<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP27332

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTSSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQTCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLAVPMLGGDNGEAKFKGNSQADTVDFSIIAVAGKALGPADLMEHHSGSQSPLLTAGDLGKEKTQKRVKEGNGTSNNSLSGPGLDSKPGKRSRTPSNDGKSKDKPTKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSIYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSSSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPAGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSSSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGEGSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN
Length1581
PositionMiddle
OrganismEquus caballus (Horse)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Perissodactyla> Equidae> Equus.
Aromaticity0.04
Grand average of hydropathy-0.678
Instability index53.95
Isoelectric point8.88
Molecular weight168410.13
Publications
PubMed=19892987

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	ISS:UniProtKB
nucleolus	GO:0005730	ISS:UniProtKB
nucleoplasm	GO:0005654	IEA:Ensembl
nucleus	GO:0005634	ISS:UniProtKB

GO - Biological Function
chromatin binding	GO:0003682	ISS:UniProtKB
DNA binding	GO:0003677	IEA:Ensembl
estrogen receptor binding	GO:0030331	IEA:Ensembl
LBD domain binding	GO:0050693	IEA:Ensembl
nuclear receptor coactivator activity	GO:0030374	ISS:UniProtKB
peroxisome proliferator activated receptor binding	GO:0042975	IEA:Ensembl
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IBA:GO_Central
thyroid hormone receptor binding	GO:0046966	ISS:UniProtKB
transcription coactivator activity	GO:0003713	ISS:UniProtKB
transcription coregulator activity	GO:0003712	ISS:UniProtKB
transcription corepressor activity	GO:0003714	ISS:UniProtKB
vitamin D receptor binding	GO:0042809	IBA:GO_Central

GO - Biological Process
androgen biosynthetic process	GO:0006702	ISS:UniProtKB
angiogenesis	GO:0001525	ISS:UniProtKB
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	ISS:UniProtKB
cellular response to epidermal growth factor stimulus	GO:0071364	ISS:UniProtKB
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to thyroid hormone stimulus	GO:0097067	IBA:GO_Central
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
erythrocyte development	GO:0048821	ISS:UniProtKB
fat cell differentiation	GO:0045444	IEA:Ensembl
intracellular steroid hormone receptor signaling pathway	GO:0030518	ISS:UniProtKB
keratinocyte differentiation	GO:0030216	ISS:UniProtKB
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	ISS:UniProtKB
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	ISS:UniProtKB
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	ISS:UniProtKB
negative regulation of apoptotic process	GO:0043066	ISS:UniProtKB
negative regulation of keratinocyte proliferation	GO:0010839	ISS:UniProtKB
negative regulation of neuron differentiation	GO:0045665	ISS:UniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	ISS:UniProtKB
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IEA:Ensembl
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	ISS:UniProtKB
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	ISS:UniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	ISS:UniProtKB
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	ISS:UniProtKB
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of RNA biosynthetic process	GO:2001141	ISS:UniProtKB
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	ISS:UniProtKB
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP27332
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     441.01|      50|      50|    1081|    1130|       1
---------------------------------------------------------------------------
 1032- 1080 (58.37/14.19)	TPPTS..TGGSKSPGS..SGRSQTPPG....VA.......TPPIPKITIQIPKGTVMVG..K.PSSH
 1081- 1130 (81.82/23.25)	SQYTS..SGSVSSSGSK.SHHSHSSSS....SS.......SSASNSGKMKSSKSEGSSS..S.KLSS
 1131- 1181 (51.32/11.46)	SIYSS..QGSSGSSQSKnSSQSGGKPG....SSpitkhglSSSSSSTKMK...PQG.......KPSS
 1184- 1224 (55.89/13.23)	NPSLS..KPNISPS......HSRPAGG....SD.......KLASP...MKPVPG.TPPS..S.KAKS
 1225- 1271 (41.39/ 7.62)	.........PISSGSGG.SHMSGTSSStgmkSS.......SGLGSSGSL.SQKTPPSSN..ScTASS
 1272- 1313 (56.00/13.27)	SSFSS..SGSSMSSSQN.QH......G....SS.......KGKSPSRNKKPSLT..AVI..D.KLKH
 1314- 1367 (46.33/ 9.53)	GVVTSgpGGEDPMDGQM.GVSTNSSHP....MS.......SKHNMSGGEFQGKREKSDKdkS.KVSS
 1430- 1475 (49.89/10.91)	....N..YGSPLISGST.PKHERGSPS....HS.......KSPAYTPQNLDSESESGSS..I.AEKS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     254.98|      50|      50|     324|     373|       2
---------------------------------------------------------------------------
  298-  322 (25.16/ 9.83)	..........................DLPACFFL.KFPQPIPVSRA.................F...VQKL...Q..
  324-  373 (94.91/61.20)	CTGIPLF........ETQPTYVPLY.ELITQFELSKDPDPIPLNHNM...............RF...YAALPGQQHC
  383-  424 (35.58/17.50)	..................PDGRSLQgTLVSKITF.QHPGRVPLILNL..............iRHqvaYNTLIGS..C
  434-  489 (53.53/30.73)	SPGLLQF........EV....CPLS.E..SRFSVSFQH...PVNDSLvcvvmdvqdsthvscKL...YKGLSDALIC
  501-  547 (45.80/25.03)	CMSIPVTmrairrkaETIQADTPAL.SLIA..ETVED....MVKKNL...............P....PASSPG....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.57|      16|      21|     736|     756|       3
---------------------------------------------------------------------------
  734-  751 (25.59/ 8.93)	DTPHitPAPS.....QCSTPPTT
  755-  775 (17.98/ 6.73)	PVPH..PQPSiqrmvRLSSSDSI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     242.86|      50|      64|     549|     603|       5
---------------------------------------------------------------------------
  549-  599 (89.38/36.30)	GMTTGNNPM....SGTTTPTNT.FPGGPI......TTLFNMSMS.I...KDRHESvGHGED.FSK......V.S
  615-  660 (57.15/20.92)	GSTIGSSP........TPPHHT.PP..PVssmagnTKNHPMLMNlL...KD...N..PAQD.FST......L..
  662-  719 (45.07/13.70)	....GSSPLerqnSSSGSPRME.MCSGSN......KTKKKKSSR.LppdKPKHQT...EDD.FQRelfsmdVdS
  848-  887 (51.26/16.72)	......SP......SSDSPTNHfFPDG.V......DFNPDLLNS.......QSQS.GFGEEyFDE......S.S
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     118.56|      37|      48|     146|     183|       6
---------------------------------------------------------------------------
  146-  183 (59.64/41.12)	NFDEFSKHLKGLVNlYNLP..GDNKLKTKMYLALQSLEQD
  196-  234 (58.93/36.34)	NAGPLDKILHGSVG.YLTPrsGGHLMNLKYYASPSDLLDD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.27|      14|      15|     991|    1005|       7
---------------------------------------------------------------------------
  991- 1005 (22.18/17.80)	KPGKRSRTPSnDGKS
 1008- 1021 (25.09/14.54)	KPTKRKKADT.EGKS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     148.32|      45|     163|     776|     823|      10
---------------------------------------------------------------------------
  776-  823 (70.21/40.65)	GPdvTDILSDIAEEASKLPsTSDDCPPIGTPVRDSSSSGHSQSALFDP
  942-  986 (78.10/36.94)	GP..ADLMEHHSGSQSPLL.TAGDLGKEKTQKRVKEGNGTSNNSLSGP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP27332 with Med1 domain of Kingdom Metazoa
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