<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP27707

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMADTALIHDLRPPTASARKRKQLRQGTPTKRGRVFIVVAMQREEKQLELSLEALISQVADLKNSLVNFIYKLENEYDRLTWPSVLDNFALLSGQLNTLNKVLKHEKTPLLRNQVIIPLVLSPDRDEELMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLSTDAARIGADVAQKQIQSLNKMCSNLLEKINKEDRESESGGIRQNKQTFNPADTNALVAAVAFGKGLSNRRPPGVTGPVQSGQPGANSIIAGGSAMQQVQMPGAPGQTQPMLGGVQMPQGGQPGKMPSGIKTNIKSASMHPYQR
Length307
PositionHead
OrganismAnolis carolinensis (Green anole) (American chameleon)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Lepidosauria> Squamata> Bifurcata> Unidentata> Episquamata> Toxicofera> Iguania> Dactyloidae> Anolis.
Aromaticity0.03
Grand average of hydropathy-0.563
Instability index48.16
Isoelectric point9.47
Molecular weight33743.20
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364144

GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IBA:GO_Central

GO - Biological Function
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IBA:GO_Central
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP27707
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.00|      12|      17|     259|     270|       1
---------------------------------------------------------------------------
  259-  270 (25.84/12.97)	MQQVQMP..GAPGQ
  275-  288 (21.17/ 9.45)	LGGVQMPqgGQPGK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      72.64|      17|      17|      90|     106|       2
---------------------------------------------------------------------------
   90-  106 (25.84/13.84)	LLSGQLNTLNKVLKHEK
  109-  124 (24.64/12.92)	LLRNQV.IIPLVLSPDR
  127-  142 (22.16/11.04)	ELMRQTEGRVPVFSHE.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.50|      18|      30|     204|     222|       3
---------------------------------------------------------------------------
  204-  222 (24.70/18.70)	GIRQNKQTFNPAdTNALVA
  237-  254 (29.80/17.42)	GVTGPVQSGQPG.ANSIIA
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP27707 with Med8 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) CSNLLEKINKEDRESESGGIRQNKQTFNPADTNALVAAVAFGKGLSNRRPPGVTGPVQSGQPGANSIIAGGSAMQQVQMPGAPGQTQPMLGGVQMPQGGQPGKMPSGIKTNIKSASMHPYQR
2) FSHEVVPDHLRTKPDPEVEEQEKQLSTDAARI
186
139
307
170










Molecular Recognition Features






MoRF SequenceStartStop




1) KMPSGIKTNIKSASMHPYQR
2) RKRKQLR
288
18
307
24