<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP23128

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMQREEKQLEASLDALLSQVADLKNSLGSFIYKLENEYDRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPADTNALVAAVAFGKGLSNWRPAGSSGPSQPGQPGAGTVLAGASGLQQVQMAGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQRSPPPSTLGISTKNSSSLWWPHSSWALESSACCSGSVSYV
Length307
PositionHead
OrganismBos taurus (Bovine)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Artiodactyla> Ruminantia> Pecora> Bovidae> Bovinae> Bos.
Aromaticity0.05
Grand average of hydropathy-0.511
Instability index50.52
Isoelectric point6.54
Molecular weight33257.12
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364144

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP23128
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      71.03|      14|      17|     218|     231|       1
---------------------------------------------------------------------------
  200-  213 (23.01/ 8.51)	SGPSQPGQPGAGTV
  218-  231 (25.70/10.19)	SGLQQVQMAGAPNQ
  237-  249 (22.32/ 8.08)	SGVQMAQ.AGQPGK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      76.18|      25|      28|      67|      92|       2
---------------------------------------------------------------------------
   67-   92 (40.28/26.97)	KTPLFRNQViIP..LVLSPDRD.EDLMRQ
   96-  123 (35.90/19.61)	RVPVFSHEV.VPdhLRTKPDPEvEEQEKQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.08|      16|      20|     251|     266|       3
---------------------------------------------------------------------------
  251-  266 (30.70/18.22)	PS..GIKTNIKSASMHPY
  272-  289 (28.38/16.37)	PStlGISTKNSSSLWWPH
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP23128 with Med8 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADA
2) LEKISKEERESESGGLRPNKQTFNPADTNALV
3) LSNWRPAGSSGPSQPGQPGAGTVLAGASGLQQVQMAGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQRSPPPSTL
100
151
191
135
182
275










Molecular Recognition Features






MoRF SequenceStartStop




NANANA