<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP25221

Description Mediator of RNA polymerase II transcription subunit 13
SequenceMTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCMQADMLCVWRRVQSTKQDNTDPNALNFEITTSTKVHPPLSLAAAKELWIFWYGEEPDLSDLVDAELLRIAANQPLWNGTWKGELTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLADAATAFAAASSAQSSGSNGTTGGTTGQNQEPNTNSEPLEAPGSNGETAKASKPAHARKVMLAPFGIAAILTGNSYKATDPMAEKILEDWSSFFPLCNKDNTDVPPVVEVVSGGHKMYHPTKYVLVTDLDDMEQMEFAELDKARSASAAAAVAAAAGETAALASAAATVAVSSSSSAVAASTQAMPLATNNNNNNNNNVSATSAASAASTVSVANAAAIASRKATAPQAISALERLTFQPYYDQRPTSGFSFNTNNTHIPPSAAIEMPERSWQDCIMNTLQIDATATAAAAAAAAAAASSASIGGGGDEDEQNKPPPADSKQLVQQQIQQQQQQQQRQKLWKFVDPMQKAPCICTNAQCGGSSKRHHHHHQQQQQQQQLQPLGSPALRAAGGGNMKSSFHQHHYQRPGTPAPVSSAAVIAAAAAAASAGSHHSTSSTNTNTTNSNNNNNISSTSCSSMMTALKRHIVPFHKRLPNTLTSSSSSSLSSLFKPKTNININNNNNTTDTNIPKQRHLTAGGANAGSGSGIGGTTPHGTAHGGASQYLRNSLGGDSSMPAVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVPNLQQPPTPIDHLLDKNTPAPTPTDQHDNKSITASPYVHQTPSVEPPSYTEHSGGGGGGAGGGTASQPSLSGPGSVQPNTPTASANTPGGGSGGGGGGTTASSGGAASTIATTEPGLSGGSSQRQQSAQREHLRQCCRIHTEALHLLVFTAATMTLKDPDSYYDEEWLKEVVYDFRFQENWDYLPVKRPKLEDPSKQRRPRYSKNLYEGHTHFKPLMPSPGSVYGSQLLSLDLQAAVGGGSGISGGSGTSNDGLVGIVNSSGAGGDEPDNGNNNYFQGLDIKTEPGLLSPSCRETKSSNTGGSSSGGGNNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSTVTNTIDELKRSTAVTSAVVIAAAAAAGLANSGAGSIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTSIMATPAVAGTAATATALVPLGISSSVVGGVAIGGVLKVVKQEYNVELGSPVEEPIDDWTYVYRPPQQEKFVGSSRYAPLTNLPSQTLPALNIPPNCFYKPTWSTHKSRAASLAKAAAAQQQQQQHQKHQALQQRIQLHQQQLQMHQHQHQQQQQQQHLQQQQHQQQQQQSAGAGSSHPHQKHQQLQELLLSNAPRTPSTAMPSPSTSTVPQPLSSAGSQLLLNQLNCPQAPPGSSMQQLMRAGMSPISPGPVGVGTVPFPRSSPMHRQTPTHPPPPYPYDLAVVSPATSTSSYLNKQFNSQEPHPHSHTPQGSMHHMQGGAGGGMGIHGSGNGPNGAGSTVGGGASGNSHLSMMHYTGDAGIASGSSSAVSVPSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGGSFNPFPTHSSTSHTANSAAAASSAQRLLNGPSSSSSSSFAGGLRMISAFGGSPASSSMPGAGSGHGHGPNGGSSSSSCTPPSSNPHITGYVDDDPVDCTCGFSAVVNRRLSHRAGLFYEDELEITGIADDPGKNKQPTLLNLIQSLSRRQQGKLMPGETNSSTALEKSTGVATTGQVEQLAHAIFDLLLEQCSIIQTSSSSVHRAVQSHRRRMSRQRRLFGNSGAPAASMASIANVLEFIDAHDVISLALEQARLAFESQRMDNIMDFHGGSNQHSSSQQLTAFQAAPLALRQKLAILGSGRLTVHKWPYLPVGFTRSNKEIVRTMNSIQPMLQNAFHCKSRGGAAGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYLVVCPDNEHVASCTRSYFRELSSTYEMCKLGRHTPIRGWDGILQVSSSRSGLPNDREGPTPLDDWLRTLEHAPLAEQIRRYAVAFHHQLAPYLSRVATDKTLLNPPDGNSSSSSNIGGAGGSGSSKTGGSGQHLGNSSGTELPTDAAIKLEPGTEQQTPVSSSSGQQQQDNSQDTKPDIKGAGGANGNGSDSKPALILGDPLGVGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKVIPDSVRAQMNIQIISLEAVMELGPCGNRKRFSDEIKCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRKAYKMYTAPFVLAPMHERNDKTDFSRAAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGELLEKVCINIDVPNRLRRRKAPARYVALKKLMDFIMGLISQTPQMWRLVIGRIGRIGHSELKSWSYLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLSTPQDVTCTHILVFPTSAVCASFTRQFQNEPPVDDDFLTFEEEGNEEFSDADIGDIFWDTAHMDRVSPTGSPGRMEDNRSWPSAGGNNFKCSPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQIADDMFNQTNAHQSANDHPLDSSLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA
Length2867
PositionMiddle
OrganismDrosophila willistoni (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.06
Grand average of hydropathy-0.441
Instability index53.85
Isoelectric point6.34
Molecular weight306219.43
Publications
PubMed=17994087

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364134
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP25221
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     152.14|      25|      26|    1376|    1400|       1
---------------------------------------------------------------------------
  547-  567 (35.92/20.59)	..QLVQQQI...................QQQQQ........QQQRQKLWK
  568-  607 (24.57/11.24)	FVDPMQKAPCictnaQcggsskrhhhhhQQQQQ........QQQLQPL..
 1376- 1390 (24.81/11.44)	...............Q............QQQQQ........HQKHQALQQ
 1391- 1417 (42.17/25.74)	RIQLHQQQLQ.mhqhQ............HQQQQ........QQQH..LQQ
 1418- 1444 (24.68/11.33)	......QQHQ.....Q............QQQQSagagsshpHQKHQQLQE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     561.92|     144|     144|     650|     793|       2
---------------------------------------------------------------------------
  414-  528 (95.14/35.82)	.....................................TN.NNNNNNNNVSATSAASAASTVSvanaAAIA...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................SR.K...ATAPQAI..............SALERLtFqpyydqRPTSGFSFNTNN....THIppsaaiemP...ERSWQDCIMNTLQ...IDATATAAAAAAAAAAASSASI
  650-  773 (193.29/81.61)	.......................AASAGSHHSTSSTNTNTTNSNNNNNISSTSCSSMMTALK....RHIV...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................PF.H...KRLPNTLTS.........SSSSSLSSL.F......KPKTNININNNNNTTDTNI........P..KQRHLTAGGANAGSGSGIGGTTPHGTAHGGASQYLRNSL
  774-  894 (167.73/69.69)	GGDSSMPAVASVESPATPAPsphPNSAHSQ......PTSVPPAEQLLNMSPHAPTS.VPNLQ....QPPT...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................PIdHlldKNTPAPTPT.........DQHDNKSIT.A......SPYV.......HQTPSVEP........P..SYTEHSGGG...GGGAG.GGT.........ASQ...PSL
  895-  957 (50.31/14.90)	SGPGSVQPNTPTASANTPGG..gSGGGGGGTTASSGGAASTIATTEPGLSGGSSQRQQSAQR....EHL...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
 1521- 1589 (55.46/17.30)	......................................................................rqccrihtealhllvftaatmtlkdpdsyydeewlkevvydfrfqenwdylpvkrpkledpskqrrprysknlyeghthfkplmpspgsvygsqllsldlqaavgggsgisggsgtsndglvgivnssgaggdepdngnnnyfqgldiktepgllspscretkssntggsssgggnnlftaeglnpslndleqlfetssndecssvqihtppdsnnpsnggcstvtntidelkrstavtsavviaaaaaaglansgagsiqaedltkmfptppsheqqhpnsspcqtdvvmtdlsvdttsimatpavagtaatatalvplgisssvvggvaiggvlkvvkqeynvelgspveepiddwtyvyrppqqekfvgssryapltnlpsqtlpalnippncfykptwsthksraaslakaaaaqqqqqqhqkhqalqqriqlhqqqlqmhqhqhqqqqqqqhlqqqqhqqqqqqsagagsshphqkhqqlqelllsnaprtpstampspststvpqplssagsqlllnqlncpqappgssmqqlmragmspispgpvgvgtvpfprssPM.H...RQTPTHPPPpypydlavvSPATSTSS...........YLNKQFNSQEPHPHSHT........PqgSMHHMQGG...AGGGMGIHGSGN................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     113.78|      20|      24|    1745|    1764|       3
---------------------------------------------------------------------------
 1049- 1069 (23.15/ 6.13)	GSQLLSLDLQAAVGGGSGIsG
 1746- 1766 (34.10/13.19)	GSPASSSMPGAGSGHGHGPnG
 1767- 1784 (27.42/ 8.89)	GSSSSSCTPPSSNPHITG...
 2223- 2243 (29.11/ 9.97)	GNSSSSSNIGGAGGSGSSKtG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     114.27|      35|     645|    1136|    1191|       4
---------------------------------------------------------------------------
 1087- 1128 (55.77/17.55)	AGGDEP.........DNGNNNYFQGLDIKTEPGLLSPscretksSNTGGSS
 1138- 1181 (58.49/53.41)	AEGLNPslndleqlfETSSNDECSSVQIHTPPDSNNP.......SNGGCST
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.40|      21|      24|     256|     279|       5
---------------------------------------------------------------------------
  259-  279 (36.60/22.95)	TNSEPLEAP.G....SNGETAKASKP
  281-  306 (25.80/ 6.59)	HARKVMLAPfGiaaiLTGNSYKATDP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      44.65|      14|      15|     371|     385|       6
---------------------------------------------------------------------------
  371-  384 (22.66/ 8.24)	ASAAAAVAAAAGET
  388-  401 (21.99/ 8.91)	ASAAATVAVSSSSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.17|      10|      24|    1701|    1710|       7
---------------------------------------------------------------------------
 1701- 1710 (19.39/ 9.72)	PTHSSTSHTA
 1726- 1735 (17.78/ 8.12)	PSSSSSSSFA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.60|      11|      31|     585|     595|       8
---------------------------------------------------------------------------
  585-  595 (24.17/12.06)	GGSSKR..HHHHH
  617-  629 (19.43/ 8.23)	GGNMKSsfHQHHY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.99|      13|      24|    1461|    1474|       9
---------------------------------------------------------------------------
 1461- 1474 (20.16/12.26)	PSTSTVPQpLSSAG
 1488- 1500 (23.83/ 9.49)	PPGSSMQQ.LMRAG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      75.55|      23|      24|    1277|    1299|      10
---------------------------------------------------------------------------
 1263- 1287 (25.01/10.88)	AVAGTAATATAL..vpLGISSSVVGGV
 1288- 1312 (26.22/11.76)	AIGGVLKVVKQEynveLG..SPVEEPI
 1314- 1331 (24.32/10.38)	DWTYVYRPPQQE....KFVGSS.....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.34|      16|     167|    1192|    1207|      12
---------------------------------------------------------------------------
 1192- 1207 (25.66/12.87)	STAVTSAVVIAAAAAA
 1360- 1375 (25.68/12.88)	STHKSRAASLAKAAAA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     119.63|      37|    1346|     308|     345|      13
---------------------------------------------------------------------------
   48-   96 (52.97/31.63)	ADPILRSYSRCMQadmLCvwrrvqstKQDNTD.PNALNFeITTSTKVHPP
  308-  345 (66.66/46.41)	AEKILEDWSSFFP...LC........NKDNTDvPPVVEV.VSGGHKMYHP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP25221 with Med13 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AAAASSASIGGGGDEDEQNKPPPADSKQLVQQQIQQQQQQQQRQKLWK
2) ALKRHIVPFHKRLPNTLTSSSSSSLSSLFKPKTNININNNNNTTDTNIPKQRHLTAGGANAGSGSGIGGTTPHGTAHGGASQYLRNSLGGDSSMPAVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVPNLQQPPTPIDHLLDKNTPAPTPTDQHDNKSITASPYVHQTPSVEPPSYTEHSGGGGGGAGGGTASQPSLSGPGSVQPNTPTASANTPGGGSGGGGGGTTASSGGAASTIATTEPGLSGGSSQRQQSAQREHLRQ
3) ASSSMPGAGSGHGHGPNGGSSSSSCTPPSSNPHITGY
4) CTNAQCGGSSKRHHHHHQQQQQQQQLQPLGSPALRAAGGGNMKSSFHQHHYQRPGTPAPVSSAAVIAAAAAAASAGSHHSTSSTNTNTTNSNNNNNISSTSCSS
5) DRVSPTGSPGRMEDNRSWPSAGGNNFKCSPPQEVE
6) GLLSPSCRETKSSNTGGSSSGGGNNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSTVTNTID
7) GSGISGGSGTSNDGLVGIVNSSGAGGDEPDNGNNNYFQGLD
8) KNKQPTLLNLIQSLSRRQQGKLMPGETNSSTALEKSTGVA
9) QAEDLTKMFPTPPSHEQQHPNSSPCQTDVV
10) RVATDKTLLNPPDGNSSSSSNIGGAGGSGSSKTGGSGQHLGNSSGTELPTDAAIKLEPGTEQQTPVSSSSGQQQQDNSQDTKPDIKGAGGANGNGSDSKPAL
11) SRAASLAKAAAAQQQQQQHQKHQALQQRIQLHQQQLQMHQHQHQQQQQQQHLQQQQHQQQQQQSAGAGSSHPHQKHQQLQELLLSNAPRTPSTAMPSPSTSTVPQPLSSAGSQLLLNQLNCPQAPPGSSMQQLMRAGMSPISPGPVGVGTVPFPRSSPMHRQTPTHPPPPYPYDLAVVSP
12) SSAQSSGSNGTTGGTTGQNQEPNTNSEPLEAPGSNGETAKASKPA
13) TSSYLNKQFNSQEPHPHSHTPQGSMHHMQGGAGGGMGIHGSGNGPNGAGSTVGGGASGNSHLSMMHYTG
520
686
1749
579
2705
1110
1064
1828
1218
2210
1364
236
1547
567
959
1785
682
2739
1187
1104
1867
1247
2311
1543
280
1615










Molecular Recognition Features






MoRF SequenceStartStop




1) RHIVPFHKRL
689
698