<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26460

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSLVTSANSVDLPACFFLKFPQPLPVSRAFVQKIQNCTGIPLFETQPTYVPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQANNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPTDLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGASNSSLSGTGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSLSSASNSGKMKSSKSEGSSSSKLSSSIYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSHPVSSKHNMSGGEFQGKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGEGGGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN
Length1575
PositionMiddle
OrganismBos taurus (Bovine)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Artiodactyla> Ruminantia> Pecora> Bovidae> Bovinae> Bos.
Aromaticity0.04
Grand average of hydropathy-0.671
Instability index52.86
Isoelectric point8.88
Molecular weight167792.63
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


GO - Cellular Component
mediator complex	GO:0016592	ISS:UniProtKB
nucleolus	GO:0005730	ISS:UniProtKB
nucleoplasm	GO:0005654	IEA:Ensembl
nucleus	GO:0005634	ISS:UniProtKB

GO - Biological Function
chromatin binding	GO:0003682	ISS:UniProtKB
DNA binding	GO:0003677	IEA:Ensembl
estrogen receptor binding	GO:0030331	IEA:Ensembl
LBD domain binding	GO:0050693	IEA:Ensembl
nuclear receptor coactivator activity	GO:0030374	ISS:UniProtKB
peroxisome proliferator activated receptor binding	GO:0042975	IEA:Ensembl
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IBA:GO_Central
thyroid hormone receptor binding	GO:0046966	ISS:UniProtKB
transcription coactivator activity	GO:0003713	ISS:UniProtKB
transcription coregulator activity	GO:0003712	ISS:UniProtKB
transcription corepressor activity	GO:0003714	ISS:UniProtKB
vitamin D receptor binding	GO:0042809	IBA:GO_Central

GO - Biological Process
androgen biosynthetic process	GO:0006702	ISS:UniProtKB
angiogenesis	GO:0001525	ISS:UniProtKB
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	ISS:UniProtKB
cellular response to epidermal growth factor stimulus	GO:0071364	ISS:UniProtKB
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to thyroid hormone stimulus	GO:0097067	IBA:GO_Central
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
erythrocyte development	GO:0048821	ISS:UniProtKB
fat cell differentiation	GO:0045444	IEA:Ensembl
intracellular steroid hormone receptor signaling pathway	GO:0030518	ISS:UniProtKB
keratinocyte differentiation	GO:0030216	ISS:UniProtKB
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	ISS:UniProtKB
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	ISS:UniProtKB
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	ISS:UniProtKB
negative regulation of apoptotic process	GO:0043066	ISS:UniProtKB
negative regulation of keratinocyte proliferation	GO:0010839	ISS:UniProtKB
negative regulation of neuron differentiation	GO:0045665	ISS:UniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	ISS:UniProtKB
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IEA:Ensembl
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	ISS:UniProtKB
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	ISS:UniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	ISS:UniProtKB
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	ISS:UniProtKB
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of RNA biosynthetic process	GO:2001141	ISS:UniProtKB
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	ISS:UniProtKB
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP26460
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
            11|     538.73|      52|      52|     997|    1048|       1
---------------------------------------------------------------------------
  641-  693 (43.94/ 7.97)	NHP.MLMN....................L..LKDNP......AQ......DF.....................STLYGSSPLERQNS..SSGSPrmEM...CSGSNKTKK.K..KSS
  747-  816 (40.53/ 6.82)	TPP.TTYP....................Q..PVPHP.....qPS.....IQRmvrlsssdsigpdvtdilsdiAEEASKLPSTSDD.....CPP..IG...T.PVRDSSS.S..GHS
  997- 1048 (99.29/26.75)	RTP.SNDG....................K..SKDKP......PK.....RKK.....................ADTEGKSPSHSSNR..PFTPP..TS...TGGSKSPGS.S..GRS
 1055- 1098 (39.65/ 6.52)	ATP.P..............................I......PKitiqiPKG.....................TVMVGKPSSHSQ.....Y.....TS...SGSVSSSGSkS..HHS
 1103- 1138 (47.44/ 9.16)	SSA.SNSG...................................K.....MKS.....................SKSEGSSSSKLSSS..IYS..............SQGS.S..GSS
 1141- 1193 (42.02/ 7.32)	KNS.SQSG.........................GKP...gssPI.....TKH.........................GLSSGSSSTKmkPQGKP..SSlmnPSLSKPNIS.P..SHS
 1194- 1227 (41.87/ 7.27)	RPP.GGSD....................KlaSPMKP......V..............................................PGTPP..SS...K..AKSPIS.S..GSG
 1233- 1271 (47.63/ 9.23)	GTS.SSTG.........................................MKS.....................SSGLGSSGSLS..Q..K.TPP..SS.....NSCTASS.S..SFS
 1339- 1384 (42.54/ 7.50)	KHN.MSGG....................E..FQG.........K.....REK.....................SDKD.KSKVSTSGG..SVDSS..KK...TSESKNVGS.T..G..
 1420- 1471 (48.21/ 9.42)	QMA.S..S....................K..NYGSPlisgstPK.....HER.........................G.SPSHSKSP..AYTPQ..NL...DSESES.GS.SiaEKS
 1474- 1537 (45.62/ 8.55)	NSPsSDDGirplpeystekhkkhkkekkK..VKDKD......RD.....RDK......................DRD.KKKSHSI.K..P......ES.....WSKSPIS.S..DQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     201.59|      49|     340|     556|     615|       2
---------------------------------------------------------------------------
   71-  105 (33.45/ 6.03)	.................VTSLPAMT....DRLESIarqnGLGSH.LSAS..GTECYITS
  556-  606 (83.76/50.80)	PMSgTTTPTN.TFPGGPITTLFNMSMsIKDRHESV....GHGED.FSKV..SQNPILTS
  696-  725 (29.47/ 6.04)	............PPDKP...........KHQTED.....DFQRElFSMDvdSQNPIFD.
  848-  888 (54.92/19.50)	SPS.SDSPTNhFFPDG.VD..FNPDL.LNSQSQS.....GFGEEyFDES..SQ......
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     118.56|      37|      48|     146|     183|       3
---------------------------------------------------------------------------
  146-  183 (59.64/38.47)	NFDEFSKHLKGLVNlYNLP..GDNKLKTKMYLALQSLEQD
  196-  234 (58.93/34.02)	NAGPLDKILHGSVG.YLTPrsGGHLMNLKYYASPSDLLDD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     100.07|      15|      45|     298|     312|       4
---------------------------------------------------------------------------
  298-  312 (30.80/19.24)	DLPACFFL.KFPQPLP
  326-  338 (20.95/10.40)	GIP..LFE.TQPTYVP
  341-  356 (20.49/ 9.98)	ELITQFELsKDPDPLP
  369-  383 (27.83/16.57)	GQQHCYFL.NKDAPLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.01|      17|      23|     938|     960|       6
---------------------------------------------------------------------------
  949-  965 (31.23/14.53)	HHSGSQS.........PLLTTGD.LGK
 1393- 1419 (21.78/ 6.85)	HDGGSPSikakvtlqkPGEGGGEgLRP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      68.91|      20|     340|     108|     144|       7
---------------------------------------------------------------------------
  115-  139 (33.67/48.69)	DPagqlcDVKVAHHGENPVSCP.ELV
  822-  842 (35.25/ 9.88)	DP.....DVFQANNNENPYTDPaDLI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     177.37|      43|      53|     396|     438|       8
---------------------------------------------------------------------------
  396-  438 (73.10/47.88)	ITF.....QHPGRVPLILNLIRHQVAYNTLIG.............SCVKRTILKEDSPGLL
  452-  488 (60.28/38.06)	VSF.....QHPVNDSLV..CVVMDVQDSTHV..............SC...KLYKGLSDALI
  491-  547 (43.98/25.56)	DDFiakvvQRCMSIPVTMRAIRRKA..ETIQAdtpalsliaetveDMVKKNLPPASSPG..
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP26460 with Med1 domain of Kingdom Metazoa
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