<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP27965

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMENGVQNGAHSNHDRDVKVNGGAVAVDSPAALDKGKGAAAGTSDAAAQNGHGDKTVPPANAQDAQRGTSTVSLPNRSRMNDLPDEIQHITQGFIPLSTIILRLAQATHDDLEKAIMDAARIKYSPPAINGSATGGGDPPDDSSEANKARKLTLLKFAQDAHTKWVKALVITDWSRKASTVSKLIDLKAHLDKKRSLFDFTLTELIELKRSLGQARLPRPDLKTALQILTTGEAPWIPEPGYIPPPSLTPEEQLKWIEELNTLLSLRLNLDDYDKIPHQFKNYTIKSGRVTFTVKGEFEVDLTIADEDFEKQFWFIDFRFDFTPSSERLSDALVAYLEGQVNDVLSREGLEGCYRFLHEFVLTHKINELKRQAIDLVRNSWTGNLKVEPLNRALAIQYWTSRYGPQAPKSWVMVAVQSGKQKNGLPDPKHPSHLVARWYRDNKEIKDVKLHLNIQEISAEALLKSAIAKHVEHILSSIHDKLAAAPRFVNRQAYMRLDISRSEPLDSSLEMQLGGKKMVKLLIEPITGFAAIQPHTRYGLQGETRINHGGKDPAEDGVACLENIRWGYVVDEFNRRGRSCGWAMTKIPVGNEEVKRLIKTREPFQPVFFQRQGLGQDWFVLMSLSLAGDEWWLIEVTRNGASRTVTTSIQLPFSKGQPELNDKFWNNLTSLAAAMISQAIELGELKKNRIDHTAKDMQSRCLTQQIRIPVIFIRLAHILDSPDSLAQTRAQDRRVQSSAPAWPADTVEVHFRGTKGSSAANSAGNDTAGLGISVDAVLKVKDKHKFKQLQARIDKNVAFDRRTGQFVFHIRGSVGKPVLQALADHIKSIDRLVGFLDAMRCAKGAVQCTEVGLGKVKFSYSDIVLPGETGEPRQWTAVLNMSRNGTRLILEPDNPHLRILDLLTKVVNTEKGLRVMMAYMTDVLPILRVFDTLEKKWEEVEAAGQGHIELLTPAADWFAVRYSLPSTAGPARQLTIDVWSKERQAQPYWFASRKQRGPPSPADDEFKAVLKGVWDSRGENWRGLTTGAVAQMGMGVFEMIQAMDDAVRAFATGNGPADDNASTAGLPTQVSQVFSSQGSGNGTPQTSQAQSQKQASQASQGQQPQRPRPSQTPNQNQMRKPSFGKQGGGAKGPVGGKNAPVVVLD
Length1144
PositionTail
OrganismVerticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) (Verticillium wilt)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Glomerellales> Plectosphaerellaceae> Verticillium.
Aromaticity0.07
Grand average of hydropathy-0.438
Instability index39.94
Isoelectric point9.08
Molecular weight126533.28
Publications
PubMed=21829347

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats
>MDP27965
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      99.96|      24|      25|    1075|    1098|       1
---------------------------------------------------------------------------
 1052- 1073 (21.81/ 8.35)	....GNG.PaddNASTAGLPTQVSQVF
 1075- 1096 (34.93/18.17)	SQGSGNGTP...QTSQAQSQKQASQ..
 1098- 1121 (43.22/24.38)	SQGQQPQRP...RPSQTPNQNQMRKPS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.21|      17|      25|     976|     996|       2
---------------------------------------------------------------------------
  976-  996 (23.97/25.63)	DvwsKERQA..QPYWfASRKQ..RG
 1002- 1022 (23.24/10.54)	D...DEFKAvlKGVW.DSRGEnwRG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.87|      19|      25|     886|     910|       3
---------------------------------------------------------------------------
  886-  904 (33.53/29.01)	RLILE..PDN.PHLRILDLLTK
  913-  934 (24.34/ 7.82)	RVMMAymTDVlPILRVFDTLEK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     143.89|      29|      29|      29|      57|       4
---------------------------------------------------------------------------
    1-   29 (29.00/14.40)	..MENGvqNGAHSNHDRDVKVNGGAVAVDSP
   30-   58 (46.61/27.89)	AALDKG..KGAAAGTSDAAAQNGHGDKTVPP
  114-  139 (38.58/21.73)	AIMDAA..RIKYSPPAINGSATGGGD...PP
  140-  165 (29.70/14.93)	DDSSEA..NKARKLTLLKFAQDAH.TKWV..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.03|      10|      33|     572|     581|       5
---------------------------------------------------------------------------
  572-  581 (22.06/13.66)	FNRRGRSCGW
  608-  617 (20.96/12.58)	FQRQGLGQDW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.77|      12|      25|     216|     227|       6
---------------------------------------------------------------------------
  216-  227 (20.27/11.81)	LPRPDLKTALQI
  242-  253 (22.50/14.07)	IPPPSLTPEEQL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.04|      16|      19|     179|     194|       7
---------------------------------------------------------------------------
  179-  194 (26.53/14.87)	TVSKLIDLKAHLDKKR
  200-  215 (25.52/14.06)	TLTELIELKRSLGQAR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      72.79|      22|      32|     788|     810|       8
---------------------------------------------------------------------------
  788-  810 (34.98/29.97)	LQARIDKNVAFDRRTGqFVFHIR
  818-  839 (37.81/26.87)	LQALADHIKSIDRLVG.FLDAMR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      83.51|      26|      32|     267|     296|       9
---------------------------------------------------------------------------
  267-  296 (34.34/35.69)	LNLDDYDkIPHQFknYTIkSGRVTFTVKGE
  301-  326 (49.17/31.40)	LTIADED.FEKQF..WFI.DFRFDFTPSSE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.14|      14|      25|     336|     350|      14
---------------------------------------------------------------------------
  336-  350 (18.28/17.87)	LEGQVNDvLSREGLE
  361-  374 (22.86/16.45)	LTHKINE.LKRQAID
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.58|      16|      25|     523|     538|      17
---------------------------------------------------------------------------
  523-  538 (29.54/21.94)	EPITGFAAIQPHTRYG
  551-  566 (30.04/22.46)	DPAEDGVACLENIRWG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.80|      10|      24|     736|     745|      18
---------------------------------------------------------------------------
  736-  745 (19.76/12.22)	SSAPAWPADT
  757-  766 (17.04/ 9.37)	SAANSAGNDT
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP27965 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DAVRAFATGNGPADDNASTAGLPTQVSQVFSSQGSGNGTPQTSQAQSQKQASQASQGQQPQRPRPSQTPNQNQMRKPSFGKQGGGAKGPVGGKNAPVVVLD
2) MENGVQNGAHSNHDRDVKVNGGAVAVDSPAALDKGKGAAAGTSDAAAQNGHGDKTVPPANAQDAQRGTSTVSLPNRSRMNDLPDEIQ
1044
1
1144
87










Molecular Recognition Features






MoRF SequenceStartStop




1) QMRKPSFGKQGGGAK
1116
1130