<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP22900

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMPYMGESIREEVEVALHEHRFLVTKLLESLNTASLPPSTITATRLRTVTETFQKVLAKDRDLLAAVKKLSRHQVAQTELLRIKTEIANKKQKVVEYATQLHRSQENVAQVLRKHRVVLQNTKEKAKVVLDPRDLIAYAHRIAGTTSAPKEWQPGFPMFGFMPPAPQEHMMRAGVLSRGIVAEIVTQGPELYTSIAARVKQEGDGHEDIEKLDIPIGLGASDAQIRKQMPPGWKPGDPVDLPLDALLHYMGRNFFTEHGITLPESWKMGDALPTDAMEILRKKFKLPEKRAALYDDEVVDDVETLRKKRKLEEGEKADGGTAESESSSDSSDSDEEAPNTISLSLSSSEDDSDSD
Length354
PositionMiddle
OrganismPeronospora effusa
KingdomOomycetes
LineageEukaryota> Sar> Stramenopiles> Oomycota> Peronosporales> Peronosporaceae> Peronospora.
Aromaticity0.05
Grand average of hydropathy-0.551
Instability index51.86
Isoelectric point5.60
Molecular weight39481.35
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP22900
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.05|      20|      22|     314|     333|       1
---------------------------------------------------------------------------
  314-  333 (34.66/19.17)	EKADGGTAESESSS.DSSDSD
  334-  354 (30.39/16.10)	EEAPNTISLSLSSSeDDSDSD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     155.38|      43|      79|     148|     192|       2
---------------------------------------------------------------------------
  148-  192 (78.88/60.36)	PKEWQPGFPMfgFMPPAPQEHMM.RAGVLSRGI.VAEIVTQGPELYT
  229-  273 (76.50/51.37)	PPGWKPGDPV..DLPLDALLHYMgRNFFTEHGItLPESWKMGDALPT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      52.30|      11|      71|      54|      64|       3
---------------------------------------------------------------------------
   54-   64 (18.00/ 9.84)	KVLAKDRDLLA
  109-  119 (16.98/ 8.93)	QVLRKHRVVLQ
  126-  136 (17.32/ 9.23)	KVVLDPRDLIA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.45|      14|      24|     274|     287|       4
---------------------------------------------------------------------------
  274-  287 (25.89/18.39)	DAMEILRKKFKLPE
  299-  312 (24.57/17.10)	DDVETLRKKRKLEE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP22900 with Med4 domain of Kingdom Oomycetes


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) TLRKKRKLEEGEKADGGTAESESSSDSSDSDEEAPNTISLSLSSSEDDSDSD
303
354










Molecular Recognition Features






MoRF SequenceStartStop




1) ILRKKFKL
2) SDSDEEAPNTISLSLSSSEDDSDSD
3) TLRKKRKLEEGEK
278
330
303
285
354
315