<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP25986

Description RNA polymerase II mediator complex component Srb8
SequenceMNASSTEQTARPPWSFRSDIRRTGPGAQTELAQWSQSASLPPLPIRPWKYGIQGRHVAGATNQKDNEECGEVTTTPYRIDVPSIAPRFADNKPADFTPWRGSHPEDVLSEQTIKQGFYDRSPAPPTESTSARTSVYPHFKRRSGLKLLSSVFAAALEKRQALCTITAASTFRPPPRVTLTDIKREAWLRDLANPSVPLRRLSRTIPHGIRGKLLLDQCLGKSIPICRAIWLAKCVGANELRAFKRKGTSAGVASGLETKWVRDWTISVQQFIEGVIASFGDASWKLRISYSIRLSARLFLEHLIEQDHFLDWFLTSLDNSSFESLPVWLLMVGVYWQNIVRYRKRAKRLAECLLEKLRLVFDAGQNAHLNPLAQRLSRLTKTLTLYHPSSFILPRTWAKYESVLSSCLDMNLPDDKAAFQNLKARNTRVCRLQNDRGKPIQSINQRLIQLLDSSSDVSTICSECLNISQDYSILVTKLIEWASTSFRYGTARTYTVVRVFRKWKRSGVDIDSHILSFLIQDHRKSGVRFFDVYHVICELVRSQSFSVAKYLQWLMARGVLSNVSDFQQMHVSADIGLLGHLPPHRLPSHIWNLRNTLLSRTGFSVALENATIQLIKASVRRRLPHMFPMLSIDGDAEMTDDVDLTSLSWTVRSEIGNWIRDQVASHVKNSLKSAIDRDFASEVKTSALTPQEFFEVRYILECLCDFSMLADVLKYASGSNNVTVVASAVDTLNYHLDSFNAIGATGDLFKSFTGAYARISKTELSVQDLIASLLDVAIKLPAELSTLSMLRRDQLQRDRKLAMAACSPVSDHIVDTLNTANPTFTEELDQLLTSGNSMDESTLARIFDTLRKKLEAGSVNGEQSAHETARYFANLRPFNTKLFDNLMIKWVISVMRSSPRPKLSSILPPLIGVGCVTLHSFYVLARAFLHSDAHKNAIPDLAELRIDMIQLLDDKLSNGNGSQDLVAYRFKIARQGYMRQYSGEALGLIQDLLADLSENGADTPPKQFKNDLLSTIVTPLFCEVIVRHPSIVGADCRDRLLETFTACMNLTHQALGRLLASPVQTGLAAAERTIESINDFSLPLCLINLRLLFSFECDNDVKKRIYDVVFEAAKSNMHKGQSHWIDVVGTLHADAAKEVSRIPSELQFDGSADILFSFFFFAKIRQRAEEQLLSLVLSSDSLPASPVSKSGAPTSVTASALVCLRIVEDLSFSVPENGSPSLGPMLIEKMNMVLHRITILESHIMNTNIMQYNDQAAAIRQAHATQESAITFWFYILLRLVSIHRSTFTPGTLSKSDLADQSRLLISITCIALSKTLSPKVPSARPYYIPPTTPLMFAVPPQGSQPQPLFYSTDAGISTNLRTQALDVAATLLDSIPDDSRHQCSRFFRDRCPPFLHPQNNPRLFFLLGPLAADTQPSSSTMQSSQQQPVAPSLSQTTLGPPASTATPAAGAPPGAKSNSSSSQQFQSGNIYNTTNFLPFDDQNSLVGKLRVQHPGGIVGRFPLRPWEMLEESAPVIGVNDTAVDLGWFAARRVRGDVI
Length1539
PositionKinase
OrganismAjellomyces capsulatus (strain H143) (Darling's disease fungus) (Histoplasma capsulatum)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Eurotiomycetidae> Onygenales> Ajellomycetaceae> Histoplasma.
Aromaticity0.08
Grand average of hydropathy-0.143
Instability index46.51
Isoelectric point8.93
Molecular weight171373.10
Publications









Function






Annotated function

Component of the SRB8-11 complex. The SRB8-11 complex is a
regulatory module of the Mediator complex which is itself involved in
regulation of basal and activated RNA polymerase II-dependent
transcription. The SRB8-11 complex may be involved in the
transcriptional repression of a subset of genes regulated by Mediator.
It may inhibit the association of the Mediator complex with RNA
polymerase II to form the holoenzyme complex.


Component of the srb8-11 complex. The srb8-11 complex is a
regulatory module of the Mediator complex which is itself involved in
regulation of basal and activated RNA polymerase II-dependent
transcription. The srb8-11 complex may be involved in the
transcriptional repression of a subset of genes regulated by Mediator.
It may inhibit the association of the Mediator complex with RNA
polymerase II to form the holoenzyme complex.



GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP25986
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     132.48|      41|      83|     703|     748|       1
---------------------------------------------------------------------------
  703-  739 (57.09/33.00)	.......................................LCDFSML.ADV..L....KYASGSNNVTVVASAVDTLNYHLD..SF
  745-  824 (39.12/27.96)	TGDLfksftgayarisktelsvqdliaslldvaiklpaeLSTLSMLrRDQ..LqrdrKLAMAACS.PVSDHIVDTLNTANP..TF
  825-  859 (36.26/14.72)	TEEL..............................................dqL....LTSGNSMDESTLARIFDTLRKKLEagSV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      83.25|      28|     607|     752|     783|       2
---------------------------------------------------------------------------
  752-  783 (38.31/38.62)	FTGAYARISkTELSVQ..DLIASLLDvaiKLPAE
 1350- 1379 (44.94/30.67)	FYSTDAGIS.TNLRTQalDVAATLLD...SIPDD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     166.34|      53|     607|     861|     913|       3
---------------------------------------------------------------------------
  861-  913 (93.70/54.45)	GEQSAHETARYF........ANLRPFNTK..LFDNLMIKWVISVMRSSP.RP.KLSSILPPLIGV
 1455- 1519 (72.64/40.58)	GAKSNSSSSQQFqsgniyntTNFLPFDDQnsLVGKLRVQHPGGIVGRFPlRPwEMLEESAPVIGV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     135.61|      41|      60|     503|     548|       4
---------------------------------------------------------------------------
  503-  548 (61.85/54.04)	WKRSGVDIDSHILSFLiQDHRKSGvrffDVYHVICELVRSQSFSVA
  566-  606 (73.77/48.10)	FQQMHVSADIGLLGHL.PPHRLPS....HIWNLRNTLLSRTGFSVA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.32|      12|     607|     337|     348|       5
---------------------------------------------------------------------------
  337-  348 (20.96/13.20)	QNIVRYRKRAKR
  963-  974 (19.37/11.65)	QDLVAYRFKIAR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     265.54|      64|     223|    1157|    1220|       7
---------------------------------------------------------------------------
 1157- 1220 (103.68/55.77)	SFFFFAK....IRQRAEEQLLSLV..LSSDSLPASPVSKSG.APTSVTASALVCLRIVEDLS..................FSVPENGS.P
 1271- 1346 (67.89/33.97)	TFWFYI.............LLRLV.sIHRSTFTPGTLSKSDlADQSRLLISITCIALSKTLSpkvpsarpyyippttplmFAVPPQGSqP
 1385- 1453 (93.98/49.86)	SRFFRDRcppfLHPQNNPRLFFLLgpLAADTQPSSSTMQSS.QQQPV.APSLSQTTLGPPAS..................TATPAAGA.P
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     145.90|      48|     866|     181|     234|       8
---------------------------------------------------------------------------
  181-  234 (67.74/73.28)	DIKREAWLRDLANP.SVPLRRLSRTIpHGIrGKLLLDQCLgksIPIcRAIWLAKC
 1049- 1097 (78.16/55.72)	NLTHQALGRLLASPvQTGLAAAERTI.ESI.NDFSLPLCL...INL.RLLFSFEC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.44|      17|     201|     929|     947|      14
---------------------------------------------------------------------------
  929-  947 (27.08/21.34)	LHSDAHKNA..IPdlAELRID
 1131- 1149 (26.37/14.08)	LHADAAKEVsrIP..SELQFD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP25986 with Med12 domain of Kingdom Fungi
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