<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP24924

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMASLNLLPEDLKHLDLLRNRFATLSLNLSNAHRNMALTYPLPSQESLQASAAIIHTSLLSLQSILTDKSALFHRIAVHPSTNFPGRTQLDFLSSMLRKKPEPEIETKMEMGMQRAREVGVDEAVLAEIARRNKRREGGDDEDYEEGGGGGGGDGDEGEDEEANNEKWADCWFLFDRGLKEYINVQEGRSYTVEEQEMGVERVRTGLRRNLLEEEEEEEEESDDEEEDEDEEEDEDLVMMDGSGGGRGGVGQQGVVQQASGNTGVQPEHLFWLYARGRTDLPPRIELESKRAPAKGQVKRLPPR
Length303
PositionHead
OrganismPodospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Sordariales> Chaetomiaceae> Podospora> Podospora anserina.
Aromaticity0.05
Grand average of hydropathy-0.858
Instability index70.60
Isoelectric point4.59
Molecular weight34008.12
Publications
PubMed=18460219
PubMed=24558260

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP24924
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      77.99|      17|      18|     141|     157|       1
---------------------------------------------------------------------------
  141-  157 (34.64/14.05)	EDYEEGGGGGGGDG....DEG
  158-  177 (22.50/ 6.97)	ED.EEANNEKWADCwflfDRG
  214-  227 (20.85/ 6.00)	EEEEEEE...SDDE....EED
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.58|      15|      17|      41|      55|       3
---------------------------------------------------------------------------
   41-   55 (24.46/16.76)	LPSQESLQASAAIIH
   59-   73 (24.13/16.45)	LSLQSILTDKSALFH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      33.58|      11|      18|     184|     194|       6
---------------------------------------------------------------------------
  184-  194 (19.23/10.52)	VQEG.RSYTVEE
  202-  213 (14.34/ 6.29)	VRTGlRRNLLEE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP24924 with Med8 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ARRNKRREGGDDEDYEEGGGGGGGDGDEGEDEEANNEK
2) RVRTGLRRNLLEEEEEEEEESDDEEEDEDEEEDEDLVMMDGSGGGRGGVGQQGVVQQASGN
129
201
166
261










Molecular Recognition Features






MoRF SequenceStartStop




1) VQPEHLFWLYARGRTDLPPRIELESKRAPAKGQVKRLPPR
264
303