<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP10200

Description DNA-directed RNA polymerase II holoenzyme and kornberg s mediator
SequenceMSDRLTQLQVCLNQLIEQFNSTVNYVNVHSEPALLDEDPMSVTNMAVNAPNPGQQAPPPGQAQQLNSPGERPPDSNSVSNHASVREEAEANFENTINELSTDIILKSRQISMLIDSLPGIGTSPDTQLVTINELIKELDEVERKRIEKIEEKDKLLAWCETLIKEVASGISETRS
Length175
PositionMiddle
OrganismHyphopichia burtonii NRRL Y-1933
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Debaryomycetaceae> Hyphopichia.
Aromaticity0.02
Grand average of hydropathy-0.512
Instability index48.43
Isoelectric point4.46
Molecular weight19348.40
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU366036

GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
DNA-directed 5'-3' RNA polymerase activity	GO:0003899	IEA:UniProtKB-KW
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
transcription coactivator activity	GO:0003713	IEA:EnsemblFungi
transcription corepressor activity	GO:0003714	IEA:EnsemblFungi

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP10200
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.12|      11|      18|     110|     120|       2
---------------------------------------------------------------------------
  110-  120 (18.80/12.93)	ISMLIDSLPGI
  131-  141 (17.32/11.44)	INELIKELDEV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.86|      15|      33|      32|      46|       3
---------------------------------------------------------------------------
   32-   46 (27.20/16.44)	PALLDEDPMSVTNMA
   68-   82 (28.66/17.65)	PGERPPDSNSVSNHA
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP10200 with Med21 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) NVHSEPALLDEDPMSVTNMAVNAPNPGQQAPPPGQAQQLNSPGERPPDSNSVSNHASVREEAEANF
27
92










Molecular Recognition Features






MoRF SequenceStartStop




NANANA