<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP26069

Description Cyclin-dependent kinase 8
SequenceMDYDFKMKTQIERTKVEDLFNYEGCKVGRGTYGHVYKAKWKETSDGKEYALKQIDGTGLSMSACREIALLRELKHQNVITLIRVFLSHNDRKVFLLIDYAEHDLWHIIKFHRAAKATKKQVVVPRGMVKSLLYQILDGIHYLHSNWVLHRDLKPANILVMGDGNERGRVKIADMGFARLFNAPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPQDKDWEDIKKMPEHHTLTKDFKRSTYSTCSLAKYMERHKIKPDSKAFHLLQKLLLMDPNKRITSEQAMQDQYFQEEPQPTQDVFAGCPIPYPKREFLTDDDQEDKSDNKRQQQQQQQQQQQQQQQQQQQQQQQQQQQQMNAEPNAKRVRLSGAGNQQDFHHQQQQQQQQQQQQQQQQQQMMFNQQQNFQRFN
Length454
PositionKinase
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.09
Grand average of hydropathy-0.908
Instability index56.48
Isoelectric point8.77
Molecular weight53681.24
Publications
PubMed=8730095
PubMed=10731132
PubMed=12537572
PubMed=12537569
PubMed=17290221

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C- terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation. 
ECO:0000269	PubMed:17290221
ECO:0000269	PubMed:8730095
GO - Cellular Component
mediator complex	GO:0016592	IDA:FlyBase
nucleus	GO:0005634	IDA:UniProtKB
GO - Biological Function
ATP binding	GO:0005524	IEA:UniProtKB-KW
cyclin-dependent protein serine/threonine kinase activity	GO:0004693	IBA:GO_Central
kinase activity	GO:0016301	IDA:FlyBase
protein serine kinase activity	GO:0106310	IEA:RHEA
protein threonine kinase activity	GO:0106311	IEA:RHEA
RNA polymerase II CTD heptapeptide repeat kinase activity	GO:0008353	IBA:GO_Central
GO - Biological Process
chaeta development	GO:0022416	IMFlyBase
G1/S transition of mitotic cell cycle	GO:0000082	IMUniProtKB
imaginal disc-derived leg segmentation	GO:0036011	IMFlyBase
positive regulation of autophagy	GO:0010508	IMFlyBase
protein phosphorylation	GO:0006468	IDA:UniProtKB
sex comb development	GO:0045498	IMFlyBase
snRNA 3'-end processing	GO:0034472	IDA:FlyBase

Interaction

Binary Interactions
[Q9VT57<-->P25008: CycC]	NbExp=2	EBI-163640,EBI-195485
[Q9VT57<-->Q27368: E2f1]	NbExp=2	EBI-163640,EBI-108384

Repeat regions

Repeats

>MDP26069
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      98.40|      15|      15|     370|     384|       1
---------------------------------------------------------------------------
  370-  384 (32.98/11.57)	NKRQQQQQQQQQQQQ
  386-  400 (32.71/11.42)	QQQQQQQQQQQQQQQ
  427-  441 (32.71/11.42)	QQQQQQQQQQQQQQQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.71|      16|      27|     239|     256|       2
---------------------------------------------------------------------------
  239-  256 (23.28/20.70)	EDIKtSNPYHHdQLDRIF
  268-  283 (31.43/17.43)	EDIK.KMPEHH.TLTKDF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.02|      19|      35|     135|     154|       3
---------------------------------------------------------------------------
  135-  154 (34.79/20.06)	ILD.GIHYLHsNWVLH..RDLKP
  171-  192 (22.22/ 8.76)	IADmGFARLF.NAPLKplADLDP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      83.20|      25|      40|     284|     309|       6
---------------------------------------------------------------------------
  284-  309 (39.15/29.10)	KRSTYSTCSLAKYMERHKiKPDSKAF
  322-  346 (44.04/28.18)	KRITSEQAMQDQYFQEEP.QPTQDVF
---------------------------------------------------------------------------


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP26069 with CDK8 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) PNKRITSEQAMQDQYFQEEPQPTQDVFAGCPIPYPKREFLTDDDQEDKSDNKRQQQQQQQQQQQQQQQQQQQQQQQQQQQQMNAEPNAKRVRLSGAGNQQDFHHQQQQQQQQQQQQQQQQQQMMFNQQQNFQRFN
320
454










Molecular Recognition Features






MoRF SequenceStartStop




1) AEPNAKRVRLS
403
413