<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP28893

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTTELDSSSKMLQSNGISNGLQDFNNQAKFDTPMTNSNQSNDPVSVPNSINKLPPSYTRLSLETNKPPTIPHVQINQTSLSLIIRNLTVFTIKEISQYMKTNLHNTMDNSKTTKKINFLNLIIFLRNQFLKLYVLIKWSRTIKNNNFHILIDLLNWFRTTNMSVNNCIWSLKSSLESMTNAKLPNVDLITALEVLSLGRPNLQTFKILKNDNLNYVNGMIEIPSNLIISKLNDLNLVNSIKISMMEVPPQFNNYNVKNGKIYITVPGEFEIQLSTVDHQSNLFFVDLTLLLNSSSETKESNDETTSDTGNNNSNDTSNMENNDDNKSSSPGNKIISKNFDNSLPLNKQRLEKVINEILFKSSKPLFSLYQFLHKYILTLKLYMIHMELLNLETLGKYSGGNLIHSYDSKKSIINGRYWINGKTGNKGKFTIGVAKSTETLVLRWDNYNEISTQDKTPSIYPDLLNNIENILDEIMFNHANLIRLDLLSKKVFQEDEDNLDVLLFQLPTTCQSTAPVQLKIDLITGVFYFKNPTPLLLEYTQQINRAETTTDLLKILQKLKLDKITQILNNMFEKTGWICSKAIKLSKSITTDIKVNNRTKNNEDDDRILQRDMFVSLPKWPIKWYLVLSIISSDSSCIIEKRIGKIVSNKGNWELKYLDPKSIHSSKMESITYQKIMPLQKSILHRIINHMLIDSLNQLNIHNKVCSTEVINSVLPKYIVEDLQKDSSDDSSEEYTSVIALELQSFLEGSKALNGILENSMFLRIDYKNSLIRLYAKFKKDKMIKQVQCEELLTHFIEDNSLDFYLNETFKNLGEIVKYLTTFRQKLMQLVVVTDVVDKLHKNFSSEFFKITELKPNEISFKYLKDQDTNDDKQDCTINIITSEQMVKNLTFKLSESNPQHVIQPFINNSSLDYHFIFNYLQFTSSFFITLSKIINETQQNTVKHSPTVRLGLHNLSEYQLIYYNPESGTKLTLIIELKNVSHNSRSKIHYFIHFSEDEHITTKSLAYPMIHQVSNQIFTLDANAQAKSRYPFAIRLIDGISCDSRDIENILSDIHEILKVDSDKKNNTN
Length1070
PositionTail
OrganismKazachstania africana (strain ATCC 22294 / BCRC 22015 / CBS 2517 / CECT 1963 / NBRC 1671 / NRRL Y-8276) (Yeast) (Kluyveromyces africanus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kazachstania.
Aromaticity0.08
Grand average of hydropathy-0.359
Instability index38.84
Isoelectric point7.86
Molecular weight123244.64
Publications
PubMed=22123960

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
TFIIB-class transcription factor binding	GO:0001093	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP28893
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.64|      11|      39|     517|     527|       1
---------------------------------------------------------------------------
  517-  527 (18.80/10.95)	QLKIDLITGVF
  558-  568 (17.84/10.01)	KLKLDKITQIL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      96.20|      34|      42|     753|     791|       2
---------------------------------------------------------------------------
  753-  787 (52.68/45.14)	LNGILE.NSM..FLRIDYKN..SLIRLYAKFKKdKMIKQV
  793-  831 (43.52/21.91)	LTHFIEdNSLdfYLNETFKNlgEIVKYLTTFRQ.KLMQLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.06|      27|      40|      74|     100|       3
---------------------------------------------------------------------------
   74-  100 (44.03/26.60)	QINQTSLSLIIRN..LTVFTIKEISQYMK
  115-  143 (40.03/23.50)	KINFLNLIIFLRNqfLKLYVLIKWSRTIK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     189.21|      60|     285|       1|      66|       4
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    1-   66 (90.06/62.45)	MTTELDSSSKMLQSNGisNGLQDFNNQAKFDTP.MTNsNQSNDPvSVPNsiNKLPPSYTRLSLETNK
  287-  347 (99.14/51.21)	LTLLLNSSSETKESND..ETTSDTGNNNSNDTSnMEN.NDDNKS.SSPG..NKIISKNFDNSLPLNK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.17|      18|      40|     211|     228|       5
---------------------------------------------------------------------------
  211-  228 (32.39/21.65)	DNLNYVNG..MIEIPSNLII
  252-  271 (28.77/18.34)	NNYNVKNGkiYITVPGEFEI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      87.14|      24|      32|     620|     643|       7
---------------------------------------------------------------------------
  620-  643 (43.88/25.85)	WPIKWYLVLSIISSDSSCIIEKRI
  653-  676 (43.25/25.38)	WELKYLDPKSIHSSKMESITYQKI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      64.88|      20|      39|     393|     412|       8
---------------------------------------------------------------------------
  393-  412 (34.60/19.89)	TLG.KYSGGNLIHSYDSKKSI
  430-  450 (30.27/16.56)	TIGvAKSTETLVLRWDNYNEI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.53|      14|      40|     920|     945|      11
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  920-  935 (19.67/ 7.81)	YLQFTSSFFITLskII
  963-  976 (23.85/16.03)	YYNPESGTKLTL..II
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      73.00|      24|     463|     576|     602|      15
---------------------------------------------------------------------------
  576-  602 (39.71/30.70)	GWIC.SKAIklsKSITTDI....KVNNRTKNN
 1040- 1068 (33.29/18.12)	GISCdSRDI...ENILSDIheilKVDSDKKNN
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP28893 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ETKESNDETTSDTGNNNSNDTSNMENNDDNKSSSPGNK
296
333










Molecular Recognition Features






MoRF SequenceStartStop




NANANA