<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26126

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMSSLLDRLHAKFNQSRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQADTVDFSILSVAGKALGAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGDSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDDGVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1567
PositionMiddle
OrganismRattus norvegicus (Rat)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Glires> Rodentia> Myomorpha> Muroidea> Muridae> Murinae> Rattus.
Aromaticity0.04
Grand average of hydropathy-0.672
Instability index53.43
Isoelectric point8.88
Molecular weight166408.81
Publications
PubMed=15057822
PubMed=15632090
PubMed=22673903

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364059
GO - Cellular Component
chromatin	GO:0000785	IDA:RGD
mediator complex	GO:0016592	ISS:UniProtKB
nucleolus	GO:0005730	ISS:UniProtKB
nucleoplasm	GO:0005654	IEA:Ensembl
nucleus	GO:0005634	IDA:RGD
protein-DNA complex	GO:0032993	IDA:RGD
ubiquitin ligase complex	GO:0000151	IDA:RGD
GO - Biological Function
chromatin binding	GO:0003682	IDA:RGD
chromatin DNA binding	GO:0031490	IDA:RGD
DNA binding	GO:0003677	ISO:RGD
estrogen receptor binding	GO:0030331	ISO:RGD
LBD domain binding	GO:0050693	ISO:RGD
nuclear hormone receptor binding	GO:0035257	ISO:RGD
nuclear receptor binding	GO:0016922	ISO:RGD
nuclear receptor coactivator activity	GO:0030374	ISS:UniProtKB
peroxisome proliferator activated receptor binding	GO:0042975	ISO:RGD
promoter-specific chromatin binding	GO:1990841	ISO:RGD
protein-containing complex binding	GO:0044877	IDA:RGD
retinoic acid receptor binding	GO:0042974	ISO:RGD
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IDA:RGD
thyroid hormone receptor binding	GO:0046966	IDA:RGD
transcription coactivator activity	GO:0003713	ISS:UniProtKB
transcription coregulator activity	GO:0003712	IDA:RGD
transcription corepressor activity	GO:0003714	ISS:UniProtKB
transcription factor binding	GO:0008134	ISO:RGD
vitamin D receptor binding	GO:0042809	IDA:RGD
GO - Biological Process
androgen biosynthetic process	GO:0006702	ISS:UniProtKB
angiogenesis	GO:0001525	ISS:UniProtKB
animal organ morphogenesis	GO:0009887	ISO:RGD
animal organ regeneration	GO:0031100	ISO:RGD
brain development	GO:0007420	IERGD
camera-type eye development	GO:0043010	ISO:RGD
cell morphogenesis	GO:0000902	ISS:UniProtKB
cellular response to epidermal growth factor stimulus	GO:0071364	ISS:UniProtKB
cellular response to hepatocyte growth factor stimulus	GO:0035729	ISO:RGD
cellular response to steroid hormone stimulus	GO:0071383	ISO:RGD
cellular response to thyroid hormone stimulus	GO:0097067	IDA:RGD
embryonic heart tube development	GO:0035050	ISO:RGD
embryonic hemopoiesis	GO:0035162	ISO:RGD
embryonic hindlimb morphogenesis	GO:0035116	ISO:RGD
embryonic placenta development	GO:0001892	ISO:RGD
enucleate erythrocyte development	GO:0048822	ISO:RGD
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	ISO:RGD
erythrocyte development	GO:0048821	ISS:UniProtKB
fat cell differentiation	GO:0045444	ISO:RGD
heart development	GO:0007507	ISO:RGD
in utero embryonic development	GO:0001701	ISO:RGD
intracellular steroid hormone receptor signaling pathway	GO:0030518	ISS:UniProtKB
keratinocyte differentiation	GO:0030216	ISS:UniProtKB
lactation	GO:0007595	ISO:RGD
lens development in camera-type eye	GO:0002088	ISS:UniProtKB
liver development	GO:0001889	ISO:RGD
mammary gland branching involved in pregnancy	GO:0060745	ISO:RGD
mammary gland branching involved in thelarche	GO:0060744	ISO:RGD
mammary gland epithelial cell proliferation	GO:0033598	ISO:RGD
megakaryocyte development	GO:0035855	ISS:UniProtKB
monocyte differentiation	GO:0030224	ISO:RGD
mRNA transcription by RNA polymerase II	GO:0042789	ISS:UniProtKB
multicellular organism development	GO:0007275	ISO:RGD
negative regulation of apoptotic process	GO:0043066	ISS:UniProtKB
negative regulation of keratinocyte proliferation	GO:0010839	ISS:UniProtKB
negative regulation of neuron differentiation	GO:0045665	ISS:UniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	ISS:UniProtKB
peroxisome proliferator activated receptor signaling pathway	GO:0035357	ISO:RGD
positive regulation of cell population proliferation	GO:0008284	ISO:RGD
positive regulation of erythrocyte differentiation	GO:0045648	ISO:RGD
positive regulation of G0 to G1 transition	GO:0070318	ISO:RGD
positive regulation of gene expression	GO:0010628	ISS:UniProtKB
positive regulation of hepatocyte proliferation	GO:2000347	ISO:RGD
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	ISO:RGD
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	ISO:RGD
positive regulation of keratinocyte differentiation	GO:0045618	ISS:UniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	IDA:RGD
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	ISS:UniProtKB
positive regulation of transcription, DNA-templated	GO:0045893	ISO:RGD
protein import into nucleus	GO:0006606	ISO:RGD
protein ubiquitination	GO:0016567	IDA:RGD
regulation of RNA biosynthetic process	GO:2001141	ISO:RGD
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of transcription, DNA-templated	GO:0006355	ISO:RGD
regulation of vitamin D receptor signaling pathway	GO:0070562	ISO:RGD
retinal pigment epithelium development	GO:0003406	ISO:RGD
thyroid hormone generation	GO:0006590	ISO:RGD
thyroid hormone mediated signaling pathway	GO:0002154	ISS:UniProtKB
ventricular trabecula myocardium morphogenesis	GO:0003222	ISO:RGD

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP26126
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
            10|     959.79|     100|     100|    1034|    1133|       1
---------------------------------------------------------------------------
  520-  597 (53.32/ 8.20)	............................................MVK.KnlpPASSP............GY.......G.mTTGSNPMSGTTTPTNtfPGG.PITTLFN.MSMSIKDRhESVGHGEDF..SK........V.........SQNPILTSLLQ..IT....G
  607-  712 (87.93/18.60)	P...TP....P....HHTPPPV.................SS...MAG.N...TKNHPmlmnllkdnpaqDF.......S.....TLYGSSPLERQN..SSS.GSPRMEM.CSGSNKTK.KNKSSRVPP..DKpkhqteddFQ..R.ELFSMD.....VDSQ..NPmfdvS
  713-  809 (101.80/22.77)	M...TA....D....ALDTPHI.TPAPS.......QCSTPPT..TYP.Q...PVSHP............QP.......SiqRMGRLSSSDSIG......PD.VTDILSDiAEEASKLP.ST....SDD..CP........PI..G.TPVRDSSSSGHSQSAlfDS....D
  812-  909 (91.39/19.64)	Q...TN....N....N..ENPY.TD............PADLIadAAG.S...PSSDS.........ptnHF.......F..PDGVDFNPDLLNSQS..QSGfGEEYFDE.SSQSGDND.DFK..GFAS..QA........LN..TlGMPMLGGDNGESKLK..GS....S
  910- 1030 (95.65/20.92)	Q.adTV....DfsilSVAGKAL.GAADLlehhsgsQSPLLTT.gELG.K...EKTQK............RVkegngmgA..SSGSGPGSDSKPGKR..SRT.PSNDGKS.KDKPPKRK.KADTEGKSP..SH........SS..N.RPFTPPTSTGGSKSP..GS....S
 1034- 1133 (168.16/42.71)	Q...TP....P....GVATPPI.PKITI.......QIPKGTV..MVG.K...PSSHS............QY.......S..SSGSVSSSGSKSHHS..HSS.SSSSLSS.ASTSGKMK.SSKSEGSSS..AK........LS..G.SMYSSQGSSGSSQSK..NS....S
 1134- 1188 (72.13/13.85)	Q...TG..gkP....G..SSP...............ITK................H...........................G..............LSS.GSSS........TKM....KPQGKPS..SL........MN..P.SISKPNISPSHSRPP..GG....S
 1192- 1282 (111.50/25.69)	A...SPmkpvP....G..TPPS.SK............AKSPI..SSG.S...GSSH.........................VSGTSSSSGMKS.SS..GSG.SSGSLSQ.KTPPAS...NSCTPSSSS..FS........SS..GsSMSSSQNQHGSSKGK..SP....S
 1285- 1371 (92.67/20.03)	K...KP....S......LTAVI.DKL...........KHGVV..TSG.....P..............................GGEDPIDSQMGAS..TNS.SNHPMSSkHNMSGGEF.QSKREKSDKdkSK........VS..T.SGGSVDSSKKTSESK..NV....G
 1377- 1471 (85.23/17.79)	KiiiSK....H....DGGSPSIkAKVTL.......QKPGDSG..GEGlR...PQIAS............S........K..NYGSPLISGSTPKHE..RGS.PSHSKSP.AYTPQNVD.SESESGSSI..AE........RSyqN.SPSSDDG.................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     136.12|      44|     340|      86|     135|       2
---------------------------------------------------------------------------
   86-  135 (67.78/59.67)	CYITSDMFYVEVQlDPAGQ..LC...DVKVAHHgenpVSCpELVQQLREK..NFDEF
  428-  478 (68.34/40.90)	CPLSESRFSVSFQ.HPVNDslVCvvmDVQDSTH....VSC.KLYKGLSDAliCTDDF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.55|      15|      41|     283|     297|       4
---------------------------------------------------------------------------
  283-  297 (31.39/19.22)	DLPACFFL.KFPQPIP
  326-  341 (24.16/12.92)	ELITQFELsKDPDPLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     103.03|      31|      48|     137|     168|       6
---------------------------------------------------------------------------
  137-  168 (49.78/27.55)	KHLKGLVNlYNLP..GDNKLKTKMYLALQSLEQD
  187-  219 (53.25/26.06)	KILHGSVG.YLTPrsGGHLMNMKYYASPSDLLHD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.52|      12|      15|    1481|    1492|       7
---------------------------------------------------------------------------
 1481- 1492 (19.60/13.52)	EKHKKHKKEKKK
 1499- 1510 (19.91/13.86)	DRDRDKDRDKKK
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP26126 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFN
2) GAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGDSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDDGVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN
3) KNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGP
4) QSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQADTV
561
927
523
858
851
1567
556
914










Molecular Recognition Features






MoRF SequenceStartStop




1) FGEEYFDE
2) IPKITIQIPK
3) KPSLTAVIDKLKHGV
4) LPEYSTEKHKKHKKEKKKVKDKDRDR
5) LRPQIA
6) LSPDFMIGEEDDDLMDV
7) SIKAKVTLQ
8) VAKIIISKH
863
1044
1286
1475
1407
1546
1389
1375
870
1053
1300
1500
1412
1562
1397
1383