<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP28672

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMSRTFATFLYNFMRNGSPRFNNIELVHRMNGENTPRAGNINGEKREEDPMSNADSKSNGSDELLTKNGGKTGPPSIPHITNNIIPLSNILKYYSQTAYKQLTNIIDNLSNNQESESDLNRKKSFLQVIISMRQDFVKIYTLVKWASVSKDVSKLIDLLNWFRSQDFFFEQLGFGLNELNRYSGAKLPNSDIITALDVFIKKRPQLPSYNFVKTPPISPGKILEVFENLNLSLSARLALMDDLPPRFARHFEVQDGRIYFTIPNEFMVSVTAPNDLIIDSVDDYSRSPFYFIDFKFLFGINPDNTLISHKKNKIATSLPRSSFTRLEGIANSVLLTRGLQGLYDLLHRYAISFKLYLLYKQLKDLAINSRWRNNIKYKYDNSKSLLIINYWSNHHSSKNWKSFIELGIDKNYHLNYRWFKNGVYDTTNKFSISFGRSNQSEVNSDGTSDAQDHELPDDVAEDAGDEIIDLNIDTILTSVASKHAEIVMDHLFEKLKGSLASDLDNSCTRTSIHQIRIKLTPRKSAILSLNPLSGFFYFTDPSPIQNQAIKQMNFPSFNNNLGKAFFTEADMIDYCVSSIVQLRLHMYGKEIGNKLSTNEWINNDIISLNEYETAKLFNYSVNQLVGNPNSTLIYFYRCKNWPSSWFLVNMINGFHFSTFWWVARIKSIKGEWKIQWVRNLGFTEDSNQVADITLNYDFFFSLSNTCSNMILDHMILEQLQLRDIHFIKSDSRDKWLQALDKHGLSNVSSTLFDDQTSDSTYESMIILYNNNLIPVNNSSSSLFLLVRLSNLNSATQMGLCLFGKVRNIPNGLSDIADSSIKIKKNEEYFTIEDSINLSNEINDNYALENNLLGGIFLHLERLSKLIKILHQLHSNNSKVIENTINSIEVEFPNDLRSVTITLPENQDNIKLASRADESSEQKLIIDNINRYLEANKAKEGVIGALKYLQEVSSFIKTINAVRSHLDNKNKAKLASGLPKLSFEVAIRSVNLIQFVYYLSYNTGNSKKIFKDKVTINLSFRTNKFYKEKKLYVKLSLKENLHSKNLKHRKLFELVFKAIGSFKVPDGVSFIRLNSDFLIDGDSLEPLMVDITDCFIHYMNETN
Length1101
PositionTail
OrganismPichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) (Hybrid yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Debaryomycetaceae> Millerozyma.
Aromaticity0.11
Grand average of hydropathy-0.336
Instability index35.23
Isoelectric point8.40
Molecular weight126357.11
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP28672
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     129.49|      35|      55|     956|     990|       1
---------------------------------------------------------------------------
  303-  322 (22.04/ 7.79)	..N.TLISH...K.KNKIATSLPRSSF.........
  956-  990 (60.28/32.92)	TIN.AVRSHLDNKNKAKLASGLPKLSFEVAIRSVNL
 1013- 1044 (47.17/24.31)	TINlSFRTNKFYKEK.KL...YVKLSLKENLHSKNL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.75|      15|      15|     130|     144|       2
---------------------------------------------------------------------------
  130-  144 (27.51/16.27)	SMRQDFVKIYTLVKW
  146-  160 (25.24/14.38)	SVSKDVSKLIDLLNW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      71.19|      15|      15|     380|     394|       3
---------------------------------------------------------------------------
  362-  374 (16.81/ 7.02)	..KDLAINSRWRNNI
  380-  394 (27.94/17.35)	NSKSLLIINYWSNHH
  398-  412 (26.44/15.96)	NWKSFIELGIDKNYH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      75.20|      23|      86|     840|     866|       4
---------------------------------------------------------------------------
  768-  790 (39.58/ 6.36)	NNNLIPVNNSSSSLFL.LVRLSNL
  841-  864 (35.63/26.55)	NDNYALENNLLGGIFLhLERLSKL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     141.15|      46|     245|     231|     292|       6
---------------------------------------------------------------------------
  232-  292 (59.76/77.25)	LSARLAlmDDLPPRFARHfEVQDGRIYFTiPNEFMvsvtapndliIDSVDDYSrSPFYFID
  494-  539 (81.39/49.31)	LKGSLA..SDLDNSCTRT.SIHQIRIKLT.PRKSA..........ILSLNPLS.GFFYFTD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.41|      13|      80|     339|     352|       7
---------------------------------------------------------------------------
  340-  352 (24.85/15.13)	GLYDLLHRYAISF
  421-  433 (23.56/ 9.02)	GVYDTTNKFSISF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     191.79|      53|     434|     435|     490|       9
---------------------------------------------------------------------------
  435-  490 (78.42/60.06)	RSNQSEVnSDGTSDAQDHELPDDVAEdaGDEIIDL..NIDTI.LTSVASKHAE..IVMDHL
  683-  713 (43.92/23.97)	..................EDSNQVAD......ITL..NYD.F.FFSLSNTCSN..MILDHM
  872-  927 (69.45/42.86)	HSNNSKV.IENTINSIEVEFPNDL....RSVTITLpeNQDNIkLASRADESSEqkLIIDNI
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP28672 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) NNIELVHRMNGENTPRAGNINGEKREEDPMSNADSKSNGSDELLTKNGGKTG
21
72










Molecular Recognition Features






MoRF SequenceStartStop




1) TFATF
4
8