<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP21853

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDEMIDHRFERVEKALAALINSISTYNPSPTLANDLVAADAGLNEGLEQLSTHQSNYAKILSLRANSQELDGQIRDTLTLLTTTRSALVSVASTSFPDTTNPISYSELLSYARRISKFTLPSTYREPEARPGSADAGYATNANTPKESKSETQTNGSTTPVGVTNGVDMDTQMTVGTAMDIDNGNGNGTPAAGAQQTSQATNATSSTALPNEFSQWLNPAADLPFTPWPSEETIRRGALASLQILMDKGIDPATFDPEKSAELEAERKRIMEEEDRLREEEKTRIEEERRREMERRASISAGLGEERREERREEKPSVFQLETFDDDDED
Length330
PositionMiddle
OrganismPhialophora cf. hyalina BP 5553
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Leotiomycetes> Helotiales> Pleuroascaceae> Venustampulla.
Aromaticity0.05
Grand average of hydropathy-0.781
Instability index49.47
Isoelectric point4.55
Molecular weight36387.42
Publications
PubMed=30018880

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP21853
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      86.46|      25|      28|     155|     182|       1
---------------------------------------------------------------------------
  155-  179 (44.52/33.79)	NGSTTPVGVTNGVDMDTQMTVGTAM
  185-  209 (41.93/22.88)	NGNGTPAAGAQQTSQATNATSSTAL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      60.34|      12|      15|     285|     296|       2
---------------------------------------------------------------------------
  263-  274 (19.50/ 9.91)	LEAERKRIMEEE
  285-  296 (23.01/12.89)	IEEERRREMERR
  303-  312 (17.83/ 8.50)	LGEERREE..RR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      72.93|      23|      28|      40|      62|       3
---------------------------------------------------------------------------
   40-   62 (37.57/22.24)	DAGLNEGLEQLSTHQSNYAKILS
   71-   93 (35.35/20.58)	DGQIRDTLTLLTTTRSALVSVAS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.16|      13|      30|     217|     232|       4
---------------------------------------------------------------------------
  217-  232 (21.78/20.41)	LNPAAdlpFTPWPSEE
  250-  262 (23.37/12.35)	IDPAT...FDPEKSAE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP21853 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LPSTYREPEARPGSADAGYATNANTPKESKSETQTNGSTTPVGVTNGVDMDTQMTVGTAMDIDNGNGNGTPAAGAQQTSQATNATSSTALPNEFSQWLNPAADLPFTPWPSEETIRRGALASLQILMDKGIDPATFDPEKSAELEAERKRIMEEEDRLREEEKTRIEEERRREMERRASISAGLGEERREERREEKPSVFQLETFDDDDED
120
330










Molecular Recognition Features






MoRF SequenceStartStop




1) ISYSELLSY
2) REERREEKPSVFQLETFDDDDED
103
308
111
330