<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP09508

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMRLDFEIWKVGIGSENPRLQIQFSNHKLWSKITVVSNLELLQDMDMMGMQPKASPAGQTPMNSNNAPGGMHPSISNNSSMMDTSSSGVPPQQQQQGGNQMGEQNSMPLSQMLASAVNNMGGLSTTKSINTTVTPSWRLELMMEKLRQKRNQLKPLQETAKNLRQALMERRCILDPSDKLHLQKCLDTLQQSIKVNSLSSMVERLDMVSRQLGLQFTPGETGQEWFISSDMFYLEVLIDSNGVVRDIKIQHEGKTEQLASSIKSFLFITARSVLVEYQCPDLIKSLEVQDFKDFTAHLQGLISIYNLNADKIIKSKAFIALQALEADLAAMASTYSNQETGERLIMKSLLGHFQPRRGGHPVKLTYYISPYEFFDKDTKTCVPNDIAEIVKRQIGTSATVSIQGSPGINRKLQMSATVNIMRTPDGKNLPRYGTLNATNSTALPACFVINLGKPIPIFKELLTAIQTTTGIENVVTGESQPILNLVTQVVSGNQLDCSEEKGLFVTLPDQHHCYFMTETAGMEGVLISSISFTLASQLPQVIVTLRQQLTFNTLVGSCVRLCSKPVESGSIVFEVSALSHHNLSVSFQHPLDDSLVTLEFDLRDVSHPQCRVHALYSTTPDMIPTLEEHACRVIQRSMSIPVTVRAVIRRWQSMKLKTDIPKPDSLIPAPSNSSSSTNPLLPVGAPSCGLESQQTQQPTFKQCMNYNAPAQTAPIDFISYMSNPIFASLSQNFQSQSQSKNGQGQMTPHSLAASSSVLQGLLDQNNPAKRRKRNKTSTEIQKSPVRQKSPIRQEEPGAAGTAWMTELGLGLEQDGRWNIDITPVNSAAEELKRKFPVKRSVSLDSNVESEDRLPSLSITPVNATAGGTSSTVNSLLASIRPGIEIIPLPNPPIAPTIPNSITVTPILPNSTDIKKIKKRPEDMLEKKKIKKLDIKHVNKLQQQGGKPSVSTMKSVSTLPKTKKLTKSPMDMKKSGDPNKMEKDKSLVIYQPPKTQAATQAVAAALAAAKTQAVPKKSSLDSIINKLKSAAPADIGDSPGKKSEQMSTKDLLKASSSITDIIQKTASKVNEYSVKSSSGLVPGGGLKLTINKSLTAPKFGMELNMKKKTVLKRPLGQKPKSLTPLKDDLKRPLLKKEEMSKITDTRKLYMPSLEGALFKDNPPKFDLKNFQIPKKSKPIEKVETPPPAQSEKNSDLVVESIITTPSTPPQITPPRQPTPQPMVSATPTPTTSSSNPNPLPALTPIGPPPPPIITPSSVPVEPTNNVPRTEEPKIESPVVDIPPPPPPQPLPMAMPQKETIISPESPEPMDTSESIATPQNIPTTPPTTADDAPAAPTEKTSEAETVIPPRSTTPAPPPRSVTPAPPRATTPSPMTATTTTTTNNSVTSAPATVEKVAVEETSTVPPPPPPPASEPSPVVSMTCITVKSPAPNSPMLALRSPLIVQSPHTPFTIDDDLMDEALIGGGPSNNDKAASS
Length1474
PositionMiddle
OrganismOrchesella cincta (Springtail)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Collembola> Entomobryomorpha> Entomobryoidea> Orchesellidae> Orchesellinae> Orchesella.
Aromaticity0.04
Grand average of hydropathy-0.404
Instability index56.13
Isoelectric point9.08
Molecular weight159931.82
Publications
PubMed=27289101

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364059
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP09508
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     268.54|      42|      42|    1170|    1211|       1
---------------------------------------------------------------------------
  856-  908 (39.27/10.52)	SITP..VnataGGTSS...TVNSLlasirpgieiiP.LPNPPIAPTIP......NSIT....VTPILPN
 1170- 1207 (60.86/20.62)	......I....PKKSK...PIEKV...........E.TPPPAQSEKNSDLV..VESII....TTPSTPP
 1208- 1249 (51.48/16.23)	QITP.......PRQPT...PQPMV...........SaTPTPTTSSSNPNPLpaLTPI......GPPPPP
 1250- 1285 (40.64/11.16)	IITP............ssvPVEPT...........N.NVPRTEEPKIESPV..VD.......IPPPPPP
 1286- 1324 (45.56/13.46)	QPLPmaM....PQK.E...TIISP...........E.SPEPMDTS.ESIAT..PQNI.......PTTPP
 1325- 1356 (30.75/ 6.54)	T.....................TA...........D.DAPAAPTEKTSE....AETVIpprsTTPAPPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.95|      15|      15|     391|     405|       2
---------------------------------------------------------------------------
  391-  405 (26.63/18.35)	RQIGTSATVSIQGSP
  409-  423 (27.32/19.05)	RKLQMSATVNIMRTP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      44.45|      14|      15|    1414|    1427|       4
---------------------------------------------------------------------------
 1414- 1427 (25.84/12.04)	SPVVSM.TCITVKSP
 1431- 1445 (18.61/ 6.49)	SPMLALrSPLIVQSP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.56|      28|      31|     152|     182|       5
---------------------------------------------------------------------------
  152-  182 (44.41/41.12)	LKPLQETAK.NLRQALMERrciLD.PSDKLHLQ
  185-  214 (38.15/25.19)	LDTLQQSIKvNSLSSMVER...LDmVSRQLGLQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      97.13|      27|      36|      35|      61|       6
---------------------------------------------------------------------------
   35-   61 (48.49/27.31)	VSNLELLQDMDMMGMQPKASPAGQTPM
   74-  100 (48.64/27.42)	ISNNSSMMDTSSSGVPPQQQQQGGNQM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.50|      16|      24|     425|     447|       9
---------------------------------------------------------------------------
  425-  440 (28.67/28.22)	GKNLPRYGTL.NATNST
  451-  467 (23.83/ 6.34)	GKPIPIFKELlTAIQTT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     100.27|      31|      31|     934|     964|      10
---------------------------------------------------------------------------
  934-  964 (50.99/26.30)	KHVNKLQQQGGKPSVSTMKS..VSTLPKTKKLT
  965-  997 (49.28/25.17)	KSPMDMKKSGDPNKMEKDKSlvIYQPPKTQAAT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      92.97|      29|      31|     701|     731|      11
---------------------------------------------------------------------------
  701-  731 (47.30/39.92)	QCMNYNAPAQTAPIDFISymSNPIFAS.LSQN
  735-  764 (45.66/30.20)	QSQSKNGQGQMTPHSLAA..SSSVLQGlLDQN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.99|      12|      15|     298|     309|      14
---------------------------------------------------------------------------
  298-  309 (20.17/13.54)	QGLISIYNLNAD
  315-  326 (17.82/11.10)	KAFIALQALEAD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     185.81|      62|      66|     474|     539|      15
---------------------------------------------------------------------------
  474-  539 (91.80/82.67)	VTGEsQPiLNLVTQVVSGNQLdCSE..EKG..LFVTLPDQHHCYFMTETAGMEGVLIsSISFTLAS.QLPQ
  542-  608 (94.01/65.25)	VTLR.QQ.LTFNTLVGSCVRL.CSKpvESGsiVFEVSALSHHNLSVSFQHPLDDSLV.TLEFDLRDvSHPQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      58.32|      21|      29|    1040|    1066|      16
---------------------------------------------------------------------------
 1040- 1066 (27.39/31.09)	KSEQMSTKDLLKASssitdiIQK..TASK
 1074- 1096 (30.94/19.25)	SSSGLVPGGGLKLT......INKslTAPK
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP09508 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DIPKPDSLIPAPSNSSSSTNPLLPVGAPSCGLESQQTQ
2) DMDMMGMQPKASPAGQTPMNSNNAPGGMHPSISNNSSMMDTSSSGVPPQQQQQGGNQMGEQNSMPLSQMLASAVN
3) DNPPKFDLKNFQIPKKSKPIEKVETPPPAQSEKNSDLVVESIITTPSTPPQITPPRQPTPQPMVSATPTPTTSSSNPNPLPALTPIGPPPPPIITPSSVPVEPTNNVPRTEEPKIESPVVDIPPPPPPQPLPMAMPQKETIISPESPEPMDTSESIATPQNIPTTPPTTADDAPAAPTEKTSEAETVIPPRSTTPAPPPRSVTPAPPRATTPSPMTATTTTTTNNSVTSAPATVEKVAVEETSTVPPPPPPPASEPSPVVSMTCITVKSPAPNSPMLALRSPLIVQSPHTPFTIDDDLMDEALIGGGPSNNDKAASS
4) LEKKKIKKLDIKHVNKLQQQGGKPSVSTMKSVSTLPKTKKLTKSPMDMKKSGDPNKMEKDKSLVIYQPP
5) MKKKTVLKRPLGQKPKSLTPLKDDLKRPLLKKEEMSKITDTR
6) QNFQSQSQSKNGQGQMTPHSLAASSSVLQGLLDQNNPAKRRKRNKTSTEIQKSPVRQKSPIRQEEPGAAGTAWMT
658
43
1158
923
1103
730
695
117
1474
991
1144
804










Molecular Recognition Features






MoRF SequenceStartStop




1) DDDLMDEALI
2) IINKL
3) IKHVNKLQQ
4) KNFQI
5) LKRPLLKK
6) LNMKKKTVLKRPLG
7) MEKDKSLVIY
1452
1021
933
1166
1127
1101
979
1461
1025
941
1170
1134
1114
988