<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP21357

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMPGVVMDNANIGGLRHGPGNTYPQDGLSHPRGEVAQFNGANAQDGPVYVNGVEKNASQTRSVETVSANHASRVTKGPPELPHITQGFFPFAKLVNRSVQQCWNDLSDLITEMAEMQVNSHEFHPPTAPTTGKSPGNQSPGNIRKKLRILEFSHAKRAEFIKLLVLSQWSRQAADVSKLIDIQNFIRTRHQAYMNALQWVGDMKRDLVQAQVANPDLKTALEVLSRGKVASMSDFGYKPPRRLTVRGTLRKLQKINRLIGARLVVQEEIPPPFRDYRVHDGRVTFSVPGEFELDLSIGEENENSQFFFVDIRFLFSPSPPVLKGRLLNELEMTINDVLQNSGLTGCFDLLHNLVLTNKVNILFKQATELARGSWSEGLRVELLHRTLVVQYWTLKPGAKSWLEIGIRRGHRKSDSESPGLPSLKFRWMRDGGEVPCEDIAFDAENLSMEVVLRSVIALHVSHILSSAYNKIKQSSLFSTGFLSLRAHLTRTEPGDCQLDAQLTETRHLRVSIEPMSGMSILSATPTVSDRSDLERNPERSSVEDIVSRVARIRCNAAIEEIESKVKMLGFVPLNPRAWKVDIRRIFPPNVLRFTLFSHQLWGRNWIVAATSSMDGDSWWVVQLRTTVPAKGPPVPHGSGRGQSVLRSAQVVTDSFFPARYDIDDAYLANLGHSLSGILAIHSNARYLSDLQSIKFHPPPHKLMIEPDLRVPDILVRYDVSTLPSVFRAAMPVKAKKKSLIRDTIRLAFHGVDPREKCAIMVAYGNLVDSAAALGPLISSWDRSLVFQKGGSGFAMRLLAPAGHPVIASLLENLQRLECVLSVLESLQRKKVEIRTFSLSSISFVYGPERDLAASLNIHLSRNESLMELSPTELASRSESLFGLRLGIQFGLQNPHRRIQESLASSLNRSSTEAGLETVVELLTLTLPLMRALDQLMASTSYSGPLRLHVTVRNARTYQIHYPGDEARFQLVAASHQNRSVWVLKDANNQGNSKNDDSITARLRQTLYTSKGDGWKGLETGVVVEVNKVGILLRELEKCFAASRADPKHDPADSAANFNGSNKPGTSASEAEKGTADGAKFSLSDPLTTLPNDKSTLSQADTAAQKEDIIMID
Length1111
PositionTail
OrganismAspergillus eucalypticola (strain CBS 122712 / IBT 29274)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Eurotiomycetidae> Eurotiales> Aspergillaceae> Aspergillus.
Aromaticity0.07
Grand average of hydropathy-0.271
Instability index46.67
Isoelectric point9.15
Molecular weight123187.12
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	ARBA:ARBA00003669
ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP21357
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      85.75|      27|      34|     875|     908|       1
---------------------------------------------------------------------------
  835-  861 (46.73/28.01)	FSLS.SISF.VYGPERDLAASLNIHLSRN
  880-  908 (39.02/27.81)	FGLRlGIQFgLQNPHRRIQESLASSLNRS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     159.69|      52|     100|     913|     965|       2
---------------------------------------------------------------------------
  344-  402 (75.82/53.81)	GCFDLLhNLVLTnkvniLFKQATEL.ARGSWSEGLRVELLHRTlVVQYWTLKPGAKSWLE
  916-  968 (83.87/65.86)	TVVELL.TLTLP.....LMRALDQLmASTSYSGPLRLHVTVRN.ARTYQIHYPGDEARFQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.36|      13|      35|     291|     303|       3
---------------------------------------------------------------------------
  291-  303 (21.46/13.34)	ELDLSIGEENENS
  328-  340 (21.90/13.76)	ELEMTINDVLQNS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.74|      18|      35|     601|     618|       4
---------------------------------------------------------------------------
  601-  618 (35.59/24.70)	GRNWIVAATSSMDGDSWW
  638-  655 (30.14/19.76)	GRGQSVLRSAQVVTDSFF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.88|      10|     571|     114|     125|       7
---------------------------------------------------------------------------
  114-  125 (17.80/17.12)	EMQvnSHEFHPP
  688-  697 (21.08/12.45)	DLQ..SIKFHPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.16|      19|      44|     473|     492|       9
---------------------------------------------------------------------------
  473-  492 (29.07/22.43)	SSLFSTGFLSLRAHLTRtEP
  518-  536 (33.09/20.65)	SILSATPTVSDRSDLER.NP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     109.23|      36|     488|     213|     249|      10
---------------------------------------------------------------------------
  213-  249 (58.43/42.43)	NPDLKTAlEVLSRGKVASM.SDF....GYKPPRRLTVRGTLR
  704-  744 (50.80/31.93)	EPDLRVP.DILVRYDVSTLpSVFraamPVKAKKKSLIRDTIR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP21357 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AEMQVNSHEFHPPTAPTTGKSPGNQSPGNIRK
2) SRADPKHDPADSAANFNGSNKPGTSASEAEKGTADGAKFSLSDPLTTLPNDKSTLSQADTA
3) VMDNANIGGLRHGPGNTYPQDGLSHPRGEVAQFNGANAQDGPVYVNGVEKNASQTRSVETVSANHASRVTKGPPE
113
1041
5
144
1101
79










Molecular Recognition Features






MoRF SequenceStartStop




1) KEDIIMI
2) KKLRIL
1104
144
1110
149