<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP28893

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTTELDSSSKMLQSNGISNGLQDFNNQAKFDTPMTNSNQSNDPVSVPNSINKLPPSYTRLSLETNKPPTIPHVQINQTSLSLIIRNLTVFTIKEISQYMKTNLHNTMDNSKTTKKINFLNLIIFLRNQFLKLYVLIKWSRTIKNNNFHILIDLLNWFRTTNMSVNNCIWSLKSSLESMTNAKLPNVDLITALEVLSLGRPNLQTFKILKNDNLNYVNGMIEIPSNLIISKLNDLNLVNSIKISMMEVPPQFNNYNVKNGKIYITVPGEFEIQLSTVDHQSNLFFVDLTLLLNSSSETKESNDETTSDTGNNNSNDTSNMENNDDNKSSSPGNKIISKNFDNSLPLNKQRLEKVINEILFKSSKPLFSLYQFLHKYILTLKLYMIHMELLNLETLGKYSGGNLIHSYDSKKSIINGRYWINGKTGNKGKFTIGVAKSTETLVLRWDNYNEISTQDKTPSIYPDLLNNIENILDEIMFNHANLIRLDLLSKKVFQEDEDNLDVLLFQLPTTCQSTAPVQLKIDLITGVFYFKNPTPLLLEYTQQINRAETTTDLLKILQKLKLDKITQILNNMFEKTGWICSKAIKLSKSITTDIKVNNRTKNNEDDDRILQRDMFVSLPKWPIKWYLVLSIISSDSSCIIEKRIGKIVSNKGNWELKYLDPKSIHSSKMESITYQKIMPLQKSILHRIINHMLIDSLNQLNIHNKVCSTEVINSVLPKYIVEDLQKDSSDDSSEEYTSVIALELQSFLEGSKALNGILENSMFLRIDYKNSLIRLYAKFKKDKMIKQVQCEELLTHFIEDNSLDFYLNETFKNLGEIVKYLTTFRQKLMQLVVVTDVVDKLHKNFSSEFFKITELKPNEISFKYLKDQDTNDDKQDCTINIITSEQMVKNLTFKLSESNPQHVIQPFINNSSLDYHFIFNYLQFTSSFFITLSKIINETQQNTVKHSPTVRLGLHNLSEYQLIYYNPESGTKLTLIIELKNVSHNSRSKIHYFIHFSEDEHITTKSLAYPMIHQVSNQIFTLDANAQAKSRYPFAIRLIDGISCDSRDIENILSDIHEILKVDSDKKNNTN
Length1070
PositionTail
OrganismKazachstania africana (strain ATCC 22294 / BCRC 22015 / CBS 2517 / CECT 1963 / NBRC 1671 / NRRL Y-8276) (Yeast) (Kluyveromyces africanus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kazachstania.
Aromaticity0.08
Grand average of hydropathy-0.359
Instability index38.84
Isoelectric point7.86
Molecular weight123244.64
Publications
PubMed=22123960

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
TFIIB-class transcription factor binding	GO:0001093	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP28893
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.64|      11|      39|     517|     527|       1
---------------------------------------------------------------------------
  517-  527 (18.80/10.95)	QLKIDLITGVF
  558-  568 (17.84/10.01)	KLKLDKITQIL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      96.20|      34|      42|     753|     791|       2
---------------------------------------------------------------------------
  753-  787 (52.68/45.14)	LNGILE.NSM..FLRIDYKN..SLIRLYAKFKKdKMIKQV
  793-  831 (43.52/21.91)	LTHFIEdNSLdfYLNETFKNlgEIVKYLTTFRQ.KLMQLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.06|      27|      40|      74|     100|       3
---------------------------------------------------------------------------
   74-  100 (44.03/26.60)	QINQTSLSLIIRN..LTVFTIKEISQYMK
  115-  143 (40.03/23.50)	KINFLNLIIFLRNqfLKLYVLIKWSRTIK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     189.21|      60|     285|       1|      66|       4
---------------------------------------------------------------------------
    1-   66 (90.06/62.45)	MTTELDSSSKMLQSNGisNGLQDFNNQAKFDTP.MTNsNQSNDPvSVPNsiNKLPPSYTRLSLETNK
  287-  347 (99.14/51.21)	LTLLLNSSSETKESND..ETTSDTGNNNSNDTSnMEN.NDDNKS.SSPG..NKIISKNFDNSLPLNK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.17|      18|      40|     211|     228|       5
---------------------------------------------------------------------------
  211-  228 (32.39/21.65)	DNLNYVNG..MIEIPSNLII
  252-  271 (28.77/18.34)	NNYNVKNGkiYITVPGEFEI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      87.14|      24|      32|     620|     643|       7
---------------------------------------------------------------------------
  620-  643 (43.88/25.85)	WPIKWYLVLSIISSDSSCIIEKRI
  653-  676 (43.25/25.38)	WELKYLDPKSIHSSKMESITYQKI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      64.88|      20|      39|     393|     412|       8
---------------------------------------------------------------------------
  393-  412 (34.60/19.89)	TLG.KYSGGNLIHSYDSKKSI
  430-  450 (30.27/16.56)	TIGvAKSTETLVLRWDNYNEI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.53|      14|      40|     920|     945|      11
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  920-  935 (19.67/ 7.81)	YLQFTSSFFITLskII
  963-  976 (23.85/16.03)	YYNPESGTKLTL..II
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      73.00|      24|     463|     576|     602|      15
---------------------------------------------------------------------------
  576-  602 (39.71/30.70)	GWIC.SKAIklsKSITTDI....KVNNRTKNN
 1040- 1068 (33.29/18.12)	GISCdSRDI...ENILSDIheilKVDSDKKNN
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP28893 with Med14 domain of Kingdom Fungi
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