<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP24774

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMVIDSYVETLDTIIRLFQEYKPGSITLDNITKLCQTLGLESFIDDIDSHLSRLSTASKIIVVDIDFNKIEGKVVDVKLVLASNFDNFNYFNDENGKGDNILLNSLTQYSTLREFHHNLQYIYLLDTYSQIDSDPSNSNNGTANSTGANSTGLLNVDGNTSAATPAISVSASTTGNGKLDLFKYYTELSEFMKNYFQANCIDLKIETNSGNIFGIYIVSPDDNCKLAKIYLEKSKDPSHKLFEYVFSDDTKCWINENAENYTTGVSLVMEIIDSDDSNIWFPKEFISPELICEQDKNSRNGFSNDLNKENNLDANKNNILSLFPNNISEFSYNERVQVINDFTTDLINISKFNIGNDNLDLILEIINWIKWYKIVLTKIYDTLSHTDNNSGIFNSKNGFQNDNINNKSMNIENNTESNNINGRRRSSSIGSTRKRRSSTKSKRPIIAEAMMLKEEGLQQFNLHEILSEPAVEDETEAPATNGETLLSKNDDMDTTSTSTSTSTSTSTSEKGQSNVILCEDHVSLDQTSSCTLYDDVEKWNSFIDIFKKHIKKANDDNN
Length557
PositionMiddle
OrganismVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Vanderwaltozyma.
Aromaticity0.09
Grand average of hydropathy-0.537
Instability index39.94
Isoelectric point4.62
Molecular weight62762.64
Publications
PubMed=17494770

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364059
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP24774
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.97|      19|      21|     133|     151|       2
---------------------------------------------------------------------------
  133-  151 (34.06/16.97)	DPSNSNNGTANSTGANSTG
  156-  174 (31.91/15.51)	DGNTSAATPAISVSASTTG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     105.44|      34|     178|     175|     212|       3
---------------------------------------------------------------------------
  175-  212 (51.71/38.78)	NGKLDLFkyyTELSEFMKNY..FQANCIDL..KIETNSGnIF
  355-  392 (53.72/28.27)	NDNLDLI...LEIINWIKWYkiVLTKIYDTlsHTDNNSG.IF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      74.29|      26|     493|      25|      52|       6
---------------------------------------------------------------------------
   25-   52 (37.86/33.39)	ITLDNiTKLCqTL..GLE...SFIDDIDSHLSR
  521-  551 (36.43/22.33)	VSLDQ.TSSC.TLydDVEkwnSFIDIFKKHIKK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      78.87|      22|      25|     231|     252|       7
---------------------------------------------------------------------------
  231-  252 (40.97/21.23)	EKSKDPSHKLFEYVFSDDTKCW
  258-  279 (37.90/19.21)	ENYTTGVSLVMEIIDSDDSNIW
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP24774 with Med1 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) HEILSEPAVEDETEAPATNGETLLSKNDDMDTTSTSTSTSTSTSTSEKGQSNVILC
2) NINNKSMNIENNTESNNINGRRRSSSIGSTRKRRSSTKSKRPI
462
402
517
444










Molecular Recognition Features






MoRF SequenceStartStop




1) VEKWNSFIDIFKKHIKKAND
535
554