<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP29331

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMGDQAALSGGLRLIDVRQILDVFLEQTDSHLRDLSANLGAQGDEDRKRALLLFLHNTKQRLLRLLIVVKWANKARVVAEVGRILDVAARQANACRDAADQLAYLHGELEDTRVPAYDVPTALEELRPPPAPPPAVRRARLRLMDQILRSTLLQEKLPAGVTVASVGGGVTKLRCGDLWEARLTLDASAGGAEGEPVTSHDEPQKATGADSASKHASASEPQNADAEPMEVDGQQALNSAMEAEVAGASEESDGGRVQPGGVLDERFRWRVLSAKALPQFTVTALMAPQQSSQLQGAIEMRMWAALDAAEEVERREGPQKESASEKPESGSTEQGPSGSSLPGVKAETNAKPEAAGKSSAAQDMPRQAPLEILHGVLRDVGSRLVLNQAVIWARSATKGPNGRWAKLLRIEPAKTLSLGIRICYWLNVPTVTPEDLRRLQQPESAPPLPKPTQQPQQHTPPAIEIGVAKSGEPSIVARPELVHVIHSVAVRAAGAAVALSMDLASLDAEQVLLRAAQANAAIQLAAIEALLQRSGNLQANNGAIALQPPAADGVASGALPSPVLELSCDGEPLLSVSVQLRTGLLLLHSGDGGSGGFFSNLGQELAPLLRQEGERLAALQAEALKQVPAEGLGTVGAAAADRLARSIESLWLNLSVRSRYSQLLSAAQSLGLHRAQIPPALLKAYAASNPLARLPAKNVLVLAFPPAPPMPSPPDTLAGAEGASRQDRLLRLLGKGGAGVQLYLQAEMCRGLQWRYGLVFASCDARNTPVKVLQVLQVPVDLDLRPASGGQASIGAAQVGSTDKRSADNATSDAQTPSGQQPEQATARGGEENARENGHVEADGAAGTDARDQAQWGLRHAAELGEVVRWCAPRIAWELLRLQLKVLGISYTEEVCLAELPAAACALMANGQVPLKAKTSAATAASGALQKCLRLHGLHSLKLLDHTNGVSGPTGVLLRVFPDLQLGQSAWSVTVQSAYFGGLPASCAACSVQADADCGAQHISQTMHGLTLAYSFATGGTVERATGDLRRLIRLHLALARIAALMRAAAGTDSAALGDFQPSTNSSDKTAPSQRHDALANGNVAKHSPGISAPAQACNSSIDSKTGIASTAGHDSRFASLTWQAQQGSGVVSVSAVSADRAVLLITAPTDVAEASGNRTVVADGVSDAVPKQHSSLPQLRVTMEWVAVKGSSAARSSAGAEFGGGRMTAQEAGSNGIRCRMSSAPALLAPVLQAFADMADAGEEELLLDALSLCGCALATLAAELAPEALRNAGLLPEEVSMDAAVAPYKLRLCISQAPDAQLTVGMHLLASGLIWLAPSASQEHVRADLVDRLSRLPEAFLPDAPETAASDQESGESKRRGPQTAPSVLDSAMEIDGVASPPLEAPAGRSPAAAGLWVRVKTLPAALRMVLKVAAEMA
Length1419
PositionTail
OrganismCoccomyxa subellipsoidea (strain C-169) (Green microalga)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Chlorophyta> core chlorophytes> Trebouxiophyceae> Trebouxiophyceae incertae sedis> Elliptochloris clade> Coccomyxa.
Aromaticity0.03
Grand average of hydropathy-0.092
Instability index53.14
Isoelectric point5.83
Molecular weight148149.38
Publications
PubMed=22630137

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP29331
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     279.13|      70|      81|     518|     587|       1
---------------------------------------------------------------------------
  518-  585 (108.18/42.27)	.............NAAIQLAAIEALLQRS..GNLQANNGAIALQPPAADGVAS.GALPSPVLELSCDGEPLLSVSVQLRTGLLL
  586-  663 (82.37/30.50)	LHsgdggsggffsNLGQELA...PLLRQE..GERLAALQAEALKQVPAEGLGTvGAAAADRLARSIES.LWLNLSVRSRYSQLL
  671-  731 (88.58/33.33)	LH...........RAQIPPALLKAYAASNplARLPAKNVLVLAFPPAP.......PMPSPPDTLA..GAE..GASRQDRL.LRL
---------------------------------------------------------------------------
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     462.69|     122|     347|     801|     932|       2
---------------------------------------------------------------------------
  201-  355 (150.56/68.94)	EPQKATGADSASKHASASEPQNADAEPMEVDGQQalNSAMEAEV.........AGASEESD...GGR..VQPGGVLD.ERFRWrvlsakalpqftvtalMAPqqssqlqgaiemRM.WAAL.............DAAEEVERREGPQkeSASEKPESGsteQGPsgsslpgVKAETNAKPEAAG
  802-  926 (190.69/113.33)	DKRSADNATSDAQTPSGQQPEQATARGGEENARE..NGHVEADG.........AAGTDARD...QAQwgLRHAAELG.EVVRW................CAP............RIAWELLRLQL....KVLGiSYTEEVCLAELPA..AACALMANG...QVP.......LKAKTSAATAASG
 1187- 1258 (54.76/20.40)	......................................AVKGSS.........AARSSAGAefgGGR...MTAQEAGsNGIR.................CRM............SSAPALLAPVLqafaDMAD.AGEEELLLDALSL..CGCAL..............................
 1346- 1414 (66.67/26.77)	.PETA.ASDQESGESKRRGPQTA...PSVLD......SAMEIDGvasppleapAGRSPAAA...G..........L......W................VRV............KTLPAALRMVL....KV.....................................................
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     311.67|     100|     349|      24|     140|       3
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   24-  140 (148.14/132.64)	LEQTDSHLRDLSANL...........GAQGDEDRKRALllfLHNTKQRLLRLLI.VVKWANKARVVAEVGRILDVAarqanacrDAADQLAylhgelEDTRVPAYDVPTALEELRPPPAPPPAVRRARL
  369-  480 (163.53/107.01)	LEILHGVLRDVGSRLvlnqaviwarsATKGPNGRWAKL...LRIEPAKTLSLGIrICYWLNVPTVTPEDLRRLQQP........ESAPPLP......KPTQQPQQHTPPAIEIGVAKSGEPSIVARPEL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     168.90|      55|      74|    1040|    1101|       4
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 1040- 1101 (85.48/69.43)	RIAAL.MRAAAGTdsaalGDFQPSTNSSDK.....TAPSQRhdALANGNVAKHSPGISAPAQACNSSI
 1116- 1176 (83.43/51.09)	RFASLtWQAQQGS.....GVVSVSAVSADRavlliTAPTDV..AEASGNRTVVADGVSDAVPKQHSSL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.00|      26|     820|     159|     184|       6
---------------------------------------------------------------------------
  159-  184 (47.67/25.91)	GVTVAS..VGGGVTKLRCGDLWE.ARLTL
 1008- 1036 (36.33/18.06)	GLTLAYsfATGGTVERATGDLRRlIRLHL
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP29331 with Med14 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ALDAAEEVERREGPQKESASEKPESGSTEQGPSGSSLPGVKAETNAKPEAAGKSSAAQDMPRQAPLEIL
2) ALGDFQPSTNSSDKTAPSQRHDALANGNVAK
3) DLRRLQQPESAPPLPKPTQQPQQHTPPAIEI
4) LDASAGGAEGEPVTSHDEPQKATGADSASKHASASEPQNADAEPMEVDGQQALNSAMEAEVAGASEESDGGRVQPGGVLDE
5) LPDAPETAASDQESGESKRRGPQTAPSVLDSAM
6) QASIGAAQVGSTDKRSADNATSDAQTPSGQQPEQATARGGEENARENGHVEADGAAGTDARDQ
304
1055
434
184
1342
790
372
1085
464
264
1374
852










Molecular Recognition Features






MoRF SequenceStartStop




1) AGLWVRVKTLPAALRMVLKVAAEMA
2) FRWRV
3) VRRARLRL
1395
266
135
1419
270
142