<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP24763

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMSTEVDASPLNGGGFAIENGGSHGAVGEYEGSSGGDATAAAAAAAAAAADVEVNEAPTDADADAGADVDANVDVKVDGTATTANEVVDEKSDLGKVVVEPPALPHVEINQLPLSLVIRNLTVFTIKEISQYMKTNVHVNQQEGSSGKKLKFLQLIIFLRNQFLKLYVLVKWCRTIKNNNFHTLIDLLNWFRTTNMSVNSCIWALKNNLGAMANAKLPNADLITALEVLSLGRPDLPTHNYQLSGEDDPNQLKNGMTKIPPKLILRRLYDLNLNVSIKISWMEFPEQFGNYQIKDGRIYITVNNEFELQLSTVDRYSPLFFVNLKFLFDNQNLPMNKPRLEKYINDLLYKSNKPLFAVYTFLHKYVLTLQLYMIHIELSGLENGGKFFGGNLVHRYDIKKSVITVKYWLNVRMGNRGLITIGIDKLTENLILRWENQNTSDSTVIPKVYTNILSNLESILDEIMYNHAHMIRMGLLSKGIFQEDEENSSVLLFQIPTTCLSVAPVQLKIDLISGIFYFKNPTPLLQSYAQQMNKAESAEELTVILQRLRLDKITQILRNMFEKTGWVCSKVVKLSQPIKTQFDVDKSSDNSSTLLQNDMFISLPKWPTHWYLILTIISSNSFCIVEKRIGKIMAIKGKWELKYLDESSLIASKLETITYQKIMNLQRSILHKIINHMIIDSLNQLKISNRVCSQEAIEKALPDYIISDLDSENSLDSFRTSLPAYSSIIALELNSLLNISNTLGNVFETSLFLQIDYARSEIKLYGKFKFDTMVLKSKCDELKINFIPSDPLAFYLCEKFTNLNVTVQYLTKFKKILMQLIILTDVVERLRKNFDTEHFKILDLKPNEVSFKYFKNDLDEPDCRINIITNDEMIENLKVELSPSNPQSIIQPFIDNNGMDYHFIFHYLQFTSSLFTTFNTILSDEKGGMYKNTYVQLGLHNLNEYQILYFNPETGAKIGVLIELKSVPRNGEQKVQYYVHFSDENISAKSPAYQLVQEVRNKVFKLDNDSLPSSRHKNTIRLSSGIACDANAIGPVLSDIHNILKIDSIEQSTSKVSTNEIKDAIIV
Length1066
PositionTail
OrganismVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Vanderwaltozyma.
Aromaticity0.09
Grand average of hydropathy-0.138
Instability index35.75
Isoelectric point6.06
Molecular weight120873.58
Publications
PubMed=17494770

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
TFIIB-class transcription factor binding	GO:0001093	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP24763
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     499.61|     113|     389|     479|     591|       1
---------------------------------------------------------------------------
  479-  562 (119.62/77.54)	............................................IFQED..............E..ENSSV.LL....F....QIPT......TCLSV..APVQL......KIDLI.SG.....I.F.Y..FKNPTPLLQSYA............................QQMNKAESAEELTVILQRLRLDKIT..QILRNMFE.........K
  563-  671 (107.45/68.82)	TGWVCSKVV...K..L.SQP....IKTQFDVDKSSDNSS................................tLLqndmFislpKWPThwylilTIISS..NSFCIvekrigKIMAI.KG.....K.W.E..LK........YL............................DESSLIASKLE.TITYQ.....KIM..NLQRSILH.........K
  687-  844 (83.42/51.60)	SNRVCSQEAiekA..L.PDY....IISDLDSENSLDS............frtslpayssiialE..LNS....L....L....NISN......TLGNVfeTSLFL......QIDYArSE.....IkL.YgkFKFDTMVLKSKCdelkinfipsdplafylcekftnlnvtvQYLTKFKK.....ILMQLIILTDVV..ERLRKNFDtehfkildlK
  845-  952 (97.69/61.82)	PNEVSFKYF...KndL.DEP...................dcrinIITND..............EmiENLKV.EL..........SPS......NPQSI....IQP......FID.N.NGmdyhfI.FhY..LQFTSSLFTTFN............................TILSDEKGGMYKNTYVQ.LGLHNLNeyQIL..YFN........pE
  953- 1050 (91.43/57.34)	TGAKIGVLI...E..LkSVPrngeQKVQYYVHFSDENIS.akspAYQLV..............Q..EVRNK..V....F....KLDN......DSLPS..SRHKN......TIRLS.SG.....I.A.C..DANAIGPVLSDI............................HNILKIDSIEQ..................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.17|      19|      20|      36|      54|       2
---------------------------------------------------------------------------
   36-   54 (29.76/22.56)	DATAAAAAAAAAAADVEVN
   59-   77 (32.41/25.18)	DADADAGADVDANVDVKVD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     260.51|      87|     103|     230|     331|       3
---------------------------------------------------------------------------
  230-  331 (132.24/122.96)	LGRPDLPTH.NYQLSGEDDPnqLKNGMTkippklILRRlYDLNLN...VSIKISWMEFPEQF..GN....YQIKDGriYITVNNEFELQLST.......VDRYSPlffvNLKFLFDNQN
  334-  437 (128.27/82.13)	MNKPRLEKYiNDLLYKSNKP..LFAVYT......FLHK.YVLTLQlymIHIELSGLENGGKFfgGNlvhrYDIKKS..VITVKYWLNVRMGNrglitigIDKLTE....NLILRWENQN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.09|      15|      19|     160|     177|       4
---------------------------------------------------------------------------
  160-  174 (29.42/27.11)	NQFLKLYVLVKWCRT
  178-  192 (29.67/16.65)	NNFHTLIDLLNWFRT
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP24763 with Med14 domain of Kingdom Fungi
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