<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP27918

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMASLNMSPEELKQLELVRNRFAQLTSSLTSLRASVLNSNPLPSRESLQASVTILQQNIRSIQELADENADLFQRLAIHPSTNFPGRTHEHILTQLLRKKLEPDVESWVEEARATARAAGVDASKLAAGVRPRGVHDYDDEDAYDLGAAVNPDDGPSDPFSEQWADMADNFRQTLQQYVTVQVKKKYTVEEQAMGIENVRTGLRQTLEESDEEDEDEEEEEEEEEEEEGAAGTTAAQAAPGAPAAAGAAGATGATGAAEGQGGHGGGLPVVVEPEHIFYLQAQGSFDLPRNIPLESKRVQTGATRRVAPPR
Length310
PositionHead
OrganismMyceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) (Sporotrichum thermophile)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Sordariales> Chaetomiaceae> Thermothelomyces.
Aromaticity0.05
Grand average of hydropathy-0.638
Instability index64.74
Isoelectric point4.54
Molecular weight33621.45
Publications
PubMed=21964414

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP27918
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.06|      13|      15|      27|      39|       1
---------------------------------------------------------------------------
   27-   39 (22.06/15.97)	SLTSLRASV..LNSN
   43-   57 (17.99/11.86)	SRESLQASVtiLQQN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.81|      16|      18|     121|     136|       2
---------------------------------------------------------------------------
  121-  136 (29.46/16.93)	DASKLAAGVRP.RGVHD
  141-  157 (26.35/14.48)	DAYDLGAAVNPdDGPSD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP27918 with Med8 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AGVRPRGVHDYDDEDAYDLGAAVNPDDGPSDPFSEQWADMAD
2) MGIENVRTGLRQTLEESDEEDEDEEEEEEEEEEEEGAAGTTAAQAAPGAPAAAGAAGATGATGAAEGQGGHGGGLPVVVEP
127
193
168
273










Molecular Recognition Features






MoRF SequenceStartStop




1) DAYDLG
2) IFYLQAQ
3) LPRNIPLESKRVQTGATRRVAPP
4) PAAAGAA
141
276
287
242
146
282
309
248