<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26653

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMQQREEKQLEASVESLISRVAHLKNSLQSFIYKLENEYDRMAWPSVLDNFALLSGQLNTINKLLRNEKTPSYRNQVIIPLLLSPDRDEELAKLTEQRVHVFSHEIVPDHLRTKPDPEVEEQEKQLSAEAARIGQEVAQKQIQTLNKLCSSLLEKLNNPRDDRDAETSAVRQNKQWFNPIDTTALVAAVGFGKGLSKCRPPASVAPGHPGQTMMTGGPTLQQVTIGPSGHQAGIGSSVAQQQPGQPGKMPSSIKTNIKSASMHPYNR
Length266
PositionHead
OrganismIctalurus punctatus (Channel catfish) (Silurus punctatus)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Actinopterygii> Neopterygii> Teleostei> Ostariophysi> Siluriformes> Ictaluridae> Ictalurus.
Aromaticity0.04
Grand average of hydropathy-0.634
Instability index44.46
Isoelectric point8.47
Molecular weight29515.09
Publications
PubMed=20634964
PubMed=23127152

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP26653
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.84|      28|      29|      77|     105|       1
---------------------------------------------------------------------------
   64-   92 (42.67/32.05)	LRNEKTPSYRNQVIiP..LLLSPDRD.EELAK
   93-  123 (39.17/24.53)	LTEQRVHVFSHEIV.PdhLRTKPDPEvEEQEK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      71.46|      20|      36|     189|     210|       2
---------------------------------------------------------------------------
  189-  210 (33.81/22.06)	GFGKGLSKcrPPASVAPGHPGQ
  228-  247 (37.65/18.27)	GHQAGIGS..SVAQQQPGQPGK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      95.03|      32|     118|       8|      39|       4
---------------------------------------------------------------------------
    8-   39 (52.80/38.83)	QLEASVESLISRVAHLK....NSL.QSFIYKLENEYD
  124-  160 (42.23/29.71)	QLSAEAARIGQEVAQKQiqtlNKLcSSLLEKLNNPRD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP26653 with Med8 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) SKCRPPASVAPGHPGQTMMTGGPTLQQVTIGPSGHQAGIGSSVAQQQPGQPGKMPSSIKTNIKSASMHPYNR
195
266










Molecular Recognition Features






MoRF SequenceStartStop




1) IKSASMHPYN
256
265