<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP27314

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMQREEKQLEASVDALLSQVADLKSSLGSFICKLENEYDRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLLRNQVIIPLVLSPDRDEELTRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTADAARIGTDAAQKQIQTLNKMCSNLLEKISKEERESESGGLRQNKQTFNPTDTNALVAAVAFGKGLSNWRPPGSSGPGQPGQPGAGALLDSSGIQQVQMTGTPSQQQSTLLSGVQMAQTGQPGKMPSGIKTNIKSASMHPYQR
Length268
PositionHead
OrganismMonodelphis domestica (Gray short-tailed opossum)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Metatheria> Didelphimorphia> Didelphidae> Monodelphis.
Aromaticity0.03
Grand average of hydropathy-0.605
Instability index45.14
Isoelectric point6.14
Molecular weight29233.63
Publications
PubMed=17495919

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IBA:GO_Central
GO - Biological Function
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IBA:GO_Central
transcription coregulator activity	GO:0003712	IBA:GO_Central
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP27314
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     102.75|      32|      36|     177|     209|       1
---------------------------------------------------------------------------
  177-  209 (53.80/34.97)	DTNALVAAVAFGKGlSNWRPP..GSSGPGQPGQPG
  215-  248 (48.95/27.38)	DSSGIQQVQMTGTP.SQQQSTllSGVQMAQTGQPG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.45|      18|      34|      52|      69|       3
---------------------------------------------------------------------------
   52-   69 (30.04/23.04)	LSGQLNTLNKVLKHEKTP
   89-  106 (31.41/24.42)	LTRQTEGRVPVFSHEVVP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP27314 with Med8 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AVAFGKGLSNWRPPGSSGPGQPGQPGAGALLDSSGIQQVQMTGTPSQQQSTLLSGVQMAQTGQPGKMPSGIKTNIKSASMHPYQR
2) FSHEVVPDHLRTKPDPEVEEQEKQLTADAARIGTDAAQK
3) NLLEKISKEERESESGGLRQNKQTFNPTDTNALV
184
100
149
268
138
182










Molecular Recognition Features






MoRF SequenceStartStop




1) IKTNIKSASMHPYQR
254
268