<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP12529

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTSSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPIDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYVPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITLQHPGRVPIILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIISVGGKALGSADLMEHHSGSQSPLVTTGELGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQCGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFASSGSSMSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSANSSHPMSSKHNVSGGEFQSKREKSDKDKSKVSASGSSVDSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKERDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1579
PositionMiddle
OrganismTarsius syrichta (Philippine tarsier)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Tarsiiformes> Tarsiidae> Carlito.
Aromaticity0.04
Grand average of hydropathy-0.677
Instability index53.71
Isoelectric point8.87
Molecular weight168267.05
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:Ensembl
nucleolus	GO:0005730	IEA:Ensembl
nucleoplasm	GO:0005654	IEA:Ensembl

GO - Biological Function
chromatin binding	GO:0003682	IEA:Ensembl
estrogen receptor binding	GO:0030331	IEA:Ensembl
LBD domain binding	GO:0050693	IEA:Ensembl
nuclear receptor coactivator activity	GO:0030374	IEA:Ensembl
peroxisome proliferator activated receptor binding	GO:0042975	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IEA:Ensembl
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
vitamin D receptor binding	GO:0042809	IEA:Ensembl

GO - Biological Process
androgen biosynthetic process	GO:0006702	IEA:Ensembl
cell morphogenesis	GO:0000902	IEA:Ensembl
cellular response to epidermal growth factor stimulus	GO:0071364	IEA:Ensembl
cellular response to thyroid hormone stimulus	GO:0097067	IEA:Ensembl
fat cell differentiation	GO:0045444	IEA:Ensembl
intracellular steroid hormone receptor signaling pathway	GO:0030518	IEA:Ensembl
keratinocyte differentiation	GO:0030216	IEA:Ensembl
negative regulation of keratinocyte proliferation	GO:0010839	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IEA:Ensembl
positive regulation of gene expression	GO:0010628	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	IEA:Ensembl
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP12529
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.86|      20|      21|    1088|    1108|       1
---------------------------------------------------------------------------
 1084- 1103 (36.16/10.19)	TSSGSVSSSGS.KSHHSHSSS
 1105- 1125 (24.70/ 6.70)	SSSSSASTSGKmKSSKSEGSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     503.16|      65|      65|    1357|    1421|       2
---------------------------------------------------------------------------
  930- 1017 (50.13/13.04)	DFSIISVGGKA...LGSadlmehHSGS.QSPLVttGELGKEKT..QKrvK..EGNG........TS...N.........STLS......GPG.LDSKPGK...........RS...............rtpsndgkskdkppkrkkADT
 1018- 1079 (65.77/20.17)	EGKSPSHSSSNrpfTPP......TSTG.GSKSP..GSSGRSQ...TP..P..GVAT........PPI.PK.........ITIQ......IPK.GTVMVG............KP................................sS..
 1134- 1205 (67.57/20.99)	....YSSQGSS...GSS......QSKN.SSQCG..GKPGSSPI..TK..HglSSGS........SSTKMKpqgkpsslmNPLS......KPN.ISPSHS............RP..............................pggSDK
 1206- 1265 (60.98/17.99)	LASPM....KP...VPG......TPPS.SK..........AKSPISS..G..SGGShmsgtsssSGMKSS.........SGLG......SSG..SLSQKT...........PP.................................SSN
 1266- 1359 (43.74/10.13)	S...CTASSSS...FAS......SGSSmSSQN....QHGSSK...........GKS........PSRNKK.........PSLTavidklKHGvVTSGPGG...........EDpmdgqmgvsansshpmsskhnvsggefqskrekSDK
 1360- 1427 (98.24/34.98)	DKSKVSASGSS...VDS......KKTS.ESKNV..GSTGVAKIIISK..H..DGGS........PSIKAK.........VTLQ......KPG.ESSGEGL...........RP..............................qmaSSK
 1428- 1500 (75.05/24.40)	NYGSPLISGST...PKH......ERGS.PSHSK..SPAYTPQNLDSE..S..ESGS.........SIAEK.........SYQN......SP...SSDDGI...........RP......................lpeystekhkkHKK
 1501- 1555 (41.68/ 9.18)	EKKKVKDKDRD...RDR......DKER.DKKK....S...........................HSIKPE..........SWS......KSP.ISSDQSLsmtsntilsadRP....................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     384.82|      64|      64|     549|     612|       3
---------------------------------------------------------------------------
  549-  600 (89.02/32.47)	................GM..T..TGNNPM.....SGTTT...PTN.TFP...GGPI......TTLFNM.SMS.IKDRHESVGHGED..F.S.KV.SQ
  601-  662 (72.23/24.97)	NPILTSLLQITG..ngGS..T..IGSSP.........TP...PHH.TPP.....PVssmagnTKNHPM.LMNlLKD.....NPAQD..F.S.TL.YG
  663-  720 (55.89/17.67)	SSPLERQNSSSGsprmEM..C..SGSNKT.....KKKKSsrlP.....P...DKP...................KHQTEDDFQREL..F.SmDVdSQ
  721-  781 (53.86/16.76)	NPIFD..........vNM..TadTLDTPHitpapSQCST...PPT.TYPqpvPHPQ......PSIQRMvRLS.SSD...SI..GPD..V.T.D....
  782-  827 (43.27/12.03)	..ILSDIAEEAS....KLpsT..SDDCP..........................PI......GTPVRD.S........SSSGHSQStlFdS.DV.FQ
  833-  888 (70.55/24.22)	NPYTDPADLIA...........dAAGSPS.....S..DS...PTNhFFP...DG.........VDFNP.DL..LNSQSQS.GFGEE.yF.D.ES.SQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.31|      12|      22|     890|     902|       4
---------------------------------------------------------------------------
  890-  902 (20.18/16.48)	GDNDD..FKGfASQA
  913-  926 (20.13/10.38)	GDNGEtkFKG.NSQA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     209.57|      48|      48|     152|     199|       5
---------------------------------------------------------------------------
  152-  182 (39.58/18.65)	.........................KHL..KGLV.NLYNL..........P..GDNKLKTK.MYLALQSLEQ
  183-  233 (68.01/37.51)	DLSKMAIMYWKATNAGP......ldKIL..HGSV.G.YLT..........PrsGGHLMNLK.YYASPSDLLD
  234-  296 (53.10/27.62)	DKTSSPIILHE..NNVPrslgmnasVTI..EGTS.AMYKLpiaplimgshP..IDNKW.TP.SFSSITSANS
  298-  342 (48.88/24.82)	DLP..ACFFLKFPQPIP...........vsRAFVqKLQNC..........T..GIPLFETQpTYVPLYEL..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.30|      18|      22|     447|     464|       6
---------------------------------------------------------------------------
  447-  464 (32.49/18.52)	ES.RFSVSFQHPVNDSLVC
  471-  489 (27.81/14.80)	DStHVSCKLYKGLSDALIC
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP12529 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GGKALGSADLMEHHSGSQSPLVTTGELGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQCGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFASSGSSMSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSANSSHPMSSKHNVSGGEFQSKREKSDKDKSKVSASGSSVDSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKERDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
2) KNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGP
3) LFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFN
4) LLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTV
937
538
575
869
1579
571
866
929










Molecular Recognition Features






MoRF SequenceStartStop




1) FGEEYFDE
2) IRPLPEYSTEKHKKHKKEKKKVKDK
3) KITIQIPK
4) KKPSLTAVIDKLKHGV
5) RLSPDFMIGEEDDDLMDVALI
6) SIKAKVTLQK
7) VAKIIISKH
878
1484
1062
1299
1557
1401
1387
885
1508
1069
1314
1577
1410
1395