<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP21126

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMYLALQSLEQDLSKMAIMYWKATNASPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENTVPRCLGMNASVTIEGTSSMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFEAQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGDTKFKGNSQADTVDFSIIAVAGKALGPADIMEHHSGSQSPLLTTGDLGKEKSQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKSSSHNQYTSSGSVSSSGSKSHHSHSSSSSSSASASGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSTLMNPSLSKPNISPSHSRPPGASDKLSSPMKPIPGTPPSSKAKSPISSGSGGSHISGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDQMDGQMGVSTNSSHSMSSKHNMSGGEFQGKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHRKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1408
PositionMiddle
OrganismTrichechus manatus latirostris (Florida manatee)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Afrotheria> Sirenia> Trichechidae> Trichechus.
Aromaticity0.04
Grand average of hydropathy-0.701
Instability index54.04
Isoelectric point8.94
Molecular weight149185.31
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP21126
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     349.18|      40|      41|     913|     952|       1
---------------------------------------------------------------------------
  832-  877 (45.00/10.76)	K.......SKDKPPkrkkadTEG.KSP.SHSSSNR.....PFTPptS.TG..GSKSPGSSGRS
  913-  951 (62.33/18.52)	........SSGSVS......SSGSKSHHSHSSSSS.....SSAS..A.SG..KMKSSKSEGSS
  976- 1006 (43.39/10.03)	Q.......SGGKPG......SSPITKH......GL.....SSGS..S.ST..KMK...PQGKP
 1007- 1058 (40.92/ 8.93)	StlmnpslSKPNIS......PSHSRPPGASDKLSSpmkpiPGTP..P.SS..KAKSPISSGSG
 1059- 1092 (36.15/ 6.79)	G.....shISGTSS......SSGMKS....SSGLG.....SSGS..L.SQ..K....TPPSSN
 1093- 1131 (44.69/10.62)	S....ctaSSSSFS......SSGS....SMSSSQN.....QHGS..S.KG..KSPSRNKKPSL
 1189- 1228 (38.10/ 7.66)	K.......SK..VS......TSGGSVDSSKKTSES.....KNVG..S.TGvaKIIISKHDGGS
 1257- 1296 (38.61/ 7.89)	........NYGSPL......ISGSTPKHERGSPSH.....SKSP..AyTP..QNLDSESESGS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     219.11|      49|      50|     152|     201|       2
---------------------------------------------------------------------------
  100-  150 (41.27/18.07)	....PLIMgSHPVdnkWTPSFSSITsansvdlpaCFFL.KFPQPIPVSRA...F...VQKL...Q..
  152-  201 (89.46/52.84)	CTGIPLFE.AQPT...YVPLYELIT.........QFELSKDPDPIPLNHNM.RF...YAALPGQQhC
  210-  252 (49.26/23.33)	.....LPD.GRSL...QGTLVSKIT..........F...QHPGRVPLILNLiRHqvaYNTLIGS..C
  262-  290 (39.11/16.72)	SPGLLQFE.V.......CPLSE..S.........RFSVSFQH...PVNDSL................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     364.01|      64|      64|     377|     440|       3
---------------------------------------------------------------------------
  377-  427 (81.85/36.68)	...............GMT..T..G...NN..PM.....SGTTTPTN.TFPGGPIT....TLFNM.SMSI...KDRHESVGHGED.FSK......V.S
  428-  478 (72.92/31.57)	QNPILTSLLQITGngGST..I..G...SS..P.........TPPHH.TPP..PVS....S...M.AGNT...KN......HPML.MNL......L.K
  479-  547 (51.09/19.10)	DNP........AQ..DFS..TlyG...SS..PL.erqnSSSGSPRM.EMCSGSNK....TKKKK.SSRLppdKPKHQT...EDD.FQRelfsmdVdS
  548-  609 (52.15/19.70)	QNPIFD..VNMTA...........dtlDT..PHitpapSQCSTPPT.TYP.QPIPhpqpSIQRMvRLSS...SD...SI..GPD.VTD.........
  610-  660 (43.43/14.72)	...ILSDIAEEAS..KLP..S..T...SDdcPP.....IGTPVRDS.SSSGHSQS....ALFDP.DVFQ...TNNNE....................
  661-  715 (62.57/25.66)	.NPYTDPADLI.......adA..A...GS..PS.....S..DSPTNhFFPDG.......VDFNP.DL.L...NSQSQS.GFGEEyFDE......S.S
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.90|      12|      15|    1322|    1333|       5
---------------------------------------------------------------------------
 1322- 1333 (20.40/11.20)	EKHRKHKKEKKK
 1340- 1351 (20.49/11.28)	DRDRDKDRDKKK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.64|      20|      23|     766|     785|       7
---------------------------------------------------------------------------
  766-  785 (36.25/18.36)	GKALGPADIMEHHSGSQSPL
  792-  811 (30.39/14.03)	GKEKSQKRVKEGNGTSNSSL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP21126 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AGKALGPADIMEHHSGSQSPLLTTGDLGKEKSQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKSSSHNQYTSSGSVSSSGSKSHHSHSSSSSSSASASGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSTLMNPSLSKPNISPSHSRPPGASDKLSSPMKPIPGTPPSSKAKSPISSGSGGSHISGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDQMDGQMGVSTNSSHSMSSKHNMSGGEFQGKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHRKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
2) KNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGP
3) LFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPIPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFN
4) LLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGDTKFKGNSQAD
765
366
403
697
1408
399
694
755










Molecular Recognition Features






MoRF SequenceStartStop




1) GEEYF
2) IKAKVTL
3) ITIQIP
4) KHKKEKKKVKD
5) PSLTAVIDKLKHG
6) RLSPDFMIGEEDDDLMD
7) VAKIIISK
707
1231
891
1326
1129
1386
1216
711
1237
896
1336
1141
1402
1223