<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP12482

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESERLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNIKYYASPSDLLDDKTASPIILHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSVTSANSVDLPACFFLKFPKPIPVSKTFVQKLQNCTGIPLFETPPTYVPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTVLKEDTPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMATGNNPMSGTTTPTNTFPGGPITTLFNMSMSIRDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRVPPDKSKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQTVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKSSGQADTVDFSIISVAGKALGPADIMEHHSGSQSPLMATGELGKEKTQKRVKEGNGTGGGSGSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSHSQYSSSGSVSSSGSKSHHSHSSSSSSSSSASTSGKMKSSKSEGSSSSKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPCLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSSGPHMSGTSSSSGIKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDSQMGVSTNSSSHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTLSMTNNPILSADRPSRLSPDFMMGEEDDDLMDVALIGN
Length1580
PositionMiddle
OrganismMesocricetus auratus (Golden hamster)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Glires> Rodentia> Myomorpha> Muroidea> Cricetidae> Cricetinae> Mesocricetus.
Aromaticity0.04
Grand average of hydropathy-0.681
Instability index53.29
Isoelectric point8.90
Molecular weight168190.00
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP12482
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     317.41|      42|      42|    1083|    1124|       1
---------------------------------------------------------------------------
 1024- 1098 (46.85/10.71)	HSSSNRPFTPPTST.....G..........GSK.....SPGSSgrsqtppgvatppipkitiqipkgtvmvgkpshsqyS...SSGSVSSSGSKSHHS
 1099- 1145 (50.45/12.21)	HSSSSSSSSSASTS.....GKMK.......SSK.....SEGSS...........................ssklsgsmyS...SQGSSGSSQSK....
 1194- 1247 (39.29/ 7.57)	PSHSRPPGGSDKLA.....SPMKpvpgtppSSKakspiSSGSS.....................................gphMSGTSSSSGIKS..S
 1248- 1289 (54.50/13.90)	SGSGSSGSLSQKTP.....PASN.......SCT.....PSSSS...................................fS...SSGS.SMSSSQNQHG
 1290- 1340 (36.41/ 6.37)	SSKGKSPSRNKKPSltaviDKLK.hgvvtsGPG.....GEDPM....................................D...SQMGVSTNSSS..HP
 1398- 1445 (46.90/10.73)	HDGGSPSIKAKVTL.....QKPG.......ESS.....GEGLR..............................pqiassK...NYGSPLISGSTPKHE
 1446- 1497 (43.02/ 9.12)	RGSPSHSKSPAYTP.....QNVD.......SES.....ESGSS..........................iaeksyqnspS...SDDGIRPLPEYSTEK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     227.06|      53|      53|     696|     748|       2
---------------------------------------------------------------------------
  593-  658 (46.41/17.38)	EDF.S.KV.....SQNPILTslLQITGNGGSTigssptpPHHTPPPvSSMAGN........tknH.PmlmNLLKDNPAQDFS.
  659-  710 (51.96/20.57)	TLYgSSPL....ERQNSS.S..GS...................PRM.EMCSGSnktkkkkssrvP.P...DKSKHQTEDDFQR
  711-  765 (72.06/32.12)	ELF.SMDV....DSQNPMFD..VSMTADALDT.......PHITPAP.SQCSTP...pttypqtvPhP...QPS.......IQR
  819-  880 (56.63/23.25)	ALF.DSDVfqtnNNENPYTD.pADLIADAAGS.......PS.SDSP.TNHFFP......dgvdfN.P...DLLNSQSQSGFGE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     193.62|      49|      53|     354|     406|       3
---------------------------------------------------------------------------
  311-  353 (53.89/27.67)	.IP..VS...KT..FVQKL...QNCT.......GIP..LF.ETPPTYVPLYELITqFelsKDPD
  354-  402 (92.93/48.37)	PLP..LN...HNMRFYAALPGQQHCY...FLNKDAP..LP.DGQSLQGTLVSKIT.F...QHPG
  405-  457 (46.80/25.64)	P..liLNmirHQVA.YNTLIGS..CVkrtVLKEDTPglLQfEVCPLSESRFS.VS.F...QHP.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     130.78|      30|     156|     991|    1023|       4
---------------------------------------------------------------------------
  952-  976 (34.83/10.80)	GSQSPLMATGELGKEKTQK.....RVK...EGN...
  993- 1023 (50.82/32.18)	GKRSRTPSNDGKSKDKPPK.....RKKADTEGKsPS
 1047- 1078 (45.12/18.13)	G.RSQTP..PGVATPPIPKitiqiPKGTVMVGK.PS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.46|      18|      20|    1343|    1360|       6
---------------------------------------------------------------------------
 1343- 1360 (32.34/15.35)	SKHNMSGGEFQSKREKSD
 1364- 1381 (29.12/13.05)	SKVSTSGGSVDSSKKTSE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      63.63|      17|      22|     541|     558|       7
---------------------------------------------------------------------------
  541-  558 (29.75/19.81)	PPASSPGYGMATGNNpMS
  563-  579 (33.88/17.57)	PTNTFPGGPITTLFN.MS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     129.85|      44|      65|     153|     197|       8
---------------------------------------------------------------------------
  165-  213 (67.87/47.59)	GDNKLKTKMYLALQSLEQDLSKMAIMYWKATNA....tpldkILHGSVG.YLTP
  216-  269 (61.98/48.07)	GGHLMNIKYYASPSDLLDDKTASPIILHEKNVPrslgmnasvTIEGTSAmYKLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      68.66|      19|      22|    1147|    1165|       9
---------------------------------------------------------------------------
 1147- 1165 (37.50/15.74)	SSQTGGKPGSSP..ITKHGLS
 1168- 1188 (31.15/11.69)	SSSTKMKPQGKPssLMNPCLS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.24|      19|     405|      48|      69|      10
---------------------------------------------------------------------------
   48-   69 (26.62/22.42)	VVMSSGGHQHlVSCleTLQKAL
  465-  483 (35.62/18.92)	VVMDVQDSTH.VSC..KLYKGL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      33.71|      10|      54|    1472|    1481|      14
---------------------------------------------------------------------------
 1472- 1481 (16.78/ 7.02)	IAEKSYQNSP
 1527- 1536 (16.94/ 7.17)	IKPENWSKSP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP12482 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FSIISVAGKALGPADIMEHHSGSQSPLMATGELGKEKTQKRVKEGNGTGGGSGSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSHSQYSSSGSVSSSGSKSHHSHSSSSSSSSSASTSGKMKSSKSEGSSSSKLSGSMYSSQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPCLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSSGPHMSGTSSSSGIKSSSGSGSSGSLSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDSQMGVSTNSSSHPMSSKHNMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTLSMTNNPILSADRPSRLSPDFMMGEEDDDLMDVALIGN
2) KNLPPASSPGYGMATGNNPMSGTTTPTNTFPGGPITTLFNMSMSIRDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRVPPDKSKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQTVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTNDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFN
3) LLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKSSGQADT
931
538
869
1580
866
928










Molecular Recognition Features






MoRF SequenceStartStop




1) FGEEYFD
2) IKAKVTLQ
3) IPKITIQIPK
4) IRPLPEYSTEKHKKHKKEKKKVK
5) KPSLTAVIDKLKHGV
6) LRPQIA
7) RLSPDFMMGEEDDDLMDVALI
8) VAKIIISKH
878
1405
1060
1487
1301
1422
1558
1390
884
1412
1069
1509
1315
1427
1578
1398