<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP25666

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMSLDGLPRHTRTVGQSELVERLVHVAYTDLQRLCNNLPGQPELDRKRELARYLHNLRQRLTRLHVLVEWAPKNRAAQIAVKCGDMMGQLRQHDMAFADAADRLYGLRQQMDWAAGPLYDTPGALDVLCNGKYSNLPRAIADVAPKPVPLETDPELIRVRDERCRRFDIEIRGLLVDERERGTMPKAMKVFSVRNGECVVGVPGEYRATLTLGGPPPLPPPPEKDEKEDGGEEKEDGGGDDDGADPPPPPPPPPPKLGGWLVVSIEMLAGEMIDADEDEGRAAHARVFPLTKTEVKILGERATARMAGMSPPPPAPPELVEGGAQGLAGLHYVCHDIALRLCAMTVMNQSKKVARGGGAWHGGAIRTEQIMGKAEAKRAESEEKDDGEKEHGETTTRRRVRGAGEGIRMWFWLPGLSGEVKASGSRMAGVDVGSVEVGLGTAAAVDAAVAAGTLPRVELTYVRADDKDSSSGAIAARALVPTATGAVETFELPFSTASVDIKEVLADGIRAAAATRLRRVLEELGPACDAAGVEGVLDEDAVNPGDADEDGWATARRPAISLRLTDAGTSVQVTCGKRGGELRVCGVASIASSGSAASVEHAACVGGIPALRGLLPTLRRYAEERDLRDAARDAGVCTHPAPRSMKGWVNGVAPAALAIVPPASGGVYVGVFVTRDGEPSGAAKSPGKSVKKSAKKKGAAAAAGGGNAGGGGGGVARLALVHAPRAHPAMAPAVASVAPLAPDGVALLDAKKRKSGGDGSFPFDSFDAVVDAVGAAVKSQRVVAQRTTVTSWLRSRGLRFIDARMASDGSSVVAFSAKLGDGAWGAEPAAACEVLLRGVHGVRLTVRGLPRAQSVRGIRGEGGASGIEARGGGEARASRGGVASIAVEADGSFVVAVDYPDGDFSPGAACGDAHRVVATHAFVGRLRPELGVGAWLEPLSAVVETSGGGAARVSWVGGIDDGGLAAVAINEESAKGEDVASEAARVGDAVAFVEALNGASR
Length1000
PositionTail
OrganismMicromonas commoda (strain RCC299 / NOUM17 / CCMP2709) (Picoplanktonic green alga)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Chlorophyta> Mamiellophyceae> Mamiellales> Mamiellaceae> Micromonas.
Aromaticity0.04
Grand average of hydropathy-0.183
Instability index41.46
Isoelectric point6.05
Molecular weight103651.12
Publications
PubMed=19359590

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP25666
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     282.55|      60|      60|     517|     576|       1
---------------------------------------------------------------------------
  370-  410 (46.46/17.07)	.......MGKA..EAKR..AESEEKDD.....G.............E.KEHG.ETTTRR.....RVRGAGE..GIRMWF..
  412-  462 (44.64/16.05)	....LPGLSGE.VKASGsrMAGV.DVG.SVEVG.................LG.TAAAVDAAVA.....AGTlpRVELTYVR
  517-  576 (101.05/47.61)	RRV.LEELGPA.CDAAG..VEGVLDED.AVNPG.............DADEDG.WATARRPAISLRLTDAGT..SVQVTCGK
  582-  637 (61.75/25.62)	RVCgVASIASS.GSAAS..VE....HA.ACVGG.............IPALRGlLPTLRRYAEERDLRDAAR..DAGV.CT.
  639-  695 (28.65/ 7.11)	.........PApRSMKG.wVNGVAPAAlAIVPPasggvyvgvfvtrDGEPSG...AAKSP.........GK..SVKKSAKK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.10|      23|      62|     242|     269|       2
---------------------------------------------------------------------------
  242-  269 (46.94/30.60)	GADPP..PPPPPPPPKL..GGwlvvsIEMLAG
  302-  328 (37.17/15.12)	TARMAgmSPPPPAPPELveGG.....AQGLAG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     336.42|      74|      75|     839|     912|       3
---------------------------------------------------------------------------
  697-  758 (64.09/17.73)	..............GAAA..........AAGGGNAGG.......GG.G...GVARLALvhapRAHPAMApavasVAP..LAPD...........GVALL....DAKKRKSGGDG
  760-  847 (85.25/25.61)	FP.FDSFDAVVDAVGAA.........VKSQRVVAQRTtvtswlrSR.GlrfIDARMA......SDGSSV.....VAFsaKLGD..GA..WGaePAAACEvllrGVHGVRLTVRG
  848-  921 (129.32/42.04)	LPRAQSVRGIRGEGGASG........IEARGGGEARA.......SR.G...GVASIAV....EADGSFV.....VAV..DYPD..GD..FS..PGAACG....DAHRVVATHAF
  922-  988 (57.75/15.36)	VGR......LRPELGVGAwleplsavVETSGGGAARV.......SWvG...G.....I....DDGGLAA.....VAI..NEESakGEdvAS..EAARVG....DA.........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      85.53|      27|      32|       3|      33|       4
---------------------------------------------------------------------------
    3-   33 (37.60/36.90)	LDGLPRHTRtvgQSELVeRLVHVAYTDLQRL
   37-   63 (47.93/31.70)	LPGQPELDR...KRELA.RYLHNLRQRLTRL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP25666 with Med14 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) EMIDADEDEGRAAHARVFPLTKTEVKILGERATARMAGMSPPPPAPPELVEGGAQ
2) GEYRATLTLGGPPPLPPPPEKDEKEDGGEEKEDGGGDDDGADPPPPPPPPPPKLGGWL
3) QIMGKAEAKRAESEEKDDGEKEHGETTTRRRV
270
203
368
324
260
399










Molecular Recognition Features






MoRF SequenceStartStop




1) KKSAKKK
690
696