<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP24889

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMMHSANGKQQKIEQKVGGPTKMPNSTGTAGPGTAAGIGDKHESWQMELLMERLRSKAKSSHYKSFPEMSKTVRMSLLVRSVKSFVLKLSEDTSGLFISSDMFYLEIILDQNGKVQDVKVHHECKMKQQSCSELVSCLQRGDFVDFTTQLEANNIAKGLFVTLPDQHHCYYMNENKNLEGIVVSSIPFTEPQHVSKILVFLRQQALFNQLLSSCIRNNSSTTRTTDHDQLLVFEVTALSCQYITISLEHPFDESLAMVEFDLRNSLAVQVRVFCNGYESDERISKHISIVVQRTMSIPVTLRALIKIWEEDFEAKFKGKLSSNAGGNGCIDEVNFSLPMGPDDGGGSGPGGGGGGNGLGMNGQHTTGINGSGSSHQEFVDAKKIKYETQDLSKKRRVGEDFRNSPKSNNMEKSLKSEVNVADDDDCDESNNSDSYNSLGGNQTTTTTQHVSSSSASKSSSGKHTPTSPFALGIGEDGFRNKYTGKMNSLNSSPDTILKNSETTDCLEPTKAINQTSASNPEDSNLTVSVTTIEVGKQNMSSSSVLSNINLDKRPGIEIIPLSTSATSIPSSITITPIPVENCAGPSKNSSSDKKLSGSSSSSSSSNRKNSDSKTESDRAKLEKKKKRKHEEHQSQLQMGPPHKILSKNVSTDGPTSPSGGSRRISISPVPPKSGSSSSPRHSPVHHSSPKHQGSYGTSSPKHISGGSSGGGKPSMSALKSAASGGSPNSKSSSGSSTGKDEGSGVFSTGNTSKSSSSSGSSSSRDRDRDRERSDKKSVSNPKLKTPAVKLKQLDLATCGASLSAGVQLELINSNSSGGSSTGSSSIDYTDNSLFASSDMSKNSSVAAASTAGMLLLQQAMKNRKSSLSAVIDKLKSAQSGDESSLMLLPELAQQAGFTLKESSGSNKVASSSSASPTTMVSNIHGHTSSSASATGASSLTNTVAALDTQLSSTTSPVHGKSSEYLVKPSSDGMKLTIQNKKGSKSSLSSSSTNSKSSSKSGLKPGVSSGPGSKKLQSSQSFSGSFSSVSSSSSKSGSSTKSSFQKSNSSSSLSNSSGSLKSSSKSGSSSSSSGSSSKDKARNSSKSSSLISSNAMTLASQAAANAAVNANALNVMKMLGITSSIQNMEGFVKTLDTKFQIPKLSARSASSGNDLDKIKELRAVSAPTTPLQNMSQSPTGINDFSLPFNKLPGDVSPLHSAMLPNVMQKMFAGHDNAMLLRRSPNLEHQASNDGFPSQHGKGSTPPTPTISIPPPFPSPVGLQSHAMDFSNSSDSIDNSNLMLPPTRPSSTMSNHSSSDKLIDATGGNNSMDSFSSSPSAQAIAAAAAQFINKLDNTQQLVAALKNHHLNALSNAAAAAACSSAGSGSVTSPTSVSVHIMKSPAPSPLGMPGNSVDDDLMDDTLVQIGGK
Length1408
PositionMiddle
OrganismCulex quinquefasciatus (Southern house mosquito) (Culex pungens)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Nematocera> Culicoidea> Culicidae> Culicinae> Culicini> Culex> Culex.
Aromaticity0.04
Grand average of hydropathy-0.549
Instability index55.09
Isoelectric point9.17
Molecular weight147401.80
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP24889
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      98.83|      27|      28|    1015|    1041|       1
---------------------------------------------------------------------------
  984- 1024 (32.18/ 7.37)	SSLS...SSSTNSKSSSKSGlkpgvssgpgskklQSSQSF...SGSF
 1025- 1058 (34.06/ 8.34)	SSVS...SSSSKSGSSTKSS..........fqksNSSSSLsnsSGSL
 1059- 1088 (32.59/ 7.58)	KSSSksgSSSSSSGSSSKDK..............ARNSSK...SSSL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     559.38|      83|      83|     672|     754|       2
---------------------------------------------------------------------------
  321-  367 (49.52/11.09)	....................................................SNA.....G..........GNGCIDE....VNF....SLPMG..........PDD.........GGG.SGPGGGGG.GNGL...G......MN...GQHTTGI
  368-  450 (70.02/19.10)	NGS.GSS..HQ......EfVDAkkikyetqdlSKKRRVGEDFRNSPK.....SNN.....M..........EK....S....LKSevnvADDDDC.........DES.........NNSdSYNSLGGNQ..............TT...TTTQHVS
  453-  540 (65.49/17.33)	SASkSSSGKHT......P.........................TSPFALGIGEDGfrnkyT..........GK..MNS....LNS....SPDTIL.........KNSettdcleptKAI.NQTSASNPEDSNLtvsV......TTievGKQNMSS
  561-  625 (69.50/18.90)	STS.ATSIPSSititpiP.VEN..........CAGPSKNS...SSDKKLSGSSS..............................S....SSSSNR.........KNS.........DSK.TESDRAKLE......................KKKK
  633-  676 (53.12/12.49)	SQL.QMGP.........P..HK..........ILSKNVSTDGPTSP...SGGSR..................RISISP....V...........P.........PKS.........GSS.S..................................
  677-  754 (130.91/42.90)	......SPRHS......P.VHH..........SSPKHQGSYGTSSPKHISGGSSG.....G..........GKPSMSA....LKS....AASGGS.........PNS.........KSS.SGSSTGKDEGSGV...F......ST...GNTSKSS
  757-  819 (60.59/15.41)	SGS.SSSRDRD......R.DRE..........RSDKK....SVSNPK......LK.....T..........PAVKLKQ....LDL....ATCGASlsagvqlelINS.........NSS.GGS...............................S
  820-  901 (60.23/15.27)	TGS.SS.............IDY..........TDNSLFASSDMSKNSSVAAASTA.....GmlllqqamknRKSSLSAvidkLKS....AQS.................................G.DESSLM...LlpelaqQA...GFTLKES
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      88.85|      23|      28|    1250|    1272|       3
---------------------------------------------------------------------------
  925-  951 (21.95/ 6.24)	HTSSSASATGASSltntvA........ALD.TQLSS
 1250- 1272 (41.20/19.28)	IPPPFPSPVGLQS.....H........AMDFSNSSD
 1281- 1310 (25.69/ 8.78)	LPPTRPSS.TMSN.....HsssdklidATGGNNSMD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.61|      12|      28|     972|     983|       4
---------------------------------------------------------------------------
  972-  983 (20.30/ 9.41)	MKLTIQNKKGSK
 1001- 1012 (21.30/10.28)	LKPGVSSGPGSK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.92|      14|      15|    1321|    1334|       5
---------------------------------------------------------------------------
 1321- 1334 (22.56/15.33)	IAAAAAQFINKLDN
 1339- 1352 (23.36/16.14)	VAALKNHHLNALSN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      87.92|      22|      47|    1120|    1142|       6
---------------------------------------------------------------------------
 1120- 1142 (34.12/23.75)	TSSIQNMEGFVKTLDtKFQIP..KL
 1146- 1159 (20.81/ 8.44)	SASSGN......DLD...KIK..EL
 1166- 1189 (32.99/18.06)	TTPLQNMSQSPTGIN.DFSLPfnKL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.91|      13|      18|    1199|    1213|      10
---------------------------------------------------------------------------
 1199- 1213 (20.73/19.11)	AML....PNVMQKmfAGHD
 1215- 1231 (19.18/ 9.62)	AMLlrrsPNLEHQ..ASND
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP24889 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DEVNFSLPMGPDDGGGSGPGGGGGGNGLGMNGQHTTGINGSGSSHQEFVDAKKIKYE
2) GHDNAMLLRRSPNLEHQASNDGFPSQHGKGSTPPTPTISIPPPFPSPVGLQSHAMDFSN
3) GSSTKSSFQKSNSSSSLSNSSGSLKSSSKSGSSSSSSGSSSKDKARNSSKSSSLIS
4) MMHSANGKQQKIEQKVGGPTKMPNSTGTAGPGTAAGI
5) NKYTGKMNSLNSSPDTILKNSETTDCLEPTKAINQTSASNPEDSNLTVSVTTIEVGKQNMSSSSVLSNINLDKRPGIEIIPLSTSA
6) PKLSARSASSGNDLDKIKELRAVSAPTTPLQNMSQSPTGINDFSL
7) PTSVSVHIMKSPAPSPLGMPGNSVDDDLMDDT
8) QDLSKKRRVGEDFRNSPKSNNMEKSLKSEVNVADDDDCDESNNSDSYNSLGGNQTTTTTQHVSSSSASKSSSGKHTPTSPFALGIGEDG
9) SDSIDNSNLMLPPTRPSSTMSNHSSSDKLIDATGGNNSMDSFSSSP
10) SIPSSITITPIPVENCAGPSKNSSSDKKLSGSSSSSSSSNRKNSDSKTESDRAKLEKKKKRKHEEHQSQLQMGPPHKILSKNVSTDGPTSPSGGSRRISISPVPPKSGSSSSPRHSPVHHSSPKHQGSYGTSSPKHISGGSSGGGKPSMSALKSAASGGSPNSKSSSGSSTGKDEGSGVFSTGNTSKSSSSSGSSSSRDRDRDRERSDKKSVSNPKLKTPAVKL
11) SSGSNKVASSSSASPTTMVSNIHGHTSSSASATGASS
12) VAALDTQLSSTTSPVHGKSSEYLVKPSSDGMKLTIQNKKGSKSSLSSSSTNSKSSSKSGLKPGVSSGPGSKKLQSSQS
330
1211
1035
1
479
1140
1370
388
1271
566
901
942
386
1269
1090
37
564
1184
1401
476
1316
789
937
1019










Molecular Recognition Features






MoRF SequenceStartStop




1) KKKKRKH
2) SMSALKS
622
713
628
719