<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP27666

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTTEVDSSKMLNGKSFANSGSSSEPLQNDTTDTHSPILNGKMSQGATQENPQLKSPIPSTTNVTYTADDDIIQNQGLVPPPIPHVETNQVSLALIVRNLTIFTIKEISQYMKTNIHVNPSEGSSTKKLAFLKLIIFLRNQFLKLYVLIKWCKTIKNNNFHVMIDLLNWFRTTTMAVNGCIWALRGNLTSMTNAKLPNVDLVSALEVLSLGRTNLPSHNFKLSAEMDPLVKIPPDLILKKLKDLNLMVSIKVAMMDLPRQFHNYYIANGRIYIKVENEFEIQLSTIDRHSPLFFVDVNLLLSFNEKEIILPLNKQRLEKLINEILFKSNKPLFALYDFLHKYVITLQLYSLHLELITLETNGKFSGGNLVHSYDPKKCIISIRYWLNSKMGNKGKIMIGIGRESENLILKWDNKNAINNKRMTTVYNNIVTNLEAIFDEIMFNHCQLIRSDLLSKGVFQEDEEDQDVLLFEIPTTCLTMAAVQLKIDLINGIFYFKNPTPLLSTYVQQINRADSIEDLTRVLQKLKLDKIISILRNMFEKTGWVCSKVIRLEKRIKTTLNDIDSKSLLQQDLFICLPNWPVNWYLILSVISSKSSCMVEKRIGKVVSLKGKWELTYLDNTNTSTAKLETITYQKVVSLQKSILHRIVNHMLIDSLNQLKIRNRICPSDSSNSLLPECIINDSTNMPTKDSPTSATKQESEGTTLSEADTAALVGDPGDAQVNTGGECTSVIALELESFLEGSKALSSILESSMFLRIDYTHSEIRLYGRFKRNTMLIQCKCDDLLIHFIPDGSLTFFFSENFTTLSLIVQYLTSFRQKLMQLVVLTDVVERLHKNFASEYFKIVSLKVNEISFKYLKNSQDEQDCIINIITKKQMVKNLTVQLAASNPQHIIQPFIDSGRLDYHFIFNYLQFTSTLFATLKHILTESNPNKNEDGSIRYTTVNLGLHNLNEYQLVYCNREAGTKITLYIELKNVTHNTGKSLQFYIHFSKDEHITTKSRAYPMVHQVCNQVFMLDPKLISMDKSNSDMKNECKYPDAIKLVNGISCNSEDIEPILMDIHDILKVDSNVGNNDNGSELLPTL
Length1080
PositionTail
OrganismNaumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Naumovozyma.
Aromaticity0.08
Grand average of hydropathy-0.150
Instability index33.86
Isoelectric point7.75
Molecular weight123021.85
Publications
PubMed=22123960

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
TFIIB-class transcription factor binding	GO:0001093	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP27666
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      93.61|      30|     187|     792|     868|       1
---------------------------------------------------------------------------
  833-  868 (38.33/90.47)	KNfASEYFKIVSLkVNEISFkylkNSQDEQDCIINI
 1028- 1057 (55.28/17.53)	KN.ECKYPDAIKL.VNGISC....NSEDIEPILMDI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     176.85|      56|     198|     383|     439|       2
---------------------------------------------------------------------------
  383-  439 (94.39/60.24)	YWLNSKMGNKGKIMIG..IGReSENLILKW.....DNKNAINNKRMTTVYNNIVTNLEAIFDEI
  583-  645 (82.46/48.24)	YLILSVISSKSSCMVEkrIGK.VVSLKGKWeltylDNTNTSTAKLETITYQKVVSLQKSILHRI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.76|      14|     141|     922|     935|       3
---------------------------------------------------------------------------
  922-  935 (25.76/14.35)	IL.TESNPNKNEDGS
 1060- 1074 (21.00/10.34)	ILkVDSNVGNNDNGS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      44.78|      14|     187|     792|     805|       6
---------------------------------------------------------------------------
  792-  805 (26.89/15.85)	SLTFF..FS..ENFTTLS
  980-  997 (17.88/ 8.20)	SLQFYihFSkdEHITTKS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     213.55|      72|     390|      68|     150|      10
---------------------------------------------------------------------------
   68-  150 (104.23/100.36)	DDDIIqnqgL..VPPPIPHVETNQVSLALIvrNlTIFTIKEISQYMKTNI.HVNPSEGSST.....KKLAFLKLIIFLRNQFLKlyvlIKW
  463-  542 (109.32/73.93)	DQDVL....LfeIPTTCLTMAAVQLKIDLI..N.GIFYFKNPTPLLSTYVqQINRADSIEDltrvlQKLKLDKIISILRNMFEK....TGW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.14|      24|     252|      12|      36|      12
---------------------------------------------------------------------------
   12-   36 (38.33/26.45)	NGKSFANSGSSSEpLQNDTTDTHSP
  267-  290 (42.81/24.59)	NGRIYIKVENEFE.IQLSTIDRHSP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP27666 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MTTEVDSSKMLNGKSFANSGSSSEPLQNDTTDTHSPILNGKMSQGATQENPQLKSPIPST
1
60










Molecular Recognition Features






MoRF SequenceStartStop




NANANA