<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP20754

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMLPHKNNQSTNSVPISRVGSSAKLNHLNVPGSNSRPTSRPNTPSSYVTSSLNPTRNIPVTSNSINSKIKNQEEAALFEALPITSSIRDFENELVELSSSISSFKEENIPSHVERLIQIDKTLSSEQQNLRKHQELGVEIETLSKENEDLSKESSRFLKELIACRAELKKLPRLPAAQTSSRSSRLKEIGVQELLDYSMTLAKFSKAPTTASGQMPHPNNFIWPAEDALRRGLLALTSLKSDEVIRAELGEPEAEAKEEKEKEEDDDIEMEDVQDMSAPTTKVHREEHAPRKIEVNKAPQALNLDLFDGDDSDDSD
Length315
PositionMiddle
Organism[Candida] haemulonis
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Metschnikowiaceae> Clavispora> Clavispora/Candida clade.
Aromaticity0.03
Grand average of hydropathy-0.771
Instability index58.89
Isoelectric point5.07
Molecular weight35078.59
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364141

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP20754
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.82|      19|      20|     106|     125|       1
---------------------------------------------------------------------------
  106-  125 (26.55/21.51)	ENIPSHVERLIQIDkTLSSE
  127-  145 (30.27/19.45)	QNLRKHQELGVEIE.TLSKE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      70.17|      20|      21|       2|      21|       2
---------------------------------------------------------------------------
    2-   21 (37.02/21.42)	LPHKNNQSTNSVPISR..VGSS
   24-   45 (33.15/18.51)	LNHLNVPGSNSRPTSRpnTPSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      93.59|      28|      70|     206|     233|       3
---------------------------------------------------------------------------
  206-  233 (52.87/32.09)	APTTASGQMPH.PNNF.IWPAEDALRRGLL
  277-  306 (40.72/23.27)	APTTKVHREEHaPRKIeVNKAPQALNLDLF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      62.55|      17|      20|      57|      73|       5
---------------------------------------------------------------------------
   57-   73 (28.14/18.99)	IPVTSNSINSKIKNQEE
   80-   91 (20.00/11.31)	LPITSS.....IRDFEN
   92-  105 (14.41/ 6.04)	ELVELSSSISSFKE...
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP20754 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MLPHKNNQSTNSVPISRVGSSAKLNHLNVPGSNSRPTSRPNTPSSYVTSSLNPTRNIPVTSNSIN
2) VIRAELGEPEAEAKEEKEKEEDDDIEMEDVQDMSAPTTKVHREEHAPRKIEVNKAPQALNLDLFDGDDSDDSD
1
243
65
315










Molecular Recognition Features






MoRF SequenceStartStop




1) APTTKVHREEHAPRKIEVNKAPQALNLDLFDGDDSDDSD
277
315