<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26070

Description Isoform 2 of Cyclin-dependent kinase 8
SequenceMDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY
Length463
PositionKinase
OrganismHomo sapiens (Human)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Hominidae> Homo.
Aromaticity0.09
Grand average of hydropathy-0.706
Instability index39.97
Isoelectric point8.72
Molecular weight53283.07
Publications
PubMed=7568034
PubMed=15057823
PubMed=15489334
PubMed=9734358
PubMed=10024883
PubMed=10993082
PubMed=15546612
PubMed=15175163
PubMed=15989967
PubMed=17000779
PubMed=19369195
PubMed=30905399
PubMed=21806996
PubMed=17344846

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C- terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. 
ECO:0000269	PubMed:10993082
ECO:0000269	PubMed:15546612
ECO:0000269	PubMed:30905399
GO - Cellular Component
mediator complex	GO:0016592	IDA:MGI
nucleolus	GO:0005730	IDA:MGI
nucleoplasm	GO:0005654	TAS:Reactome
nucleus	GO:0005634	IDA:MGI
protein-containing complex	GO:0032991	IDA:MGI
GO - Biological Function
ATP binding	GO:0005524	IEA:UniProtKB-KW
cyclin-dependent protein serine/threonine kinase activity	GO:0004693	IBA:GO_Central
protein kinase activity	GO:0004672	IMP:UniProtKB
protein serine kinase activity	GO:0106310	IEA:RHEA
protein threonine kinase activity	GO:0106311	IEA:RHEA
RNA polymerase II CTD heptapeptide repeat kinase activity	GO:0008353	IBA:GO_Central
GO - Biological Process
F:protein kinase activity	GO:0004672	IMUniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	TAS:Reactome
protein phosphorylation	GO:0006468	IBA:GO_Central
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	TAS:Reactome

Interaction

Binary Interactions
[P49336<-->P24863: CCNC]	NbExp=36	EBI-394377,EBI-395261
[P49336<-->Q01094: E2F1]	NbExp=3	EBI-394377,EBI-448924
[P49336-2<-->A0A140G945: CRYAA2]	NbExp=3	EBI-11039720,EBI-25838900
[P49336-2<-->Q14204: DYNC1H1]	NbExp=3	EBI-11039720,EBI-356015
[P49336-2<-->Q92876: KLK6]	NbExp=3	EBI-11039720,EBI-2432309

Repeat regions

Repeats

>MDP26070
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     176.32|      54|      87|     209|     265|       2
---------------------------------------------------------------------------
  209-  265 (87.19/65.29)	RHYTKAiDIWAIGcIFAELLTSEPIFHCRQEDiKTSNPYH.HDQL..DRIFNVMGFPADK
  299-  355 (89.13/53.39)	KHKVKP.DSKAFH.LLQKLLTMDPIKRITSEQ.AMQDPYFlEDPLptSDVFAGCQIPYPK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.44|      15|      36|     151|     165|       4
---------------------------------------------------------------------------
  151-  165 (26.77/17.68)	DLKPANILVMGEGPE
  189-  203 (28.67/19.41)	DLDPVVVTFWYRAPE
---------------------------------------------------------------------------

Associated diseases

Disease
Cardiovascular diseases , cancer	PMID:24751643 PMID:24746821 PMID:33257171 PMID:24754906 PMID:29266937 PMID:32463833 PMID:32752128 PMID:28220308 PMID:29780169
breast cancer	PMID:31988137 PMID:24427329 PMID:32078847 PMID:26452386 PMID:31622095 PMID:28147342 PMID:31087707 PMID:33173974
colon cancer	PMID:31988137 PMID:28231524 PMID:21806996 PMID:20514474 PMID:19808961 PMID:26235356 PMID:22104393 PMID:33291686 PMID:29967145
prostate cancer	PMID:27678455 PMID:32078847
melanoma	PMID:22035865 PMID:32078847 PMID:23454913 PMID:26235356
colorectal cancers	PMID:22035865 PMID:30594029 PMID:22945643 PMID:28191946 PMID:27490956 PMID:23454913 PMID:18794900 PMID:21179167 PMID:19790197 PMID:32596239
endometrial cancer	PMID:31520353 PMID:23454913
uterine cancer	PMID:31520353
gastric cancer	PMID:28231524
lung cancer	PMID:32802198
acute myeloid leukemia	PMID:31382571
cancer  cardiovascular diseases  metabolic diseases	PMID:30352217
inflammatory disorders  viral infections  cancer	PMID:28855340
Gastric adenocarcinoma	PMID:21344156
pancreatic cancer	PMID:29856990 PMID:25305448
colon cancer hepatic metastasis	PMID:30185549
cardiovascular diseases	PMID:31255603 PMID:30352217
Congenital heart disease	PMID:30905399 PMID:24751643
inflammation diseases	PMID:33257171
metabolic diseases	PMID:30352217
periodontitis	PMID:32978798


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP26070 with CDK8 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) IPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY
351
464










Molecular Recognition Features






MoRF SequenceStartStop




NANANA