<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP12218

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAELGQQTVDFSALVSRTAEESYATLNELVEKCKSSDLSDSEKKIGILKYIVKTQQRMLRLNVLSKWCQQGTDGGRKISQDGESDSASLRTPGLKILYWLDLDKNSSTSEIGTCPFIKIEPGLDLRIKCLHSTFAIDPLTGKEAEFSLDQSCIDVEKLLLRVICCNRYTRLLEIYKELEKNGQICRVPGDVQLQGHVEEMLADSRKKDNKFDSREYQGQEVLRVRAFGSSFFTLAINIRNGHFILHSSKNVISSLVVVECEEALNQRSMSAADAFISLRSKSILHLFACIGRFLGLEVFEHGSAAVKVPKSISCGTNLLLMGFPECGSSYLLLMELDKDFKPVFKLLESQSDTPEKAQSLADLSNVVRVKTVDVGRMQICEDELNLSLLNSKKLLSILPSDGGSHQTSESSLLADFSLERSLVSSSAPSTFCSIVDEIFELEKGSSVPSFSGQIPPSTFGASPASHLGSGVANYQCLKVGTLSPKWDRGTGNYSSSMYKGVIQSGSAGSLATAPGRSQTVKKLTASKSEQDLTSLRSPHSAGAGSYTSLDEDQLTVSTNRPARLLSPPQRACPPASALSGKASAPRNSAVGTVPVGFRTSESNSLVLSPGSQAIDSATCTQSEQDAASRYNILPRKRTLSDLLDSLPSLQAMESNEGSHKRRKLVESGTHLPKSLMLISSDISGKAEEYSYGSLIAEANKGNAPSSIYVSSLLHVVRHCSLCIKHARLTSQMEALEIPYVEEVGLRSASSNLWFRVPFARDDAWQHICLRLGRPGSMYWDVKINDQHFQDLWELQKGSNSTPWGSGVRIANTSDADSHIRYDSEGVVLSYYSVNADSIKKLVADIQRLSNARTFALGMRKLLGARADEKFEDSNANSENKAPAAIKGASDATDRISEQMRKQFRIEAVGLMSLWFSFGSGVLARFVVEWESGKEGCTMHVSPDQLWPHTKFLEDFINGAEVASLLDCIRLTAGPLHALAAATRPARAAPVSGVPGVTAPMSSVAKQTSYVPSLPSNVNTSINQPAPGPGVNPVSATVGALGMHHPNAAMLAAAAAAAGRGGPGIVPSSLLPIDVSVVLRGPYWIRIIYRKKFAVDMRCFAGDQVWLQPATPPKGGPEVGGSLPCPQFRPFIMEHVAQELNGIDSNFTGSQQVVGLTNSNSLNAGSQLPAANTNRTNLSNSTGLVRPANAVTAFNRTANGLPAASNLAVVNAGMPLRRPPGTGVPAHVRGELNTAIIGLGDDGGYGGGWVPLVALKKVLRGILKYLGVLWLFAQLPDLLKEILGSILKDNEGALLNLDQEQPALRFFVGGYVFAVSVHRVQLLLQVISVKRFHQSQQQQQQNPGSAQEELTQAEIGEICDYFSRRVASEPYDASRVASFITLLTLPISVLREFLKLIAWKKGLSQVQGGDMVPTQKSRIELCLENHAGCSIDGSSENTSASKSNIHYDRAHNSVDFALTVVLDHAHIPHINAAGGAAWLPYCVSVRLRYAFGENPNVLFLGMEGSHGGRACWLRVDDWERCKQRVARTVEVNGNSAGDANQGRLRVVADSVQRTLHAYLQGLRDGGGVAAGIGL
Length1573
PositionTail
OrganismNicotiana tabacum (Common tobacco)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Streptophyta> Embryophyta> Tracheophyta> Spermatophyta> Magnoliopsida> eudicotyledons> Gunneridae> Pentapetalae> asterids> lamiids> Solanales> Solanaceae> Nicotianoideae> Nicotianeae> Nicotiana.
Aromaticity0.06
Grand average of hydropathy-0.164
Instability index46.76
Isoelectric point8.07
Molecular weight169920.08
Publications
PubMed=24807620

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IBA:GO_Central
GO - Biological Function
transcription coregulator activity	GO:0003712	IBA:GO_Central
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP12218
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.35|      16|      22|     411|     429|       2
---------------------------------------------------------------------------
  411-  429 (22.43/18.76)	SLLAD.FSLERslvSSSAPS
  433-  449 (23.91/11.96)	SIVDEiFELEK...GSSVPS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.19|      12|      16|     684|     695|       3
---------------------------------------------------------------------------
  684-  695 (21.44/13.51)	GKAEEYSY.GSLI
  701-  713 (16.75/ 8.65)	GNAPSSIYvSSLL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.31|      19|      22|    1248|    1268|       4
---------------------------------------------------------------------------
 1244- 1263 (33.23/24.23)	Y.GGGWV....PLVaLKKVLRGILK
 1264- 1287 (26.09/18.19)	YlGVLWLfaqlPDL.LKEILGSILK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     249.93|      71|     160|     723|     796|       5
---------------------------------------------------------------------------
  595-  625 (35.77/17.91)	.........................VGFRTSESN..................SLVLS...PG.SQAIDSATCTQSEQD......
  723-  796 (128.61/98.77)	IKHA.....RLTSQMEalEIPYVEEVGLRSASSNLWFRVP...FAR.DDAWQHICLRlGRPG.SMYWDVKINDQHFQDLWELQK
  885-  950 (85.56/56.00)	IKGAsdatdRISEQMR..KQFRIEAVGLMS....LWFSFGsgvLARfVVEWES.....GKEGcTMHVS...PDQ....LWPHTK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      71.23|      21|      21|      81|     101|       6
---------------------------------------------------------------------------
   81-  101 (38.20/22.63)	DGESDSASLRT.PGLKILYWLD
  103-  124 (33.03/18.57)	DKNSSTSEIGTcPFIKIEPGLD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.48|      12|      18|     806|     817|       8
---------------------------------------------------------------------------
  806-  817 (21.07/12.10)	GVRIANTS.DADS
  825-  837 (17.41/ 8.71)	GVVLSYYSvNADS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      60.70|      15|      22|    1188|    1202|       9
---------------------------------------------------------------------------
  975-  994 (17.37/ 6.96)	LHALAAATRPAraapvSGVP
 1015- 1028 (24.12/12.91)	SNVNTSINQPA.....PG.P
 1188- 1201 (19.20/ 8.57)	.NAVTAFNRTA.....NGLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.99|      14|      22|     289|     303|      10
---------------------------------------------------------------------------
  289-  303 (24.15/19.61)	CiGRFLGLEVF.EHGS
  314-  328 (24.84/14.74)	C.GTNLLLMGFpECGS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     102.88|      33|     315|     132|     208|      11
---------------------------------------------------------------------------
  153-  196 (44.51/67.65)	IDVEKLllrVICCNRYTRLLEIYKelekngQICrvPGDVQLQGH
  549-  581 (58.37/20.52)	LDEDQL...TVSTNRPARLLSPPQ......RAC..PPASALSGK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      63.22|      18|     139|     375|     405|      13
---------------------------------------------------------------------------
  388-  405 (31.25/35.87)	LLNSKKLLSILPSDGGSH
  642-  659 (31.97/ 9.93)	LLDSLPSLQAMESNEGSH
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP12218 with Med14 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AGSLATAPGRSQTVKKLTASKSEQDLTSLRSPHSAGAGSYTSLDEDQLTVSTNRPARLLSPPQRACPPASALSGKASAPRNSAVGTVPV
507
595










Molecular Recognition Features






MoRF SequenceStartStop




1) LKILY
94
98