<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26918

Description Mediator of RNA polymerase II transcription subunit 13
SequenceFPQADLTGIKWKRYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPNFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILSPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISSCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSSVGSSHCSASCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSAQKWIKFSSVSDGFSSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEETADKVASWDFVEATQRTNCSCLRHKNLKPRNPGQQGQAPSLSQQQQVLPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDTDSASQRLVISAPDSQVRFTNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLIPSKPVEDGIDSLSQSFPAQFQEAVEPTIYVGTAVNLEEDEANIAWKYYKVPKKKDLEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQYLSAIASDSEQEPKIDPYAFVEGDEEFLFPDKKDRQNNEREAGKKHKVEDGPSSVTVLSHEEDAMSLFSPSIKQDAPRPAGHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKSSCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSGIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQVLVGCSMFAPLKTLPSQCLPPIKLPEECIYRQSWTVGKLELLSSGPTMPFIKEGDGSTMDQEYGPAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTECGKEAEKRFEALRATSAEHVNGGLKESEKLPDELILLLQDQCTNLFSPFGAADQDPFPKIGVTNNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSVQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDFDFLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYECCRLGQHRPISRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNSEAFSKLKLYAQVCRYDLGPYLASQPLDSSLLSQPNLVAPTSQSLITPPQMTSTGNANTPSATLASTVSSTMTMSSGVAMSSSVATANSTLTTTSTSSSLSSNLNSGMSSNKLPSFPPFGTMNSSATGSMSTQASTVPNSQLGGQPPSALQTAGISGESSSVPTQPHPDVSESTMDRDKVGIPTDGDSHAITYPPAIVVYIIDPFTYENKDESTNSSNVWTLGLLRCFLEMVQTLPPHIKNTVSVQIVPCQYLLQPVKHEDRQIYTQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALKSPDRPECIRLYTPPFILAPVKDKQTELGETFGEPGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNSRARRKKGSARRFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSSTLNMQTSQLNTPQDISCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQGTDRLLSTESHDEVTNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPAIQDRRSCLPIHFVVLNQLYNFIMNML
Length2156
PositionMiddle
OrganismBos taurus (Bovine)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Artiodactyla> Ruminantia> Pecora> Bovidae> Bovinae> Bos.
Aromaticity0.08
Grand average of hydropathy-0.409
Instability index53.34
Isoelectric point5.40
Molecular weight237533.38
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364134

GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central
nucleoplasm	GO:0005654	IEA:Ensembl

GO - Biological Function
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
transcription coactivator activity	GO:0003713	IEA:Ensembl
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
cholesterol homeostasis	GO:0042632	IEA:Ensembl
negative regulation of DNA-binding transcription factor activity	GO:0043433	IEA:Ensembl
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
triglyceride homeostasis	GO:0070328	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP26918
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     226.36|      51|      68|     905|     969|       1
---------------------------------------------------------------------------
  905-  955 (91.68/31.88)	PSQCLPPIKLPEECIYR.QSWTVGKLELLS.SGPTMPFIKEG...DGSTMDQE.YGP
  974- 1025 (74.86/40.54)	PSNSGAGI.LPSPSTPRfPTPRTPRTPRTP.RGAGGPASAQG...SVKYENSDlYSP
 1569- 1618 (59.81/18.46)	.....PPSALQTAGISG.ESSSVPTQPHPDvSESTMDRDKVGiptDGDSHAIT.YPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      98.82|      20|     492|     532|     552|       2
---------------------------------------------------------------------------
  471-  488 (26.71/ 9.52)	TDSA..SQRLV.ISAPDSQVR
  500-  519 (37.11/16.16)	TPQM.HGTEMANSPQPPPLSP
  532-  552 (35.00/19.31)	TPSTpQSQHFYQMPTPDPLIP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     193.70|      47|      68|    1445|    1491|       3
---------------------------------------------------------------------------
 1445- 1491 (81.59/39.14)	LASQPLDSSLLSQPNLVAPTSQ.SLIT.PPQMTSTGNANTPSATLASTV
 1514- 1560 (71.85/33.57)	LTTTSTSSSLSSNLN.SGMSSN.KLPSfPPFGTMNSSATGSMSTQASTV
 1890- 1924 (40.27/15.54)	........SILS.ACLVAMEPQgSFVI.MPDSVSTGSVFGRSSTL....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     179.06|      54|      69|    1628|    1686|       4
---------------------------------------------------------------------------
 1628- 1686 (83.57/56.68)	FTYENKDESTnSSNVWTLGLLRCFLEMVQTL.PPHIKNTVSVQIVPcqYLLQPVKheDRQ
 1700- 1754 (95.49/48.98)	FTQCRRPLPT.STNVKTLTGFGPGLAMETALkSPDRPECIRLYTPP..FILAPVK..DKQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      90.89|      30|     202|     384|     421|       5
---------------------------------------------------------------------------
  387-  421 (43.78/42.71)	TADKVASWDFVEATQRTNcsclrHKNLKPRNPGQQ
  423-  452 (47.11/24.33)	QAPSLSQQQQVLPKHKTN.....EKQEKSEKPQKR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     214.99|      66|      69|     763|     831|       7
---------------------------------------------------------------------------
  762-  830 (106.76/52.50)	TDLDNLFNSDEDELTPGSKKSANGSDDKSSCK.ESKTGNLDPLSCiSTADLHKMYptPPSLEQHIMG...FSP
  831-  900 (108.23/45.91)	MNMNNKEYGSMDTTPGGTVLEGNSSGIGAQFKiEVDEGFCSPKPS.EIKDFSYVY..KPENCQVLVGcsmFAP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      96.99|      31|     713|     606|     642|       9
---------------------------------------------------------------------------
  606-  642 (47.52/36.66)	DLEFL...PPQLPS.DKFKDDPVGpfGQESVTSVtelmVQC
 1322- 1356 (49.47/23.76)	DFDFLvlsPFALPYwERLMLEPYG..SQRDIAYV....VLC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      69.45|      21|      37|     683|     705|      11
---------------------------------------------------------------------------
  683-  705 (33.99/26.06)	EGDEEFLF.PDKKdrQNNEREAGK
  722-  743 (35.46/20.30)	EEDAMSLFsPSIK..QDAPRPAGH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     121.05|      37|     817|    1245|    1282|      12
---------------------------------------------------------------------------
 1245- 1282 (59.58/47.22)	WSKRNDVSVQCSQdILRMLLSLQ.PVLQ.DAIQKKRTVRP
 2064- 2102 (61.47/43.36)	WSACPQAQYQCPL.FLKASLHLHvPSVQsDELLHSKHSHP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.77|      19|     972|     321|     352|      13
---------------------------------------------------------------------------
  321-  339 (33.51/28.92)	QSAQKWIKFSSVSDGFSSD
  367-  385 (33.27/ 7.94)	NRAQNKRKYSASSGGLCEE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP26918 with Med13 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) CSCLRHKNLKPRNPGQQGQAPSLSQQQQVLPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDTDSASQRLVISAPDSQVRFTNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLIPSKPVEDG
2) DLDNLFNSDEDELTPGSKKSANGSDDKSSCKESKT
3) FPDKKDRQNNEREAGKKHKVEDGPSSVTVLSHEE
4) GPTMPFIKEGDGSTMDQEYGPAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENS
5) SPVHSPGSHYPHGGDAGKGQGTDRLLSTESH
6) SSGVAMSSSVATANSTLTTTSTSSSLSSNLNSGMSSNKLPSFPPFGTMNSSATGSMSTQASTVPNSQLGGQPPSALQTAGISGESSSVPTQPHPDVSESTMDRDKVGIPTDGD
7) VAPTSQSLITPPQMTSTGNANTPSATLASTVSS
424
782
709
954
2022
1517
1480
578
816
742
1039
2052
1629
1512










Molecular Recognition Features






MoRF SequenceStartStop




1) AFVEGD
699
704