<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP21138

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMNLKYYASPSDLLDDKTTSPIILHENTVPRCLGMNASVTIEGTSSMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFEAQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGDTKFKGNSQADTVDFSIIAVAGKALGPADIMEHHSGSQSPLLTTGDLGKEKSQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKSSSHNQYTSSGSVSSSGSKSHHSHSSSSSSSASASGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSTLMNPSLSKPNISPSHSRPPGASDKLSSPMKPIPGTPPSSKAKSPISSGSGGSHISGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDQMDGQMGVSTNSSHSMSSKHNMSGGEFQGKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHRKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1361
PositionMiddle
OrganismTrichechus manatus latirostris (Florida manatee)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Afrotheria> Sirenia> Trichechidae> Trichechus.
Aromaticity0.04
Grand average of hydropathy-0.724
Instability index53.73
Isoelectric point8.92
Molecular weight143996.31
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP21138
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             9|     375.17|      40|      41|     866|     905|       1
---------------------------------------------------------------------------
  756-  781 (35.43/ 7.06)	GNGT.......SN....................SSLSG.....PGLD..S.KPGKRSRTPS
  782-  827 (44.59/11.54)	NDGK.......SKDKPPkrkkadTEG.KSP.SHSSSNR.....PFTPptS.TGGSKSPGSS
  866-  901 (55.74/16.99)	...........SSGSVS......SSGSKSHHSHSSSSS.....SSAS..A.SGKMKSSKSE
  902-  925 (35.12/ 6.91)	GSSS.............................SKLSS.....SMYS..S.QGSSGSSQSK
  926-  956 (43.83/11.17)	NSSQ.......SGGKPG......SSPITKH......GL.....SSGS..S.STKMK...PQ
  957- 1008 (38.85/ 8.73)	GKPStlmnpslSKPNIS......PSHSRPPGASDKLSSpmkpiPGTP..P.SSKAKSPISS
 1009- 1042 (39.52/ 9.06)	GSGG.....shISGTSS......SSGMKS....SSGLG.....SSGS..L.SQK....TPP
 1043- 1081 (46.23/12.34)	SSNS....ctaSSSSFS......SSGS....SMSSSQN.....QHGS..S.KGKSPSRNKK
 1210- 1246 (35.86/ 7.27)	...........NYGSPL......ISGSTPKHERGSPSH.....SKSP..AyTPQNLDSESE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     148.89|      46|      50|    1089|    1137|       2
---------------------------------------------------------------------------
 1089- 1121 (45.07/21.34)	.................DKLKHGVVTSGPGGEDQMdGQMGVSTNSSHSMS
 1122- 1163 (57.97/30.66)	SKHNmsGGEFQGKREKsDKDKSKVSTSG........GSVDSSKKTSESKN
 1175- 1205 (45.85/18.29)	SKHD..GGSPSIKAKV.TLQKPG.ESSGEGLRPQM...............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     292.06|      40|      41|     566|     605|       3
---------------------------------------------------------------------------
  397-  439 (29.44/ 6.66)	....ST..igSS..PTP.....PHHTP.....PPVSSMAGNTKN...HPmlmN............llkDnPA.QDFS
  440-  463 (28.59/ 6.24)	TLY.GS....S...........PL........ERQNSSSGSPR.............................mEMCS
  464-  505 (39.99/11.93)	GSN.KTkkkkSS..RLP.....P.DKP.....KHQTEDD..FQRELFSM...D..............vD.SQ.NPIF
  506-  531 (29.98/ 6.93)	DVN.MT....ADtlDTP.....HI.TP.................A...P...S...............Q.CS.TPPT
  532-  572 (40.15/12.01)	TYPqPI....PH..PQP.....SIQRM.....VRLSSSDSIGPDV...T...D............ilsD.IA.EEAS
  573-  627 (57.53/20.70)	KLP.ST....SD..DCP.....PIGTP.....VRDSSSSGHSQSALFDP...DvfqtnnnenpytdpaD.LI.ADAA
  628-  681 (33.50/ 8.69)	GSP.SS........DSPtnhffPDGVDfnpdlLNSQSQSGFGEE.YFDE...S........sqsgdndD.FK.GFAS
  682-  730 (32.87/ 8.38)	QAL.NT....L...GVP.....MLGGD.....NGDTKFKGNSQADTVDF...S...iiavagkalgpaD.IM.EH..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     198.09|      49|      53|     135|     187|       4
---------------------------------------------------------------------------
   91-  134 (60.91/35.68)	PIPVS...RA..FVQKL...QNC.......TGIP..LF.EAQPTYVPLYELITqFelsKDPD
  135-  183 (93.67/62.04)	PIPLN...HNMRFYAALPGQQHCY...FLNKDAP..LP.DGRSLQGTLVSKIT.F...QHPG
  189-  238 (43.51/32.90)	...LNlirHQVA.YNTLIGS..CVkrtILKEDSPglLQfEVCPLSESRFS.VS.F...QHP.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.03|      17|      19|     322|     339|       6
---------------------------------------------------------------------------
  322-  339 (30.58/22.93)	PPASSPGYGMTTGNNpMS
  344-  360 (34.45/20.22)	PTNTFPGGPITTLFN.MS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.90|      12|      17|    1275|    1286|       7
---------------------------------------------------------------------------
 1275- 1286 (20.40/13.09)	EKHRKHKKEKKK
 1293- 1304 (20.49/13.18)	DRDRDKDRDKKK
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP21138 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AGKALGPADIMEHHSGSQSPLLTTGDLGKEKSQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKSSSHNQYTSSGSVSSSGSKSHHSHSSSSSSSASASGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSTLMNPSLSKPNISPSHSRPPGASDKLSSPMKPIPGTPPSSKAKSPISSGSGGSHISGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDQMDGQMGVSTNSSHSMSSKHNMSGGEFQGKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHRKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
2) KNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGP
3) LFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFN
4) LLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGDTKFKGNSQAD
718
319
356
650
1361
352
647
708










Molecular Recognition Features






MoRF SequenceStartStop




1) GEEYF
2) HKKEKKKVKD
3) IKAKVTL
4) ITIQI
5) PSLTAVIDKLKHG
6) RLSPDFMIGEEDDDLMD
7) VAKIIISK
660
1280
1184
844
1082
1339
1169
664
1289
1190
848
1094
1355
1176