<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP10868

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMENKKWKHELLMENLRAKSSRSLMEIAKSTRLNLLERRFNIDAVEKQTLQNALDNLQNNIQVKNRAHLIERLESITRRSLLKFTDNANTNSCFISSDMFYLEILLDPQSGKVNDVKVHHECSNESQSEPHLIDVLRKGDFIDFTQQLEGFQSIYQLNAESKIKSKAYIAIQALENDLLNIFGLECMQMQPESMVLNSSLGLAMKRQGGHPLKLLFFIRPTELLNLEMKRMDSLTDALQTANFAKRIIGNSVSINLEAAAPSNKLQITSLLIKNVGKSDAHYNFNPINLHNSTMLPATFVLRLNQAMPVSTEFIDEIKKITNNLGVFGDRSSVKLENSDDERHAPGLLISMIVNSESQGLSNNAQKGLFVTLADQSHCYFISDNPEMTGTIVKSIQFTEPSHVIKIVKMLRQQAFFNALIASCVRKPAECPNLDCFIFEVNVVSMSFIQVIVEHPIEETIVTVELDISDIRRINVKINGSEHQFDSKLENYILRVFQKTMSIPMILRSLIKYWENKSQDFQRLQKKVFNNEFYDSTSDPKRGDTNEKKDERERNSSSGTIDASYNGNGSGSGSVNNVSGAFDICGTNKNEIFFKTANDQSKQVRHDLEVNIDIFDQQRTSKMSRGMAFDLNDENDDLIRCNLMTEDMLIDNSPSPMSSNSSGDVEKNLIFQAKNRMPSQKSLDVFEFNDPSPPPAVTVIPPLQSPLAEERSKKVPTPRASPSTISTHSRVHDIEIIPLKSQSPVTEPSMLSHGSISIHPINNFYEKPKSEKKKKRKREDGDMGSPAAVKKKLSDSLGSSMANKSSGSGGQLLGKPSASFKPKKSPARGLEGIDDSTFMNFGAEQTITTVTSPLPSPQSLLKNSSSAQVNRKSSLSAVINKLKSEMSDAIATSHGDEKKGEYQIKSSGSDGIKITFNKTKSSKSPKSPKHTGLKPGVNSGPASKKSSQSHKSSSQKLLYQKSNSSGSLNPQTFTMSPKSSSQPSRSSSKERSKEGSVSPMSGFAGTSNDMLKNMLKMASTGPRPDVIKSFDKNFQIPKLSSRGKSDEKTSFEDNFKLMHPRSAPTTPVHHLTPSPTLDFMRDGGAGGNLFQPSMQNSFLTSNQQLNNKSVNQNKKLFKSVSSEHLPGEENEKFPKSSDSNDNDFQTFLRAQSKMNDQAVSMQIFKSIDLGGNMTQSGGNLQMNDSMFNDDDLSFIRNEM
Length1197
PositionMiddle
OrganismClunio marinus
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Nematocera> Chironomoidea> Chironomidae> Clunio.
Aromaticity0.06
Grand average of hydropathy-0.604
Instability index54.30
Isoelectric point8.93
Molecular weight133095.95
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP10868
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      74.72|      20|      20|    1017|    1036|       1
---------------------------------------------------------------------------
 1017- 1034 (25.52/10.01)	....STGPRPDVIKSFDKNFQI
 1035- 1055 (25.08/ 9.71)	PKlsSRG.KSDEKTSFEDNFKL
 1095- 1114 (24.12/ 9.07)	SF..LTSNQQLNNKSVNQNKKL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      71.66|      19|      20|     909|     927|       2
---------------------------------------------------------------------------
  909-  926 (27.37/13.52)	...GIKITFNKTKSS.KSPKSP
  927-  948 (21.79/ 8.98)	KhtGLKPGVNSGPASkKSSQSH
  949-  968 (22.50/ 9.57)	KssSQKLLYQKSNSS..GSLNP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.04|      22|     143|     462|     489|       4
---------------------------------------------------------------------------
  313-  336 (32.36/13.50)	IDEIKKItnNLGVFGDRSSV..KLEN
  466-  489 (33.68/21.22)	ISDIRRI..NVKINGSEHQFdsKLEN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     311.41|      84|     152|     669|     753|       5
---------------------------------------------------------------------------
  669-  753 (138.53/85.15)	FQAKNRMPSQ..KSLDVFEF.NDPSPPPAV..TVIPPLQSP..LAEERSKKVPTPRASPSTI..STHSRVHDiEIIPLKSQSPVTEPSMLSHGS
  762-  816 (60.81/30.82)	FYEKPKSEKK..KKRK.RED.GDMGSPAAVkkKLSDSLGSS..MANKSSGSGGQLLGKPSA.................................
  818-  907 (112.07/64.17)	FKPK.KSPARglEGIDDSTFmNFGAEQTIT..TVTSPLPSPqsLLKNSSSAQVNRKSSLSAVinKLKSEMSD.AIATSHGDEKKGEYQIKSSGS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     117.10|      40|      44|       2|      43|       6
---------------------------------------------------------------------------
    2-   43 (61.76/56.15)	ENKKWKHEL..LMENLRAKSSRSLME.IAKSTRLNLLerRFNIDA
   45-   87 (55.33/42.50)	EKQTLQNALdnLQNNIQVKNRAHLIErLESITRRSLL..KFTDNA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.29|      19|     156|     192|     215|      10
---------------------------------------------------------------------------
  192-  215 (29.37/29.15)	SMVLNS.SLGLAMKRQGGhplklLF
  349-  368 (30.92/17.53)	SMIVNSeSQGLSNNAQKG.....LF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     104.95|      33|     429|     534|     591|      11
---------------------------------------------------------------------------
  554-  591 (46.29/57.87)	SSSGTiDASYNGNGSGSGSVNNVSGafdICGTNkNEIF
  977- 1009 (58.66/21.26)	SSSQP.SRSSSKERSKEGSVSPMSG...FAGTS.NDML
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.45|      20|      31|    1133|    1154|      12
---------------------------------------------------------------------------
 1133- 1154 (31.94/23.44)	KSSDSNDNDFQTflRAQSKMND
 1163- 1182 (35.51/19.57)	KSIDLGGNMTQS..GGNLQMND
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      71.67|      21|     429|      99|     120|      13
---------------------------------------------------------------------------
   99-  120 (33.61/24.17)	FYlEILLDPQSGKVNDVKVHHE
  531-  551 (38.06/23.00)	FY.DSTSDPKRGDTNEKKDERE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP10868 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DSTSDPKRGDTNEKKDERERNSSSGTIDASYNGNGS
2) EQTITTVTSPLPSPQSLLKNSSSAQVNRKSSLSAVINKLKSEMSDAIATSHGDEKKGEYQIKSSGSDGIKITFNKTKSSKSPKSPKHTGLKPGVNSGPASKKSSQSHKSSSQKLLYQKSNSSGSLNPQTFTMSPKSSSQPSRSSSKERSKEGSVSPMSGFAGTSNDMLKNMLKMASTGPRPDVIKSFDKNFQIPKLSSRGKSDEKTSFEDNFKLMHPRSAPTTPVHHLTPSPTLDFMRDGGAGGNLFQPSMQNSFLTSNQQLNNKSVNQNKKLFKSVSSEHLPGEENEKFPKSSDSNDNDFQTFLRAQS
3) ISIHPINNFYEKPKSEKKKKRKREDGDMGSPAAVKKKLSDSLGSSMANKSSGSGGQLLGKPSASFKPKKSPARGLEGIDDSTF
4) KSLDVFEFNDPSPPPAVTVIPPLQSPLAEERSKKVPTPRASPSTISTHSRVHDIEIIPLKSQSPVTEPSMLSHG
5) NLMTEDMLIDNSPSPMSSNSSGDVEKNLIFQAK
533
842
754
679
640
568
1150
836
752
672










Molecular Recognition Features






MoRF SequenceStartStop




1) DLSFIRNEM
2) IKSFDKNFQI
1189
1025
1197
1034