<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP28286

Description Mediator of RNA polymerase II transcription subunit 1
SequenceEAEKMTKMSSLLERLHAKFNQNRPWSETFKLIRQVTEKRVVMSTGGHQYLVSCLETLQKALKVTSLPAMTDRLESIARQNALGSHLSANGTECYITSDMFYVEVQLDPTGHLSDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYKLPGDNKLKTKMYLALQSLEQDLSKMALMYWKATNASPLDKILHGSVGYLTPRSGGHLMNLKYYASPYDLLEDKTATPILLHENNVPRTLGMNASVTIEGTPSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPRPIPVSRAFVQKLQNCTGIPLFDTQPIYVPLYELITQFELSKDPDPVPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLIEKVTFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGILQFEVCPLSDTCFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSMNMKERHESVGHGEDFSKVSQNPILTSLLQITGNVGSSIGSSPTPPHHTPPPVSSPASNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMGSGANKTKKKKSSRVLPEKPKHQTEDDFQRELFSMDVDSQNPVFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHPQPSIQRMVRLSSSDSIGADVTDILSDIAEEASKLPSTSDDCPPIDTPVRDSSSSAHSQSALFDPDVFQNNNNENPYTDPADLIADATGSPNSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPVLAGDSVENKFKGNSQADTVDFSIIAVASKALGPADIMEHHSGSQSPLLSTGDLGKDKTQKRIKDGNGTGCSSLSGPSLDSKPGKRSRTPSSDGKSKDKPPKRKKADTDGKSPSHSSSNRPFTPPASTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVAKPSSHSQYTSSGSMSSSGSKSHHGHSSSSSSSTSTSGKMKSSKSEGSSGSKMSSSMYSSQGCSGSSQSKSSSQSVGKPGSSPITKHGLSSSSGSNKVKPQGKPSSLMNPSLSKPNISPSHSRPSGSSDKLASPMKPVPGTPPSSKAKSPISSGSSGSHMSGTSSGSGMKSSGLGSSGSLSQKTPPSSNSCTASSSSFSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMEGQMGVSANSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSVSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRERDRDKDRDKKKSHSIKSESWSKSPISSDQSMSMTSNTILQDRPTMLTPDFVIGDEEDELMDAALLGN
Length1573
PositionMiddle
OrganismSarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Metatheria> Dasyuromorphia> Dasyuridae> Sarcophilus.
Aromaticity0.05
Grand average of hydropathy-0.662
Instability index54.23
Isoelectric point8.83
Molecular weight168111.10
Publications
PubMed=21709235

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:Ensembl
nucleolus	GO:0005730	IEA:Ensembl
nucleoplasm	GO:0005654	IEA:Ensembl

GO - Biological Function
DNA binding	GO:0003677	IEA:Ensembl
estrogen receptor binding	GO:0030331	IEA:Ensembl
LBD domain binding	GO:0050693	IEA:Ensembl
nuclear receptor coactivator activity	GO:0030374	IEA:Ensembl
peroxisome proliferator activated receptor binding	GO:0042975	IEA:Ensembl
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IEA:Ensembl
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
transcription corepressor activity	GO:0003714	IEA:Ensembl
vitamin D receptor binding	GO:0042809	IEA:Ensembl

GO - Biological Process
androgen biosynthetic process	GO:0006702	IEA:Ensembl
angiogenesis	GO:0001525	IEA:Ensembl
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	IEA:Ensembl
cellular response to epidermal growth factor stimulus	GO:0071364	IEA:Ensembl
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to thyroid hormone stimulus	GO:0097067	IEA:Ensembl
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
fat cell differentiation	GO:0045444	IEA:Ensembl
intracellular steroid hormone receptor signaling pathway	GO:0030518	IEA:Ensembl
keratinocyte differentiation	GO:0030216	IEA:Ensembl
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	IEA:Ensembl
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	IEA:Ensembl
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	IEA:Ensembl
negative regulation of apoptotic process	GO:0043066	IEA:Ensembl
negative regulation of keratinocyte proliferation	GO:0010839	IEA:Ensembl
negative regulation of neuron differentiation	GO:0045665	IEA:Ensembl
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:Ensembl
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IEA:Ensembl
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	IEA:Ensembl
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	IEA:Ensembl
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	IEA:Ensembl
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP28286
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
            11|     956.05|     104|     104|    1104|    1207|       3
---------------------------------------------------------------------------
  537-  605 (49.49/10.15)	....................................SSPGYG.....mTT........G..S...NP..MS.......GTT.......TPTNTF.PGGPITTL.......FNM.........................SM...NMKE.RHeSVGH.GedfskvSQNPILTSLLQ.....IT.G
  608-  718 (80.60/21.94)	..........GSSiGSSPTPPHHTPPPVS.SPASNTKNHPMLmnllkdNPaqdfstlyG..S...SP..LE...RQNSSSGsprmemgSGANKTKK..KKSSR.......VLP.........................EKPKHQTED.DF.QREL.F......SMD..VDSQNP.....VF.D
  728-  824 (90.36/25.64)	TPHITPAPSQCSTpPTTYPQPIPHPQPSIQRMVRLSSSDSIG......AD........V..TdilSD..IA...EEASKLP.......STSDDCPPIDTP.......................................VRDSS.SS.AH.SQSAlF......DPDVFQNNNN..........
  859-  952 (92.46/26.44)	NPDLLNSQSQSGF.GEEYFDE..SSQSGDNDDFKGFASQALN......TL........G......VP..V.......LAGD.......SVENKFKGNSQADTVdfsiiavASK.........................ALGPADIME.HH....S.G......SQS.......P.....LL.S
  953- 1063 (85.44/23.78)	TGDLGKDKTQ.....KRIKDG..NGTGC..SSLSGPSLDS........KP........G..K...RS..RT.......PSS.......DGKSKDKP................PkrkkadtdgkspshsssnrpftppaSTGGSK.SP.GS.SGRS.Q......TPPGVATPPIPkitiqIPkG
 1104- 1207 (180.70/59.90)	SGKMKSSKSEGSS.GSKMSSSMYSSQGCSGSSQSKSSSQSVG......KP........G..S...SP..IT...KHGLSSS.......SGSNKVKPQGKPSSL.......MNP.........................SLSKPNISP.SH.SRPS.G......SSDKLASPMKP.....VP.G
 1211- 1261 (64.32/15.77)	SSKAKSPISSGSS.GSHMSGT.........SSGSGMKSS.....................................GL...................G..SS..........G.........................SLSQK..TP......PS.S......NSCT.AS.............
 1262- 1338 (74.55/19.65)	.......SSSFSS.GSSMSSS....QNQHGSSKGKSPSRN.K......KP........S..L...TA..VIdklKHGV.VT.......SG.....PGGE............DP.........................MEGQMGVSAnSS.SHPM.S......SKHNMS..............
 1339- 1411 (79.40/21.49)	GGEFQ.GKREKSD.KDK..SKVSVSGGSVDSSKKTSESKNVG......ST........G..V...AKiiIS...KHDGGSP.......SIKAKV............................................TLQKP...........G.E......SSGE...............G
 1413- 1484 (98.64/28.78)	RPQMASSKN.............YGSPLIS.............................G..S...TP..KH...ERGSPSH.......SKS....PAYTPQNL.......DSE.........................SESGSSIAE.KS.YQNS.P......SSDDGIRPL.P.....EY.S
 1485- 1554 (60.10/14.17)	TEKHKKHKKE....KKKVKDKDRDRERDRDKDRDKKKSHSIK......SE........SwsK...SP..IS...S.DQSMS.......MTSNTI.LQDRPTML........TP......................................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     122.12|      37|      48|     138|     175|       4
---------------------------------------------------------------------------
  138-  175 (59.92/42.16)	NFDEFSKHLKGLVNlYKLP..GDNKLKTKMYLALQSLEQD
  188-  226 (62.19/39.39)	NASPLDKILHGSVG.YLTPrsGGHLMNLKYYASPYDLLED
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     209.64|      50|      53|     391|     443|       5
---------------------------------------------------------------------------
  315-  363 (57.93/30.19)	........QNCTGIPLFDTQPIYVPLYelitqFelsKDPDPVPLNHNM.RF...YAALPGQ
  366-  416 (78.90/44.92)	CY...FLNKDAP..LP.DGRSLQGTLI.ekvtF...QHPGRVPLILNLIRHQVAYNTLIGS
  417-  469 (72.82/47.75)	CVkrtILKEDSPGILQFEVCPLSDTCF..svsF...QHPVNDSLV..CVVMDVQDSTHV.S
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP28286 with Med1 domain of Kingdom Metazoa
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