<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26574

Description Mediator of RNA polymerase II transcription subunit 5
SequenceMAVDRFLRQTRLSCSRGIQPTEWIENIKPNINTNALIDLILSTKPISVRLLTYYRIAIKTQEDHSETASHLIDLIPRIISQEEHDPQRILRILNPISLPLINYHNFTLNHHQAISQLQSLLQLIRLITPNQHHNQPTLSLAEHLLSALTNSILAHPANTRSIQQDILQSITTTIQDHHHHHPSQSTIYQYQLPSILRHLSPSTAKQLALNSSTTEQAPTATTTPSTTASSIIAYILLTPGVSALHSDDLEPQPILSRIISYALSSPSNFILQLFLAAISLGNSHPPSPLPNTRPKTEEIKSKEQDEDDDEDECLENDKMAWKALLAGGRLAELIRTALDTEILAKDREGIRLLGTDELVLMSQKILKKGAILDQSEPNEENRMEIENHRSYTNRHHLWFAILSSLCSNQLVLAQDLLRLDQSPSPHPNLVLGHHSDLDLGIHPLLDSLGKSSKETPISELAVKLHQSIVTATKSFDLSTCITLTEALSSLDSLSTLFLWIEPRKLLGPVRDLLDHWNDIRNNVNQLVNNSDNDESSSGSEFEKFGRLLGWLQGVVGRFSLMSNLSYHLGATTGFTIHHLSNPSTSYPISSLPASHQSTLSSWIEALYGSSGIPDDLLGHTDPRIFFSISASLFKQSFDALTIGLIDLQTFRDGLSYFEHKLLVGGCAVGVVGWLLNELTRVGRISASSYPTALLEILQSILLSDAITPTALHLVSARSLAVIRAFDASFSRAPPDHLGPGSDLNRRVQLDLGAIERRLTVLPASEMIEPAGFLAYDFGSATGWPDALDAAVRSAVRAEPDSPDPTIALWSLLPHILKAVSVGEFVQMTIRAAISASKMDDEHDSEEACTAQDRPGRIAVKVHKAWDAAKYRRVDRAVGVAVDILTWPVVGINHSGGRRPSLGPPGLVLQELFTHWGDRLGGSSSVEDAELERDIILACLDRLESLDLASTSLKKKSIPSESELPDDQSKLDPSSAMPQENRENGRGDNKLGDGERRTVTDRFRETCVTSLRRQKLRQFGLPEKREPRPVTPSRSDDLGKNAVDPFSPVSGTPMIVESGPDVPHREVGNQMDVQRSAVAEGEGAGVKELGDQEMGEKDGQEGDVKGDQQSGQKDGEQGEHNGSEKKEQAGQEKDEEGRQEKEGEQEKEKAEEGEKEDGGEKESLGEKKEAEEARGVTGAETETDDRPQEGEEGGKEAASGSADRMEVDRDADRGSEMDDRGAGSEVKKVHFLDRSAPPLARSMNGADQETSAEGDPEKKKKRKKTHAPSAALQIPSLPSTVLDWIIASLILRPVHNHLPLPLQLTPDDHFTTVLFSPDLLDHPALRFKLVGTIER
Length1334
PositionTail
OrganismPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Pucciniomycotina> Pucciniomycetes> Pucciniales> Pucciniaceae> Puccinia.
Aromaticity0.04
Grand average of hydropathy-0.443
Instability index53.12
Isoelectric point5.30
Molecular weight146658.83
Publications
PubMed=21536894

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats
>MDP26574
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     208.67|      36|      36|    1124|    1159|       1
---------------------------------------------------------------------------
 1067- 1097 (34.66/18.62)	...GNQMDVQRSA...VAEG..EGAGVKEL.GDQE...MGE......KD
 1098- 1125 (29.14/14.39)	...GQEGDVKGDQqsgQKDGEQ.......G.EHNG...SEK.......K
 1126- 1167 (54.59/33.91)	EQAGQEKDEEGRQ...EKEGEQEKEKAEEG.EKED...GGEkeslgeKK
 1168- 1189 (29.93/15.00)	E.A.....EEAR....GVTGA.......ET.ETDD...RPQ......EG
 1190- 1226 (35.25/19.08)	EEGGKEAASGSAD...RMEVDRD...ADRGsEMDDrgaGSE......VK
 1243- 1266 (25.10/11.29)	NGADQETSAEGDP...EKKKKRKKTHA......................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     183.07|      41|      42|     151|     192|       2
---------------------------------------------------------------------------
  118-  145 (32.61/13.47)	.............QS.LLQLIRLITPNQHHNQPT..LS.LAEHLL
  151-  192 (69.43/41.05)	SILAHPANTRSiQQD.ILQSITTTIQDHHHHHPS..QSTIYQYQL
  194-  236 (54.01/27.37)	SILRHLSPSTA.KQL.ALNSSTTEQAPTATTTPSttASSIIAYIL
  261-  287 (27.02/10.16)	YALSSPSN.......fILQLFLAAISLGNSHPPS...........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     167.15|      32|      49|     353|     388|       3
---------------------------------------------------------------------------
  353-  384 (51.81/25.66)	LGTDEL..VLMSQKILKKGAILDQSE.PNEENRME
  405-  430 (42.89/20.93)	LCSNQL..VL.AQDLLR....LDQS..PSPHPNLV
  901-  931 (47.16/22.26)	LGPPGL..VL.QELFTHWGDRLGGSS.SVEDAELE
  938-  970 (25.29/ 9.18)	.CLDRLesLDLASTSLKKKSIPSESElPDDQSKL.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     416.69|     113|     117|     603|     719|       4
---------------------------------------------------------------------------
  505-  584 (117.75/71.33)	...LLG.....PvRDLLD.HwND..IR...NNVNQLVNNS..........DNDESSSG.SEFEK...FG....RLLGW..LQGVVGRFS.LMSNLS.YH...............L.G.ATTGFTIHHLSNPST
  603-  719 (183.87/126.63)	IEALYGS.SGIP.DDLLG.H.TD..PRIFFSISASLFKQSFDALTIG.LIDLQTFRDGLSYFEHkllVGGCAvGVVGW..LLNELTRVG.RISASS.YPT....ALL.EILQSILLSDAITPTALHLVSARSL
  722-  835 (115.08/69.52)	IRAFDASfSRAP.PDHLGpG.SDlnRRVQLDLGA..IERRLTVLPASeMIEPAGF...LAY.DF.....GSA...TGWpdALDAAVRSAvRAEPDSpDPTialwSLLpHILKAVSVGEFVQMTIRAAISA...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.15|      18|      34|     978|    1010|       5
---------------------------------------------------------------------------
  978-  999 (26.20/45.03)	QENRENGRGDNKlgdgERRTVT
 1013- 1030 (32.96/12.89)	QKLRQFGLPEKR....EPRPVT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.68|      11|     129|     456|     467|       9
---------------------------------------------------------------------------
  456-  467 (16.15/14.58)	PISELAVKlHQS
  587-  597 (21.53/14.01)	PISSLPAS.HQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      76.90|      23|      49|      45|      71|      10
---------------------------------------------------------------------------
   45-   71 (34.47/36.01)	PISVRLLTYYRIAIktqeDHSETASHL
   95-  117 (42.42/31.85)	PISLPLINYHNFTL....NHHQAISQL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP26574 with Med5 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) HPPSPLPNTRPKTEEIKSKEQDEDDDEDEC
2) KKKSIPSESELPDDQSKLDPSSAMPQENRENGRGDNKLGDGERRTVTDRFRE
3) QKLRQFGLPEKREPRPVTPSRSDDLGKNAVDPFSPVSGTPMIVESGPDVPHREVGNQMDVQRSAVAEGEGAGVKELGDQEMGEKDGQEGDVKGDQQSGQKDGEQGEHNGSEKKEQAGQEKDEEGRQEKEGEQEKEKAEEGEKEDGGEKESLGEKKEAEEARGVTGAETETDDRPQEGEEGGKEAASGSADRMEVDRDADRGSEMDDRGAGSEVKKVHFLDRSAPPLARSMNGADQETSAEGDPEKKKKRKKTHAPSAALQ
284
953
1013
313
1004
1272










Molecular Recognition Features






MoRF SequenceStartStop




1) DPEKKKKRKKTHA
2) EQAGQE
3) VKKVHFLDR
1254
1126
1225
1266
1131
1233