<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP25226

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPTPLPLEQMPGVGGGGYLPAGQEGNRSGGNNTMSMSVLIDFIIQRTYHELTVLAELLPRKTDMERKVEIYDYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPPDAITAAEKKQTLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVALTVMGDSPNVPWRLLDIDILVEDKETGDGKALVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERLDENNITVEEYLPGVKLTVSYWRDLKSELGYRLTVQSDPSEIGRPLAVVHVPSLGAKESADVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDFNLQGTPAILTVPVLSPCLRAEQIHITIDTHTGMFRCHVPKHLDCPITDEMQDSLNGDRSKLPSLLSELRYWITHRRCEKTLQHLPATATETLPFLTLPDQELLQQGRHKIYVKLHRHPNIILVVQLKEKSHMPNEMEYTFHLGFVAYQKDEVDVIDDGAKQLVSVVAQSQSEIPKFYTKLMRLIEFDTFVATHGPGTDVDAEISPHKRKSNGDILAPPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANATSLVLKILALPQPGKTPPVVGGEANKPPTLSAPAAPLPKIESHVWEDLQRRVLSISVRSQTNKNSQVRIWVVEFVFYSTPLQSSHQKEQGSRRTVYLTYEQANHDFSKTVEDLLNDWSKIVYLYALVYDFADHIKNKRHSLTDMLVVKSYSFMNLLVGYGPKKEVSCNIYWSVQSHGFRLTFVGGMSAVNAHSMMRDQLAQHLNQQHSLTQVAHILHETYSPMSSIAKLPVLPILGVPRPNVPVLSFCMLPQSPCLMRLTYQAVYCLELRFRANRLVSIRDGASSRFERNVIEEFTPIQGLKAFLSKYVDESAMYRGRAPHEDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGAGMRGPQSPRDSGLRFPAPHTPPSSSNPHTPASPHPGAGGGGGGGGSTQGHGNFNLTSPPAPHMPHPSPGGLMPSSPLNPQPSPHMVHSPGPNTLYMPSHQDSPFTAMSPANNNWPGSPSLPRPSPRPGQSPEHKSTGGGPVGGPSGSGGGGTRGSLNRPWAGAVPTLLTHEALETICRPSPHPNKDINVTDMSPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVMLNQMHMQSLHLKVTQLPPMPDKPTFQLSPDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNMPAQVLKDFVQIMRLELKPELGGDHLKWTVQMCMRMPPSAVPIVPSGNACVVLGRLKILFFLHITKIPYNGKDWKDSPSLVLPIVYDITTNITQMAERREQVQSPMMTTASTLLRRFAEYNVQTNQCSLFPAITDLLTNLQLNTTDLPPPQPNQAIGPPVGVGVGVGSSPNPMMPMQQLQPQVGPQGPVGPGGYPQLGPNPGGPQ
Length1544
PositionTail
OrganismDrosophila willistoni (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.07
Grand average of hydropathy-0.289
Instability index57.48
Isoelectric point8.39
Molecular weight171338.93
Publications
PubMed=17994087

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP25226
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     412.30|     133|     160|     998|    1146|       1
---------------------------------------------------------------------------
  410-  461 (44.35/11.24)	......................................DFNLQGTPAIlTVPVLSP..CLRAEQIHITIDTH....TGMFRCHVPKHLDCPITdEM...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
  998- 1045 (26.01/16.96)	..................................................................................................qdslngdrsklpsllselrywithrrcektlqhlpatatetlpfltlpdqellqqgrhkiyvklhrhpniilvvqlkekshmpnemeytfhlgfvayqkdevdviddgakqlvsvvaqsqseipkfytklmrliefdtfvathgpgtdvdaeisphkrksngdilappakqqktifpayfipelahvvamcdekipfmnlaqtlskhniphsglqveanatslvlkilalpqpgktppvvggeankpptlsapaaplpkieshvwedlqrrvlsisvrsqtnknsqvriwvvefvfystplqsshqkeqgsrrtvyltyeqanhdfsktvedllndwskivylyalvydfadhiknkrhsltdmlvvksysfmnllvgygpkkevscniywsvqshgfrltfvggmsavnahsmmrdqlaqhlnqqhsltqvahilhetyspmssiaklpvlpilgvprpnvpvlsfcmlpqspclmrltyqavyclelrfranrlvsirdgassrfernvieeftpiqglkaflskyvdesamyrGRAPheddNPLSPMGMEDNFGGPSSVAGvsAGGsSPFLGA.GMRGPQSP
 1051- 1155 (184.40/100.80)	RFPAPHTPPSSSNPHTPASPHPGAGGGGGGGGSTQGhgNFNLTSPPAP.HMPHPSPgGLMPSSPLNPQPSPHmVHSPGPNTLYMPSHQDSPFT.AMSP................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aNNNWPGSP
 1156- 1231 (120.45/47.92)	SLPRPSPRPGQSPEHKSTGGGPVGGPSGSGGGGTRG....SLNRPWAG.AVP.....TLLTHEALET......ICRPSPH....P.NKDINVT.DMSP.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
 1506- 1544 (37.08/ 8.59)	........................................................................................................................................................................................................................................................................................................................................................................lerflgcvymrrqlhrniqseetltalnstepgvvlfkvdglqcqvmlnqmhmqslhlkvtqlppmpdkptfqlspddllvieqyfdtrvaappyrpnslhsicrllnmpaqvlkdfvqimrlelkpelggdhlkwtvqmcmrmppsavpivpsgnacvvlgrlkilfflhitkipyngkdwkdspslvlpivydittnitqmaerreqvqspmmttastllrrfaeynvqtnqcslfpaitdlltnlqlnttdlpppqpnqaigppvgvgvgvGSSP....NPMMPMQQLQPQVGPQGPVG..PGG.YPQLGP.NPGGPQ..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.75|      14|      23|     151|     164|       2
---------------------------------------------------------------------------
  151-  164 (26.14/17.04)	TYNRLPTCIRERIV
  177-  190 (23.62/14.66)	TLLRLNQVIQHRLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     313.93|     102|     362|     863|     985|       3
---------------------------------------------------------------------------
  863-  985 (149.04/113.75)	FVGGMsavnahsMMRDQLAQHLNQQHSLTQVAHIlhETYSPMSSIAKLP...VLPILGVPRPNVPVLSFCMLPQSPCLmRLTYQAVYCLELRF.........RANRLVSIRdgassrfeRnVIEefTPIQGLKAF
 1235- 1348 (164.90/83.45)	FLGCV.......YMRRQLHRNIQSEETLTALNST..EPGVVLFKVDGLQcqvMLNQMHMQSLHLKVTQLPPMPDKPTF.QLSPDDLLVIEQYFdtrvaappyRPNSLHSIC........R.LLN..MPAQVLKDF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.32|      16|      23|     776|     793|       4
---------------------------------------------------------------------------
  776-  793 (20.14/25.59)	VYLtYEQAnHDFSKTVED
  802-  817 (29.18/22.10)	VYL.YALV.YDFADHIKN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.47|      22|      30|     284|     306|       5
---------------------------------------------------------------------------
  279-  302 (30.87/24.34)	YNCL........hYFcQSLQLEVLYTQTLR..LN
  303-  335 (28.59/16.13)	YERLdennitveeYL.PGVKLTVSYWRDLKseLG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      75.82|      23|     367|     232|     254|       6
---------------------------------------------------------------------------
  232-  254 (39.10/26.56)	RLLDIDILVEDKETG.DGKALVHP
  593-  616 (36.72/24.45)	RLIEFDTFVATHGPGtDVDAEISP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.30|      16|      23|     678|     694|       9
---------------------------------------------------------------------------
  678-  694 (22.64/14.85)	EANATSlVLKILALPQP
  704-  719 (29.66/15.72)	EANKPP.TLSAPAAPLP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP25226 with Med14 domain of Kingdom Metazoa
Unable to open file!