<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP14107

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMLQNQAQILQSPARLGLTNPNSPSFSNPTTPKLTSQNPPHHFQQHHQQQQQTPLTTTLTTTTTSSTLLSLLPPLSRAQSLLLQMASLATKLFEVSPNRSVWLASFRGSLPTFLSSQTLTLNPPPLDSSPSSTKEILSLFTSLQTQLFESVAELQEILDLQDAKQKIAREIRTKDTAILAFANKLKEAEQVLDLLVDDYSDYRRPKRSKSEGNSDESSSSTTSIATSLKLSDVLSYAHRISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKIESKDKTIETLMEPPALPPTENNPLVDLAAVQGLIPPQLTVPSGWRPGMPVELPSDFPILPPPGWKPGDEVPLPPLGALVPGKIEDQQPRPVVRMPTKVPEPIQVRHVELDINPDSDDSSDYSSEGSSDDEDEG
Length420
PositionMiddle
OrganismMacleaya cordata
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Streptophyta> Embryophyta> Tracheophyta> Spermatophyta> Magnoliopsida> Ranunculales> Papaveraceae> Papaveroideae> Macleaya.
Aromaticity0.05
Grand average of hydropathy-0.470
Instability index68.18
Isoelectric point4.81
Molecular weight45880.06
Publications
PubMed=28552780

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP14107
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     133.93|      39|      42|     303|     344|       1
---------------------------------------------------------------------------
  303-  344 (65.74/33.15)	LPPTENNP..LVDLAAVQGLIPPQLTvPSGWRP..GMPVELPSdfP
  346-  388 (68.18/26.98)	LPPPGWKPgdEVPLPPLGALVPGKIE.DQQPRPvvRMPTKVPE..P
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      84.15|      28|      32|     104|     135|       2
---------------------------------------------------------------------------
   53-   72 (31.84/14.13)	PL...................................TTTLTT..TTTSSTLLSLLP
   73-  123 (37.57/18.11)	PL....SRAQSLllqmaslatklfevspnrsvwlaSFRGSLPT..FLSSQTLTLNPP
  124-  153 (14.75/11.65)	PLdsspSSTKEI......................lSLFTSLQTqlFESVAEL.....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.20|      20|      32|     154|     173|       3
---------------------------------------------------------------------------
  154-  173 (32.36/21.32)	QEILDLQ.DAKQKIAREIRTK
  188-  208 (29.84/19.14)	EQVLDLLvDDYSDYRRPKRSK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.55|      12|      14|      12|      23|       4
---------------------------------------------------------------------------
   12-   23 (22.89/ 8.53)	PARLGLTNPNSP
   28-   39 (23.66/ 9.03)	PTTPKLTSQNPP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP14107 with Med4 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ESKDKTIETLMEPPALPPTENNPLVDLAAVQG
2) ILQSPARLGLTNPNSPSFSNPTTPKLTSQNPPHHFQQHHQQQQQTPLTTTLT
3) PVELPSDFPILPPPGWKPGDEVPLPPLGALVPGKIEDQQPRPVVRMPTKVPEPIQVRHVELDINPDSDDSSDYSSEGSSDDEDEG
288
8
336
319
59
420










Molecular Recognition Features






MoRF SequenceStartStop




1) PRPVVRMPTKVPEPIQVRHVELDIN
375
399