<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP20571

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQGGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIISVAGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPAGSDKLASPMKAVPGTPPSSKAKSPISSGSGGSHMSGTSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSLTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN
Length1410
PositionMiddle
OrganismLeptonychotes weddellii (Weddell seal) (Otaria weddellii)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Carnivora> Caniformia> Phocidae> Leptonychotes.
Aromaticity0.04
Grand average of hydropathy-0.702
Instability index54.54
Isoelectric point8.95
Molecular weight149224.32
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP20571
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     256.64|      47|      50|     793|     839|       1
---------------------------------------------------------------------------
  793-  839 (77.32/23.57)	KEKTQKRVKEGNGTSNS.SLSGPGLDSKPG.KRSRTPSNDGKSKDKPPK
  840-  882 (54.92/13.98)	RKKADT...EGKSPSHS.SSNRPFTPPTST.GGSKSPGSSGRSQ.TPPG
 1047- 1090 (46.52/10.38)	SSKAKSPISSGSGGSH...MSGTS..SSTGmKSSSGLGSSGSLSQKTPP
 1091- 1125 (40.54/ 7.82)	SSNS......CTASSSSfSSSGSSMSSSQN.QH.......GSSKGKSPS
 1132- 1174 (37.35/ 6.45)	LTAVIDKLKHGVVTSGP.GGEDP.MDGQMG.VSTNSSSHPMSSKH...N
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.16|      20|      22|     912|     933|       2
---------------------------------------------------------------------------
  913-  933 (29.79/ 8.97)	SGSVSSSgSKSHHSHSSSSSS
 1292- 1311 (27.37/ 7.30)	SESESGS.SIAEKSYQNSPSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.42|      19|      20|    1177|    1196|       4
---------------------------------------------------------------------------
 1177- 1195 (28.32/ 9.11)	GGEFQGKREK.....SDKEKSKVS
 1262- 1285 (19.10/ 6.31)	SPLISGSTPKhergsPSHSKSPAY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.26|      18|      20|     965|     982|       5
---------------------------------------------------------------------------
  965-  982 (33.18/13.58)	GSSGSSQSKNSSQSGGKP
 1010- 1027 (26.08/ 8.62)	LMNPSLSKPNISPSHSRP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      89.48|      17|      20|     369|     386|       6
---------------------------------------------------------------------------
  369-  385 (35.50/11.78)	PPASSPGYGMTTGNNPM
  391-  406 (26.04/ 6.21)	PTNTFPGGPITTLFN.M
  457-  472 (27.94/ 6.96)	PPPVSSMAG.NTKNHPM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      64.33|      13|      26|     155|     167|       7
---------------------------------------------------------------------------
  127-  141 (18.07/ 7.81)	LPacFFLKFPQPIPV
  155-  167 (24.14/12.95)	IP..LFETQPTYVPL
  183-  195 (22.11/11.23)	IP..LNHNMRFYAAL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     102.62|      23|      50|     476|     498|       8
---------------------------------------------------------------------------
  476-  498 (37.70/18.25)	LLKDNP....AQDFSTLYGSSPLERQN
  499-  519 (31.60/14.04)	SSSGSP....RMEICP..GSNKTKKKK
  523-  549 (33.32/15.23)	LPPDKPkhqtEDDFQRELFSMDVDSQN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     170.14|      32|      52|     550|     581|      10
---------------------------------------------------------------------------
  550-  571 (30.90/14.08)	......................PIFDV.NM.TADTL.D.TPHITP.......APS
  572-  590 (32.66/15.43)	QCSTPPTTYP..........................qP.VPH..P.......QPS
  596-  625 (35.84/17.87)	RLSSSDSI.G............P..DVtDI.LSDIA.E.EASKLP.......STS
  626-  676 (33.20/15.85)	DDC.PPIGTPvrdssssghsqsALFDP.DVfQTNNN.E.NPYTDPadliadaAGS
  677-  702 (37.53/19.17)	PSSDSPT..N............HFF.......PDGV.DfNPDLLN.......SQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     182.38|      48|      55|     204|     252|      13
---------------------------------------------------------------------------
  201-  249 (71.21/39.18)	.C...yfLNKDAP..LP.DGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLI
  251-  303 (60.97/34.18)	SCvkrtiLKEDSPglLQfEVCPLSESRFS.VSFQHPVNDSLV..CVVMDVQDSTHV
  304-  342 (50.20/22.79)	SC....kLYKG....LS.DALICTDDFIAKVV.QRCMSIPVTMRAIRRK.......
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.52|      12|      19|    1324|    1335|      14
---------------------------------------------------------------------------
 1324- 1335 (19.60/12.83)	EKHKKHKKEKKK
 1342- 1353 (19.91/13.16)	DRDRDKDRDKKK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.83|      13|      19|    1219|    1231|      15
---------------------------------------------------------------------------
 1219- 1231 (23.35/14.30)	AKIIISK.....HDGGSP
 1235- 1252 (16.48/ 7.56)	AKVTLQKpgessGEGLRP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.18|      13|      19|     418|     430|      16
---------------------------------------------------------------------------
  418-  430 (24.17/11.40)	GHGEDFSKVSQNP
  440-  452 (24.01/11.26)	GNGGSTIGSSPTP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP20571 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AGKALGPADLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGTSNSSLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASNSGKMKSSKSEGSSSSKLSSSVYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPAGSDKLASPMKAVPGTPPSSKAKSPISSGSGGSHMSGTSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKEKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSLTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN
2) KNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGP
3) LFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICPGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQGGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADT
765
366
403
1410
399
756










Molecular Recognition Features






MoRF SequenceStartStop




1) FGEEYF
2) IKAKVTLQ
3) IRPLPEYSTEKHKKHKKEKKKVKDK
4) KKPSLTAVIDKLKHGV
5) LSPDFMIGEEDDDLMDV
6) PKITIQIPK
7) VAKIIISK
706
1233
1315
1128
1389
889
1218
711
1240
1339
1143
1405
897
1225