<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP24952

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDDVLNTQFARVEKALTTLVDSIAAYNPAPQAAIDLFTADDELSEGLDQLAKHQANHVRIQLLRAEADALEEQLKNSVKKLAELRHDLFNTPVTTFPQDSRAVPFDELLQYATNISRYTVPPTYREPVPVPDKDKEEEDVASSSLPTNGLNTPAIVPETTDPANDGLQAQKEGGDADAPAPEVTPEEEEWLKKLKASNLPWYPWPNNDRIRAGNLYSLQYWREKGKDLDTFNIPAYMEEERLKALGQNVESKASPAKEPEEHRDRGAPRPAIKLGVFTGLDDMED
Length285
PositionMiddle
OrganismPyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Dothideomycetes> Pleosporomycetidae> Pleosporales> Pleosporineae> Pleosporaceae> Pyrenophora.
Aromaticity0.07
Grand average of hydropathy-0.743
Instability index50.44
Isoelectric point4.58
Molecular weight31843.95
Publications
PubMed=23316438

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP24952
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.60|      17|      23|     139|     160|       1
---------------------------------------------------------------------------
  127-  147 (24.47/ 6.83)	PVPVPdkdkEEEDVASSSLPT
  153-  169 (31.13/22.37)	PAIVP....ETTDPANDGLQA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     135.07|      43|     106|      66|     111|       2
---------------------------------------------------------------------------
   66-  111 (64.33/46.98)	EADA.LEEQLKNSVKKLAELRHDlfNTPVTTFPQDSRaVPFDEL..LQY
  175-  220 (70.74/41.43)	DADApAPEVTPEEEEWLKKLKAS..NLPWYPWPNNDR.IRAGNLysLQY
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP24952 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) EERLKALGQNVESKASPAKEPEEHRDRGAPRPAIKLGVFT
2) VPPTYREPVPVPDKDKEEEDVASSSLPTNGLNTPAIVPETTDPANDGLQAQKEGGDADAPAPEVTPEEEEW
239
120
278
190










Molecular Recognition Features






MoRF SequenceStartStop




1) EEEEWLKKLK
2) ELLQYATNISRYTV
3) IRAGNLYSLQYWR
4) KEPEEHRDRGAPRPAIKLGVFTGLDDMED
5) LPWYPW
186
107
210
257
199
195
120
222
285
204