISRVSDLVLADRELQDGLQNVQTHQNNVLRIQKLKATTAGLDAQIRSTLEVLASTRKDMTATPSTVFPDRLPNPISFEELLAFARRISKTTLPPPGVTNGVDLDGTGGDTTPGGGPAILTTGQDAGASTPAGPVPQTPTGPVSVAATPTPMSAVVNGGVGTPAPGTPSNTRQDSAMPDAQPQQGPQGGAANGGEVSAGNDKSVLPELFSQWMNPHTGAIFLPWPHEDQIRMGGLAANQSLSDRGIDPKGHDPAEEERLAAESAAEAKRLEEERVEAERKAMAEREARARQEMERRRAQQREMEQRDALLRGSAGSGAQSPTSASPTVKKQFQFTAFDDDDDSDNSD
Length375
PositionMiddle
OrganismGaeumannomyces graminis var. tritici (strain R3-111a-1) (Wheat and barley take-all root rot fungus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Magnaporthales> Magnaporthaceae> Gaeumannomyces.
Aromaticity0.03
Grand average of hydropathy-0.629
Instability index44.95
Isoelectric point4.85
Molecular weight39730.44
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364141

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP29810
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.73|      15|      15|     283|     297|       1
---------------------------------------------------------------------------
  283-  297 (24.72/16.08)	EEERLAAESAAEAKR
  299-  313 (26.01/17.26)	EEERVEAERKAMAER
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      72.89|      15|      15|     137|     151|       2
---------------------------------------------------------------------------
  137-  151 (30.48/13.61)	GDTTPG.GGPAILT.TG
  155-  169 (23.18/ 8.70)	GASTPA..GPVPQTpTG
  187-  200 (19.23/ 6.03)	GVGTPApGTPS..N.TR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.91|      17|      26|      79|     104|       3
---------------------------------------------------------------------------
   79-  103 (23.66/29.34)	EVLASTRKdmtatpstVFPDRLPNP
  108-  124 (30.24/12.72)	ELLAFARR........ISKTTLPPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.93|      16|      26|     203|     218|       4
---------------------------------------------------------------------------
  203-  218 (31.22/16.65)	SAMPDAQ..PQ.....QGPQGGA
  225-  247 (17.72/ 6.29)	SAGNDKSvlPElfsqwMNPHTGA
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP29810 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) RISKTTLPPPGVTNGVDLDGTGGDTTPGGGPAILTTGQDAGASTPAGPVPQTPTGPVSVAATPTPMSAVVNGGVGTPAPGTPSNTRQDSAMPDAQPQQGPQGGAANGGEVSAGNDKSVLPELFSQWMNPHTGAIFLPWPHEDQIRMGGLAANQSLSDRGIDPKGHDPAEEERLAAESAAEAKRLEEERVEAERKAMAEREARARQEMERRRAQQREMEQRDALLRGSAGSGAQSPTSASPTVKKQFQFTAFDDDDDSDNSD
115
375










Molecular Recognition Features






MoRF SequenceStartStop




1) MEQRDALLRGSA
2) QSPTSASPTVKKQFQFTAFDDDDDS
331
347
342
371